MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria
This study used MALDI-TOF MS and molecular tools to identify tick species infesting camels from Tamanrasset in southern Algeria and to investigate their associated microorganisms. Ninety-one adult ticks were collected from nine camels and were morphologically identified as <i>Hyalomma</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/11/2178 |
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| author | Fatima Zohra Hamlili Laroche Maureen Adama Zan Diarra Ismail Lafri Brahim Gassen Boubaker Boutefna Bernard Davoust Idir Bitam Philippe Parola |
| author_facet | Fatima Zohra Hamlili Laroche Maureen Adama Zan Diarra Ismail Lafri Brahim Gassen Boubaker Boutefna Bernard Davoust Idir Bitam Philippe Parola |
| author_sort | Fatima Zohra Hamlili |
| collection | DOAJ |
| description | This study used MALDI-TOF MS and molecular tools to identify tick species infesting camels from Tamanrasset in southern Algeria and to investigate their associated microorganisms. Ninety-one adult ticks were collected from nine camels and were morphologically identified as <i>Hyalomma</i> spp., <i>Hyalomma dromedarii</i>, <i>Hyalomma excavatum</i>, <i>Hyalomma impeltatum</i> and <i>Hyalomma anatolicum</i>. Next, the legs of all ticks were subjected to MALDI-TOF MS, and 88/91 specimens provided good-quality MS spectra. Our homemade MALDI-TOF MS arthropod spectra database was then updated with the new MS spectra of 14 specimens of molecularly confirmed species in this study. The spectra of the remaining tick specimens not included in the MS database were queried against the upgraded database. All 74 specimens were correctly identified by MALDI-TOF MS, with logarithmic score values ranging from 1.701 to 2.507, with median and mean values of 2.199 and 2.172 ± 0.169, respectively. One <i>H. impeltatum</i> and one <i>H. dromedarii</i> (2/91; 2.20%) tested positive by qPCR for <i>Coxiella burnetii</i>, the agent of Q fever. We also report the first detection of an <i>Anaplasma</i> sp. close to <i>A. platys</i> in <i>H. dromedarii</i> in Algeria and a potentially new <i>Ehrlichia</i> sp. in <i>H. impeltatum</i>. |
| first_indexed | 2024-03-09T18:49:35Z |
| format | Article |
| id | doaj.art-1108d15a52984a058f98649c50522c90 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T18:49:35Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1108d15a52984a058f98649c50522c902023-11-24T05:57:01ZengMDPI AGMicroorganisms2076-26072022-11-011011217810.3390/microorganisms10112178MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern AlgeriaFatima Zohra Hamlili0Laroche Maureen1Adama Zan Diarra2Ismail Lafri3Brahim Gassen4Boubaker Boutefna5Bernard Davoust6Idir Bitam7Philippe Parola8IHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, FranceIHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, FranceIHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, FranceLaboratoire des Biotechnologies Liées à la Reproduction Animale, Institut des Sciences Vétérinaires, Université de Blida 1, Blida 09000, AlgeriaEtablissement Public Hospitalier de Tamanrasset, Tamanrasset 11008, AlgeriaInspection Vétérinaires, Direction des Services Agricoles, Tamanrasset 11008, AlgeriaIHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, FranceLaboratoire Mixte International REMEDIER, VITROME, IRD, 13005 Marseille, FranceIHU Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005 Marseille, FranceThis study used MALDI-TOF MS and molecular tools to identify tick species infesting camels from Tamanrasset in southern Algeria and to investigate their associated microorganisms. Ninety-one adult ticks were collected from nine camels and were morphologically identified as <i>Hyalomma</i> spp., <i>Hyalomma dromedarii</i>, <i>Hyalomma excavatum</i>, <i>Hyalomma impeltatum</i> and <i>Hyalomma anatolicum</i>. Next, the legs of all ticks were subjected to MALDI-TOF MS, and 88/91 specimens provided good-quality MS spectra. Our homemade MALDI-TOF MS arthropod spectra database was then updated with the new MS spectra of 14 specimens of molecularly confirmed species in this study. The spectra of the remaining tick specimens not included in the MS database were queried against the upgraded database. All 74 specimens were correctly identified by MALDI-TOF MS, with logarithmic score values ranging from 1.701 to 2.507, with median and mean values of 2.199 and 2.172 ± 0.169, respectively. One <i>H. impeltatum</i> and one <i>H. dromedarii</i> (2/91; 2.20%) tested positive by qPCR for <i>Coxiella burnetii</i>, the agent of Q fever. We also report the first detection of an <i>Anaplasma</i> sp. close to <i>A. platys</i> in <i>H. dromedarii</i> in Algeria and a potentially new <i>Ehrlichia</i> sp. in <i>H. impeltatum</i>.https://www.mdpi.com/2076-2607/10/11/2178MALDI-TOF MSticks<i>Hyalomma</i>camels<i>Anaplasma platys</i><i>Coxiella burnetii</i> |
| spellingShingle | Fatima Zohra Hamlili Laroche Maureen Adama Zan Diarra Ismail Lafri Brahim Gassen Boubaker Boutefna Bernard Davoust Idir Bitam Philippe Parola MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria Microorganisms MALDI-TOF MS ticks <i>Hyalomma</i> camels <i>Anaplasma platys</i> <i>Coxiella burnetii</i> |
| title | MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria |
| title_full | MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria |
| title_fullStr | MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria |
| title_full_unstemmed | MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria |
| title_short | MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria |
| title_sort | maldi tof ms identification of dromedary camel ticks and detection of associated microorganisms southern algeria |
| topic | MALDI-TOF MS ticks <i>Hyalomma</i> camels <i>Anaplasma platys</i> <i>Coxiella burnetii</i> |
| url | https://www.mdpi.com/2076-2607/10/11/2178 |
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