Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography
Abstract Crocodiles are cultured in large numbers in Asia and other places in order to protect wild resources and meet the needs of human life. In this study, crocodile (Crocodylus siamensis) meat proteins were extracted and hydrolyzed into peptides, their antioxidant peptides were isolated and puri...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-08-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-16009-5 |
| _version_ | 1798037957039357952 |
|---|---|
| author | Liu Yang Yan Xing Rui Chen He Ni Hai-Hang Li |
| author_facet | Liu Yang Yan Xing Rui Chen He Ni Hai-Hang Li |
| author_sort | Liu Yang |
| collection | DOAJ |
| description | Abstract Crocodiles are cultured in large numbers in Asia and other places in order to protect wild resources and meet the needs of human life. In this study, crocodile (Crocodylus siamensis) meat proteins were extracted and hydrolyzed into peptides, their antioxidant peptides were isolated and purified by silica gel chromatography and identified by LC/MS. Crocodile meat proteins were optimally extracted with water and hydrolyzed by papain based on the degree of hydrolysis and antioxidant activity. The hydrolysates were fractionated by ultrafiltration into 3 kDa, 3–30 kDa, and ≥ 30 kDa fractions. The 3 kDa fraction showed most antioxidant activity of the hydrolysates. Its active peptides were separated by silica gel column chromatography and purified by silica gel TLC, based on TLC bio-autographic assays of the activity. Four highly active peptides were identified by LC/MS as SSLTIQFVEGQFVDSYDPTIENTFTK, VPPHIY, VAPEEHPVLLTEAPLNPK, and RNGLPGPIGPAG. The identified peptides were synthesized and showed 50% free radical scavenging activities at 1.0 mg/mL, equal or higher to ascorbic acid at 0.5 mg/mL, in both DPPH and ABTS assays. The results indicated that the 3 kDa hydrolyzed peptides of crocodile meat had high antioxidant activity and the active peptides can be effectively separated and purified by silica gel column chromatography and TLC. |
| first_indexed | 2024-04-11T21:33:39Z |
| format | Article |
| id | doaj.art-1100d516e8794b1ba548eedbc9bcad4e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T21:33:39Z |
| publishDate | 2022-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1100d516e8794b1ba548eedbc9bcad4e2022-12-22T04:01:50ZengNature PortfolioScientific Reports2045-23222022-08-0112111010.1038/s41598-022-16009-5Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatographyLiu Yang0Yan Xing1Rui Chen2He Ni3Hai-Hang Li4Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityGuangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityGuangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityGuangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityGuangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal UniversityAbstract Crocodiles are cultured in large numbers in Asia and other places in order to protect wild resources and meet the needs of human life. In this study, crocodile (Crocodylus siamensis) meat proteins were extracted and hydrolyzed into peptides, their antioxidant peptides were isolated and purified by silica gel chromatography and identified by LC/MS. Crocodile meat proteins were optimally extracted with water and hydrolyzed by papain based on the degree of hydrolysis and antioxidant activity. The hydrolysates were fractionated by ultrafiltration into 3 kDa, 3–30 kDa, and ≥ 30 kDa fractions. The 3 kDa fraction showed most antioxidant activity of the hydrolysates. Its active peptides were separated by silica gel column chromatography and purified by silica gel TLC, based on TLC bio-autographic assays of the activity. Four highly active peptides were identified by LC/MS as SSLTIQFVEGQFVDSYDPTIENTFTK, VPPHIY, VAPEEHPVLLTEAPLNPK, and RNGLPGPIGPAG. The identified peptides were synthesized and showed 50% free radical scavenging activities at 1.0 mg/mL, equal or higher to ascorbic acid at 0.5 mg/mL, in both DPPH and ABTS assays. The results indicated that the 3 kDa hydrolyzed peptides of crocodile meat had high antioxidant activity and the active peptides can be effectively separated and purified by silica gel column chromatography and TLC.https://doi.org/10.1038/s41598-022-16009-5 |
| spellingShingle | Liu Yang Yan Xing Rui Chen He Ni Hai-Hang Li Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography Scientific Reports |
| title | Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography |
| title_full | Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography |
| title_fullStr | Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography |
| title_full_unstemmed | Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography |
| title_short | Isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography |
| title_sort | isolation and identification of antioxidative peptides from crocodile meat hydrolysates using silica gel chromatography |
| url | https://doi.org/10.1038/s41598-022-16009-5 |
| work_keys_str_mv | AT liuyang isolationandidentificationofantioxidativepeptidesfromcrocodilemeathydrolysatesusingsilicagelchromatography AT yanxing isolationandidentificationofantioxidativepeptidesfromcrocodilemeathydrolysatesusingsilicagelchromatography AT ruichen isolationandidentificationofantioxidativepeptidesfromcrocodilemeathydrolysatesusingsilicagelchromatography AT heni isolationandidentificationofantioxidativepeptidesfromcrocodilemeathydrolysatesusingsilicagelchromatography AT haihangli isolationandidentificationofantioxidativepeptidesfromcrocodilemeathydrolysatesusingsilicagelchromatography |