The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)
Abstract The apicomplexan parasite Cystoisospora suis is an enteropathogen of suckling piglets with woldwide distribution. As with all coccidian parasites, its lifecycle is characterized by asexual multiplication followed by sexual development with two morphologically distinct cell types that presum...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09714-8 |
| _version_ | 1811272485124964352 |
|---|---|
| author | Teresa Cruz-Bustos Anna Sophia Feix Manolis Lyrakis Marlies Dolezal Bärbel Ruttkowski Anja Joachim |
| author_facet | Teresa Cruz-Bustos Anna Sophia Feix Manolis Lyrakis Marlies Dolezal Bärbel Ruttkowski Anja Joachim |
| author_sort | Teresa Cruz-Bustos |
| collection | DOAJ |
| description | Abstract The apicomplexan parasite Cystoisospora suis is an enteropathogen of suckling piglets with woldwide distribution. As with all coccidian parasites, its lifecycle is characterized by asexual multiplication followed by sexual development with two morphologically distinct cell types that presumably fuse to form a zygote from which the oocyst arises. However, knowledge of the sexual development of C. suis is still limited. To complement previous in vitro studies, we analysed transcriptional profiles at three different time points of development (corresponding to asexual, immature and mature sexual stages) in vitro via RNASeq. Overall, transcription of genes encoding proteins with important roles in gametes biology, oocyst wall biosynthesis, DNA replication and axonema formation as well as proteins with important roles in merozoite biology was identified. A homologue of an oocyst wall tyrosine rich protein of Toxoplasma gondii was expressed in macrogametes and oocysts of C. suis. We evaluated inhibition of sexual development in a host-free culture for C. suis by antiserum specific to this protein to evaluate whether it could be exploited as a candidate for control strategies against C. suis. Based on these data, targets can be defined for future strategies to interrupt parasite transmission during sexual development. |
| first_indexed | 2024-04-12T22:41:08Z |
| format | Article |
| id | doaj.art-11005376de2f445ca7c7e7458a1ca3f7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T22:41:08Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-11005376de2f445ca7c7e7458a1ca3f72022-12-22T03:13:42ZengNature PortfolioScientific Reports2045-23222022-04-0112111710.1038/s41598-022-09714-8The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia)Teresa Cruz-Bustos0Anna Sophia Feix1Manolis Lyrakis2Marlies Dolezal3Bärbel Ruttkowski4Anja Joachim5Institute of Parasitology, Department of Pathobiology, University of Veterinary Medicine ViennaInstitute of Parasitology, Department of Pathobiology, University of Veterinary Medicine ViennaPlatform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine ViennaPlatform for Bioinformatics and Biostatistics, Department of Biomedical Sciences, University of Veterinary Medicine ViennaInstitute of Parasitology, Department of Pathobiology, University of Veterinary Medicine ViennaInstitute of Parasitology, Department of Pathobiology, University of Veterinary Medicine ViennaAbstract The apicomplexan parasite Cystoisospora suis is an enteropathogen of suckling piglets with woldwide distribution. As with all coccidian parasites, its lifecycle is characterized by asexual multiplication followed by sexual development with two morphologically distinct cell types that presumably fuse to form a zygote from which the oocyst arises. However, knowledge of the sexual development of C. suis is still limited. To complement previous in vitro studies, we analysed transcriptional profiles at three different time points of development (corresponding to asexual, immature and mature sexual stages) in vitro via RNASeq. Overall, transcription of genes encoding proteins with important roles in gametes biology, oocyst wall biosynthesis, DNA replication and axonema formation as well as proteins with important roles in merozoite biology was identified. A homologue of an oocyst wall tyrosine rich protein of Toxoplasma gondii was expressed in macrogametes and oocysts of C. suis. We evaluated inhibition of sexual development in a host-free culture for C. suis by antiserum specific to this protein to evaluate whether it could be exploited as a candidate for control strategies against C. suis. Based on these data, targets can be defined for future strategies to interrupt parasite transmission during sexual development.https://doi.org/10.1038/s41598-022-09714-8 |
| spellingShingle | Teresa Cruz-Bustos Anna Sophia Feix Manolis Lyrakis Marlies Dolezal Bärbel Ruttkowski Anja Joachim The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia) Scientific Reports |
| title | The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia) |
| title_full | The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia) |
| title_fullStr | The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia) |
| title_full_unstemmed | The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia) |
| title_short | The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia) |
| title_sort | transcriptome from asexual to sexual in vitro development of cystoisospora suis apicomplexa coccidia |
| url | https://doi.org/10.1038/s41598-022-09714-8 |
| work_keys_str_mv | AT teresacruzbustos thetranscriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT annasophiafeix thetranscriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT manolislyrakis thetranscriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT marliesdolezal thetranscriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT barbelruttkowski thetranscriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT anjajoachim thetranscriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT teresacruzbustos transcriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT annasophiafeix transcriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT manolislyrakis transcriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT marliesdolezal transcriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT barbelruttkowski transcriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia AT anjajoachim transcriptomefromasexualtosexualinvitrodevelopmentofcystoisosporasuisapicomplexacoccidia |