Effects of sprouty4 protein on keratinocyte proliferation and differentiation

Effects of sprouty4 protein on keratinocyte proliferation and differentiation

Objective: To explore the effects of sprouty4 (SPRY4) protein on keratinocyte proliferation and differentiation. Methods: Human immortalized keratinocytes (HaCaT) cells were transfected with SPRY4 protein siRNA, and HaCaT cells without transfection served as controls. Real-time fluorescence quantita...

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Bibliographic Details
Main Authors: Yu-qi HUANG, Li LI, Wei ZHONG, Lei ZHU, Jing-xia LIN, Jiao ZHANG, Yong-feng CHEN
Format: Article
Language:zho
Published: editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology 2020-06-01
Series:Pifu-xingbing zhenliaoxue zazhi
Subjects:
psoriasis vulgaris
sprouty4 protein
keratinocytes
proliferation
differentiation
Online Access:http://pfxbzlx.gdvdc.com/CN/abstract/abstract1820.shtml
Description
Summary:Objective: To explore the effects of sprouty4 (SPRY4) protein on keratinocyte proliferation and differentiation. Methods: Human immortalized keratinocytes (HaCaT) cells were transfected with SPRY4 protein siRNA, and HaCaT cells without transfection served as controls. Real-time fluorescence quantitative PCR (RT-qPCR) was used to assess expression levels of mRNA for HaCaT cells differentiation (Involucrin, CK1 and CK10), and the CCK-8 assay was used to measure HaCaT cell proliferation. Results: RT-qPCR results showed 94.75% reduction in expression levels of SPRY4 mRNA in comparison to the controls. Moreover, knockdown of SPRY4 decreased expression levels of involucrin, CK1 and CK10 mRNA, while stimulating HaCaT cells proliferation. Conclusion: SPRY4 protein stimulates keratinocyte proliferation, while inhibiting differentiation.
ISSN:1674-8468

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