Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration
Calcineurin, a Ca2+/calmodulin-dependent phosphatase, is associated with muscle regeneration via NFATc1/GATA2-dependent pathways. However, it is not clear whether calcineurin preferentially affects the regeneration of slow- or fast-twitch muscles. We investigated the effect of a calcineurin inhibito...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2005-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400009 |
| _version_ | 1818622303928320000 |
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| author | Miyabara E.H. Aoki M.S. Moriscot A.S. |
| author_facet | Miyabara E.H. Aoki M.S. Moriscot A.S. |
| author_sort | Miyabara E.H. |
| collection | DOAJ |
| description | Calcineurin, a Ca2+/calmodulin-dependent phosphatase, is associated with muscle regeneration via NFATc1/GATA2-dependent pathways. However, it is not clear whether calcineurin preferentially affects the regeneration of slow- or fast-twitch muscles. We investigated the effect of a calcineurin inhibitor, cyclosporin A (CsA), on the morphology and fiber diameter of regenerating slow- and fast-twitch muscles. Adult Wistar rats (259.5 ± 9 g) maintained under standard conditions were treated with CsA (20 mg/kg body weight, ip) for 5 days, submitted to cryolesion of soleus and tibialis anterior (TA) muscles on the 6th day, and then treated with CsA for an additional 21 days. The muscles were removed, weighed, frozen, and stored in liquid nitrogen. Cryolesion did not alter the body weight gain of the animals after 21 days of regeneration (P = 0.001) and CsA significantly reduced the body weight gain (15.5%; P = 0.01) during the same period. All treated TA and soleus muscles showed decreased weights (17 and 29%, respectively, P < 0.05). CsA treatment decreased the cross-sectional area of both soleus and TA muscles of cryoinjured animals (TA: 2108 ± 930 vs 792 ± 640 µm²; soleus: 2209 ± 322 vs 764 ± 439 m²; P < 0.001). Histological sections of both muscles stained with Toluidine blue revealed similar regenerative responses after cryolesion. In addition, CsA was able to minimize these responses, i.e., centralized nuclei and split fibers, more efficiently so in TA muscle. These results indicate that calcineurin preferentially plays a role in regeneration of slow-twitch muscle. |
| first_indexed | 2024-12-16T18:23:02Z |
| format | Article |
| id | doaj.art-10fe7130314a4a71825a0895257a6795 |
| institution | Directory Open Access Journal |
| issn | 0100-879X 0034-7310 |
| language | English |
| last_indexed | 2024-12-16T18:23:02Z |
| publishDate | 2005-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-10fe7130314a4a71825a0895257a67952022-12-21T22:21:30ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X0034-73102005-01-01384559563Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regenerationMiyabara E.H.Aoki M.S.Moriscot A.S.Calcineurin, a Ca2+/calmodulin-dependent phosphatase, is associated with muscle regeneration via NFATc1/GATA2-dependent pathways. However, it is not clear whether calcineurin preferentially affects the regeneration of slow- or fast-twitch muscles. We investigated the effect of a calcineurin inhibitor, cyclosporin A (CsA), on the morphology and fiber diameter of regenerating slow- and fast-twitch muscles. Adult Wistar rats (259.5 ± 9 g) maintained under standard conditions were treated with CsA (20 mg/kg body weight, ip) for 5 days, submitted to cryolesion of soleus and tibialis anterior (TA) muscles on the 6th day, and then treated with CsA for an additional 21 days. The muscles were removed, weighed, frozen, and stored in liquid nitrogen. Cryolesion did not alter the body weight gain of the animals after 21 days of regeneration (P = 0.001) and CsA significantly reduced the body weight gain (15.5%; P = 0.01) during the same period. All treated TA and soleus muscles showed decreased weights (17 and 29%, respectively, P < 0.05). CsA treatment decreased the cross-sectional area of both soleus and TA muscles of cryoinjured animals (TA: 2108 ± 930 vs 792 ± 640 µm²; soleus: 2209 ± 322 vs 764 ± 439 m²; P < 0.001). Histological sections of both muscles stained with Toluidine blue revealed similar regenerative responses after cryolesion. In addition, CsA was able to minimize these responses, i.e., centralized nuclei and split fibers, more efficiently so in TA muscle. These results indicate that calcineurin preferentially plays a role in regeneration of slow-twitch muscle.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400009CalcineurinCyclosporin ARegenerationFast and slow twitch muscles |
| spellingShingle | Miyabara E.H. Aoki M.S. Moriscot A.S. Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration Brazilian Journal of Medical and Biological Research Calcineurin Cyclosporin A Regeneration Fast and slow twitch muscles |
| title | Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration |
| title_full | Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration |
| title_fullStr | Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration |
| title_full_unstemmed | Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration |
| title_short | Cyclosporin A preferentially attenuates skeletal slow-twitch muscle regeneration |
| title_sort | cyclosporin a preferentially attenuates skeletal slow twitch muscle regeneration |
| topic | Calcineurin Cyclosporin A Regeneration Fast and slow twitch muscles |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400009 |
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