Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study
Fungal biofilm formation on voice prosthesis (VP) is a major health problem that requires repeated replacement of the prosthesis. <i>Candida albicans</i> is one of the pathogens that frequently inhabits the VP. We proposed that coating VPs with sustained-release varnish (SRV) containing...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/17/5395 |
| _version_ | 1797520938427744256 |
|---|---|
| author | Ronit Vogt Sionov Irith Gati David Kirmayer Michael Friedman Doron Steinberg Menachem Gross |
| author_facet | Ronit Vogt Sionov Irith Gati David Kirmayer Michael Friedman Doron Steinberg Menachem Gross |
| author_sort | Ronit Vogt Sionov |
| collection | DOAJ |
| description | Fungal biofilm formation on voice prosthesis (VP) is a major health problem that requires repeated replacement of the prosthesis. <i>Candida albicans</i> is one of the pathogens that frequently inhabits the VP. We proposed that coating VPs with sustained-release varnish (SRV) containing clotrimazole (CTZ) might prevent fungal biofilm formation. The long-term antifungal activities of SRV-CTZ- versus SRV-placebo-coated VPs was tested daily by measuring the inhibition zone of <i>C. albicans</i> seeded on agar plates or by measuring the fungal viability of <i>C. albicans</i> in suspension. The extent of biofilm formation on coated VPs was analyzed by confocal microscopy and scanning electron microscopy. We observed that SRV-CTZ-coated VPs formed a significant bacterial inhibition zone around the VPs and prevented the growth of <i>C. albicans</i> in suspension during the entire testing period of 60 days. Fungal biofilms were formed on placebo-coated VPs, while no significant biofilms were observed on SRV-CTZ-coated VPs. HPLC analysis shows that CTZ is continuously released during the whole test period of 60 days at a concentration above the minimal fungistatic concentration. In conclusion, coating VPs with an SRV-CTZ film is a potential effective method for prevention of fungal infections and biofilm formation on VPs. |
| first_indexed | 2024-03-10T08:05:34Z |
| format | Article |
| id | doaj.art-10fff57e82e64725839e9e96767abe73 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:05:34Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-10fff57e82e64725839e9e96767abe732023-11-22T11:03:29ZengMDPI AGMolecules1420-30492021-09-012617539510.3390/molecules26175395Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro StudyRonit Vogt Sionov0Irith Gati1David Kirmayer2Michael Friedman3Doron Steinberg4Menachem Gross5The Biofilm Research Laboratory, The Institute of Dental Sciences, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelInstitute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelInstitute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelInstitute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelThe Biofilm Research Laboratory, The Institute of Dental Sciences, The Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, IsraelDepartment of Otolaryngology-Head and Neck Surgery, Hadassah Medical Center, Jerusalem 9112102, IsraelFungal biofilm formation on voice prosthesis (VP) is a major health problem that requires repeated replacement of the prosthesis. <i>Candida albicans</i> is one of the pathogens that frequently inhabits the VP. We proposed that coating VPs with sustained-release varnish (SRV) containing clotrimazole (CTZ) might prevent fungal biofilm formation. The long-term antifungal activities of SRV-CTZ- versus SRV-placebo-coated VPs was tested daily by measuring the inhibition zone of <i>C. albicans</i> seeded on agar plates or by measuring the fungal viability of <i>C. albicans</i> in suspension. The extent of biofilm formation on coated VPs was analyzed by confocal microscopy and scanning electron microscopy. We observed that SRV-CTZ-coated VPs formed a significant bacterial inhibition zone around the VPs and prevented the growth of <i>C. albicans</i> in suspension during the entire testing period of 60 days. Fungal biofilms were formed on placebo-coated VPs, while no significant biofilms were observed on SRV-CTZ-coated VPs. HPLC analysis shows that CTZ is continuously released during the whole test period of 60 days at a concentration above the minimal fungistatic concentration. In conclusion, coating VPs with an SRV-CTZ film is a potential effective method for prevention of fungal infections and biofilm formation on VPs.https://www.mdpi.com/1420-3049/26/17/5395biofilm<i>Candida albicans</i>clotrimazolefungal infectionlaryngeal malignancieslaryngectomy |
| spellingShingle | Ronit Vogt Sionov Irith Gati David Kirmayer Michael Friedman Doron Steinberg Menachem Gross Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study Molecules biofilm <i>Candida albicans</i> clotrimazole fungal infection laryngeal malignancies laryngectomy |
| title | Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study |
| title_full | Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study |
| title_fullStr | Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study |
| title_full_unstemmed | Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study |
| title_short | Voice Prosthesis Coated with Sustained Release Varnish Containing Clotrimazole Shows Long-Term Protection against <i>Candida albicans:</i> An In Vitro Study |
| title_sort | voice prosthesis coated with sustained release varnish containing clotrimazole shows long term protection against i candida albicans i an in vitro study |
| topic | biofilm <i>Candida albicans</i> clotrimazole fungal infection laryngeal malignancies laryngectomy |
| url | https://www.mdpi.com/1420-3049/26/17/5395 |
| work_keys_str_mv | AT ronitvogtsionov voiceprosthesiscoatedwithsustainedreleasevarnishcontainingclotrimazoleshowslongtermprotectionagainsticandidaalbicansianinvitrostudy AT irithgati voiceprosthesiscoatedwithsustainedreleasevarnishcontainingclotrimazoleshowslongtermprotectionagainsticandidaalbicansianinvitrostudy AT davidkirmayer voiceprosthesiscoatedwithsustainedreleasevarnishcontainingclotrimazoleshowslongtermprotectionagainsticandidaalbicansianinvitrostudy AT michaelfriedman voiceprosthesiscoatedwithsustainedreleasevarnishcontainingclotrimazoleshowslongtermprotectionagainsticandidaalbicansianinvitrostudy AT doronsteinberg voiceprosthesiscoatedwithsustainedreleasevarnishcontainingclotrimazoleshowslongtermprotectionagainsticandidaalbicansianinvitrostudy AT menachemgross voiceprosthesiscoatedwithsustainedreleasevarnishcontainingclotrimazoleshowslongtermprotectionagainsticandidaalbicansianinvitrostudy |