High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani
To identify Nicotiana tabacum genes involved in resistance and susceptibility to the phytopathogenic fungus Rhizoctonia solani, transcripts were generated (by subtractive libraries), which were differentially expressed in each interaction. This enabled isolation of a gene coding for a protein kinase...
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| Format: | Article |
| Language: | English |
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Elfos Scientiae
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| Series: | Biotecnología Aplicada |
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| Online Access: | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000400010&lng=en&tlng=en |
| _version_ | 1818515754046193664 |
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| author | Orlando Borrás Osmani Chacón Marleny González Roxana Portieles Ernesto González Merardo Pujol |
| author_facet | Orlando Borrás Osmani Chacón Marleny González Roxana Portieles Ernesto González Merardo Pujol |
| author_sort | Orlando Borrás |
| collection | DOAJ |
| description | To identify Nicotiana tabacum genes involved in resistance and susceptibility to the phytopathogenic fungus Rhizoctonia solani, transcripts were generated (by subtractive libraries), which were differentially expressed in each interaction. This enabled isolation of a gene coding for a protein kinase that becomes silent during the susceptibility interaction and is activated during resistance. The expression of this gene in tobacco plants significantly increased resistance against an aggressive isolate of R. solani. However, silencing the gene drastically reduced resistance to a non-aggressive isolate of R. solani. Besides, genes such as: superoxide dismutase, hsr203j, chitinases and phenylalanine ammonia lyase, related with resistance to tobacco plant diseases, in which the gene coding for the protein kinase is overexpressed or silenced, were evaluated. This gene can be used to design a strategy of resistance to R. solani in tobacco cultures or other plants of the Solanaceae family, susceptible to this phytopathogen. |
| first_indexed | 2024-12-11T00:33:00Z |
| format | Article |
| id | doaj.art-1108d42b3a6e469583b01df12b4384aa |
| institution | Directory Open Access Journal |
| issn | 1027-2852 |
| language | English |
| last_indexed | 2024-12-11T00:33:00Z |
| publisher | Elfos Scientiae |
| record_format | Article |
| series | Biotecnología Aplicada |
| spelling | doaj.art-1108d42b3a6e469583b01df12b4384aa2022-12-22T01:27:16ZengElfos ScientiaeBiotecnología Aplicada1027-2852294279281S1027-28522012000400010High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solaniOrlando Borrás0Osmani Chacón1Marleny González2Roxana Portieles3Ernesto González4Merardo Pujol5Centro de Ingeniería Genética y BiotecnologíaInstituto de Investigaciones del Tabaco, IITInstituto Nacional de Investigaciones en Sanidad Vegetal, InisavCentro de Ingeniería Genética y BiotecnologíaCentro de Ingeniería Genética y BiotecnologíaCentro de Ingeniería Genética y BiotecnologíaTo identify Nicotiana tabacum genes involved in resistance and susceptibility to the phytopathogenic fungus Rhizoctonia solani, transcripts were generated (by subtractive libraries), which were differentially expressed in each interaction. This enabled isolation of a gene coding for a protein kinase that becomes silent during the susceptibility interaction and is activated during resistance. The expression of this gene in tobacco plants significantly increased resistance against an aggressive isolate of R. solani. However, silencing the gene drastically reduced resistance to a non-aggressive isolate of R. solani. Besides, genes such as: superoxide dismutase, hsr203j, chitinases and phenylalanine ammonia lyase, related with resistance to tobacco plant diseases, in which the gene coding for the protein kinase is overexpressed or silenced, were evaluated. This gene can be used to design a strategy of resistance to R. solani in tobacco cultures or other plants of the Solanaceae family, susceptible to this phytopathogen.http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000400010&lng=en&tlng=enntpkrhizoctonia solanihongonicotiana tabacumresistenciafitopatógenosilenciamiento de genes |
| spellingShingle | Orlando Borrás Osmani Chacón Marleny González Roxana Portieles Ernesto González Merardo Pujol High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani Biotecnología Aplicada ntpk rhizoctonia solani hongo nicotiana tabacum resistencia fitopatógeno silenciamiento de genes |
| title | High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani |
| title_full | High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani |
| title_fullStr | High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani |
| title_full_unstemmed | High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani |
| title_short | High protection of protein kinase NtPK against the phytopatogenic fungus Rhizoctonia solani |
| title_sort | high protection of protein kinase ntpk against the phytopatogenic fungus rhizoctonia solani |
| topic | ntpk rhizoctonia solani hongo nicotiana tabacum resistencia fitopatógeno silenciamiento de genes |
| url | http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522012000400010&lng=en&tlng=en |
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