Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder
Campylobacter jejuni is an important zoonotic pathogen that is the leading cause of both human foodborne bacterial gastroenteritis worldwide and ovine abortion in the United States. Previous studies have demonstrated that the gallbladder of ruminants is often positive on culture for Campylobacter sp...
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| Format: | Article |
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Frontiers Media S.A.
2019-07-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fvets.2019.00226/full |
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| author | Amanda J. Kreuder Amanda J. Kreuder Victoria Lashley Michael Yaeger Jennifer A. Schleining Paul J. Plummer Paul J. Plummer |
| author_facet | Amanda J. Kreuder Amanda J. Kreuder Victoria Lashley Michael Yaeger Jennifer A. Schleining Paul J. Plummer Paul J. Plummer |
| author_sort | Amanda J. Kreuder |
| collection | DOAJ |
| description | Campylobacter jejuni is an important zoonotic pathogen that is the leading cause of both human foodborne bacterial gastroenteritis worldwide and ovine abortion in the United States. Previous studies have demonstrated that the gallbladder of ruminants is often positive on culture for Campylobacter sp., suggesting that this environment may serve as a chronic nidus of infection for maintenance of disease within populations. The objective of this study was to determine if previously identified putative growth promoting factors of C. jejuni are present within the gallbladder mucosa of sheep and to evaluate for bacterial co-localization of C. jejuni with these compounds following experimental inoculation. Direct gallbladder inoculation with C. jejuni sheep abortion (SA) clone clinical isolate IA3902 followed by immunohistochemical analysis and scanning electron microscopy allowed for identification of C. jejuni at the gallbladder mucosal surface and within the gallbladder submucosal glands. Histochemistry identified several putative Campylobacter growth promoting factors including neutral and acid mucins as well as L-fucose to be present both on the mucosal surface as well as in the gallbladder submucosal glands. In summary, following experimental inoculation of the ovine gallbladder, C. jejuni IA3902 was identified in direct contact with the gallbladder mucosal surface and deep mucosal glands in the same location as several putative growth promoting factors. This suggests the yet to be tested hypothesis that under natural conditions of infection, the gallbladder submucosal glands have the potential to provide a protected niche for chronic carriage of C. jejuni in animal hosts. |
| first_indexed | 2024-12-13T11:34:20Z |
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| id | doaj.art-110361294d1e4c6e845d92260c311ab5 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-13T11:34:20Z |
| publishDate | 2019-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-110361294d1e4c6e845d92260c311ab52022-12-21T23:47:49ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692019-07-01610.3389/fvets.2019.00226461702Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine GallbladderAmanda J. Kreuder0Amanda J. Kreuder1Victoria Lashley2Michael Yaeger3Jennifer A. Schleining4Paul J. Plummer5Paul J. Plummer6Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesDepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United StatesCampylobacter jejuni is an important zoonotic pathogen that is the leading cause of both human foodborne bacterial gastroenteritis worldwide and ovine abortion in the United States. Previous studies have demonstrated that the gallbladder of ruminants is often positive on culture for Campylobacter sp., suggesting that this environment may serve as a chronic nidus of infection for maintenance of disease within populations. The objective of this study was to determine if previously identified putative growth promoting factors of C. jejuni are present within the gallbladder mucosa of sheep and to evaluate for bacterial co-localization of C. jejuni with these compounds following experimental inoculation. Direct gallbladder inoculation with C. jejuni sheep abortion (SA) clone clinical isolate IA3902 followed by immunohistochemical analysis and scanning electron microscopy allowed for identification of C. jejuni at the gallbladder mucosal surface and within the gallbladder submucosal glands. Histochemistry identified several putative Campylobacter growth promoting factors including neutral and acid mucins as well as L-fucose to be present both on the mucosal surface as well as in the gallbladder submucosal glands. In summary, following experimental inoculation of the ovine gallbladder, C. jejuni IA3902 was identified in direct contact with the gallbladder mucosal surface and deep mucosal glands in the same location as several putative growth promoting factors. This suggests the yet to be tested hypothesis that under natural conditions of infection, the gallbladder submucosal glands have the potential to provide a protected niche for chronic carriage of C. jejuni in animal hosts.https://www.frontiersin.org/article/10.3389/fvets.2019.00226/fullCampylobacterovinegallbladderbilegrowth factors |
| spellingShingle | Amanda J. Kreuder Amanda J. Kreuder Victoria Lashley Michael Yaeger Jennifer A. Schleining Paul J. Plummer Paul J. Plummer Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder Frontiers in Veterinary Science Campylobacter ovine gallbladder bile growth factors |
| title | Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder |
| title_full | Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder |
| title_fullStr | Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder |
| title_full_unstemmed | Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder |
| title_short | Histopathology and Spatial Distribution of Putative Growth Factors in Relation to Bacterial Localization of Campylobacter jejuni Within the Ovine Gallbladder |
| title_sort | histopathology and spatial distribution of putative growth factors in relation to bacterial localization of campylobacter jejuni within the ovine gallbladder |
| topic | Campylobacter ovine gallbladder bile growth factors |
| url | https://www.frontiersin.org/article/10.3389/fvets.2019.00226/full |
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