Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L
<i>Lactiplantibacillus plantarum</i> is best known for its significant adaptive potential and ability to colonize different ecological niches. Different strains of <i>L. plantarum</i> are widely used as probiotics. To characterize the probiotic potential of the novel <i>...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| author | Olga Karaseva Georgii Ozhegov Dilyara Khusnutdinova Maria Siniagina Elizaveta Anisimova Farida Akhatova Rawil Fakhrullin Dina Yarullina |
| author_facet | Olga Karaseva Georgii Ozhegov Dilyara Khusnutdinova Maria Siniagina Elizaveta Anisimova Farida Akhatova Rawil Fakhrullin Dina Yarullina |
| author_sort | Olga Karaseva |
| collection | DOAJ |
| description | <i>Lactiplantibacillus plantarum</i> is best known for its significant adaptive potential and ability to colonize different ecological niches. Different strains of <i>L. plantarum</i> are widely used as probiotics. To characterize the probiotic potential of the novel <i>L. plantarum</i> FCa3L strain isolated from fermented cabbage, we sequenced its whole genome using the Illumina MiSeq platform. This bacterial isolate had a circular chromosome of 3,365,929 bp with 44.3% GC content and a cyclic phage phiX174 of 5386 bp with 44.7% GC content. The results of in vitro studies showed that FCa3L was comparable with the reference probiotic strain <i>L. plantarum</i> 8PA3 in terms of acid and bile tolerance, adhesiveness, H<sub>2</sub>O<sub>2</sub> production, and acidification rate. The strain 8PA3 possessed higher antioxidant activity, while FCa3L demonstrated superior antibacterial properties. The antibiotic resistance of FCa3L was more relevant to the probiotic strain than that of 8PA3, although a number of silent antibiotic resistance genes were identified in its genome. Genomic evidence to support adhesive and antibacterial properties, biosynthesis of bioactive metabolites, and safety of FCa3L was also presented. Thus, this study confirmed the safety and probiotic properties of <i>L. plantarum</i> FCa3L via complete genome and phenotype analysis, suggesting its potential as a probiotic, although further in vivo investigations are still necessary. |
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| last_indexed | 2024-03-11T03:28:02Z |
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| spelling | doaj.art-10fd31c854da4b3d8147dfb8217d87ce2023-11-18T02:33:46ZengMDPI AGMicroorganisms2076-26072023-05-01115123410.3390/microorganisms11051234Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3LOlga Karaseva0Georgii Ozhegov1Dilyara Khusnutdinova2Maria Siniagina3Elizaveta Anisimova4Farida Akhatova5Rawil Fakhrullin6Dina Yarullina7Institute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, RussiaInstitute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, 420008 Kazan, Republic of Tatarstan, Russia<i>Lactiplantibacillus plantarum</i> is best known for its significant adaptive potential and ability to colonize different ecological niches. Different strains of <i>L. plantarum</i> are widely used as probiotics. To characterize the probiotic potential of the novel <i>L. plantarum</i> FCa3L strain isolated from fermented cabbage, we sequenced its whole genome using the Illumina MiSeq platform. This bacterial isolate had a circular chromosome of 3,365,929 bp with 44.3% GC content and a cyclic phage phiX174 of 5386 bp with 44.7% GC content. The results of in vitro studies showed that FCa3L was comparable with the reference probiotic strain <i>L. plantarum</i> 8PA3 in terms of acid and bile tolerance, adhesiveness, H<sub>2</sub>O<sub>2</sub> production, and acidification rate. The strain 8PA3 possessed higher antioxidant activity, while FCa3L demonstrated superior antibacterial properties. The antibiotic resistance of FCa3L was more relevant to the probiotic strain than that of 8PA3, although a number of silent antibiotic resistance genes were identified in its genome. Genomic evidence to support adhesive and antibacterial properties, biosynthesis of bioactive metabolites, and safety of FCa3L was also presented. Thus, this study confirmed the safety and probiotic properties of <i>L. plantarum</i> FCa3L via complete genome and phenotype analysis, suggesting its potential as a probiotic, although further in vivo investigations are still necessary.https://www.mdpi.com/2076-2607/11/5/1234<i>Lactiplantibacillus plantarum</i>draft genome sequencegenome annotationprobiotic properties |
| spellingShingle | Olga Karaseva Georgii Ozhegov Dilyara Khusnutdinova Maria Siniagina Elizaveta Anisimova Farida Akhatova Rawil Fakhrullin Dina Yarullina Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L Microorganisms <i>Lactiplantibacillus plantarum</i> draft genome sequence genome annotation probiotic properties |
| title | Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L |
| title_full | Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L |
| title_fullStr | Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L |
| title_full_unstemmed | Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L |
| title_short | Whole Genome Sequencing of the Novel Probiotic Strain <i>Lactiplantibacillus plantarum</i> FCa3L |
| title_sort | whole genome sequencing of the novel probiotic strain i lactiplantibacillus plantarum i fca3l |
| topic | <i>Lactiplantibacillus plantarum</i> draft genome sequence genome annotation probiotic properties |
| url | https://www.mdpi.com/2076-2607/11/5/1234 |
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