Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize
Reactive oxygen species (ROS) are an important part of adaptation to biotic and abiotic stresses and regulate seed germination through positive or negative signaling. Seed adaptation to abiotic stress may be mediated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The effe...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15557 |
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| author | Wei-Qing Li Jia-Yu Li Yi-Fei Zhang Wen-Qi Luo Yi Dou Song Yu |
| author_facet | Wei-Qing Li Jia-Yu Li Yi-Fei Zhang Wen-Qi Luo Yi Dou Song Yu |
| author_sort | Wei-Qing Li |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) are an important part of adaptation to biotic and abiotic stresses and regulate seed germination through positive or negative signaling. Seed adaptation to abiotic stress may be mediated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The effects of the ROS scavenger N,N′-dimethylthiourea (DMTU) on maize seed germination through endogenous H<sub>2</sub>O<sub>2</sub> regulation is unclear. In this study, we investigated the effects of different doses of DMTU on seed endogenous H<sub>2</sub>O<sub>2</sub> and radicle development parameters using two maize varieties (ZD958 and DMY1). The inhibitory effect of DMTU on the germination rate and radicle growth was dose-dependent. The inhibitory effect of DMTU on radicle growth ceased after transferring maize seeds from DMTU to a water medium. Histochemical analyses showed that DMTU eliminated stable H<sub>2</sub>O<sub>2</sub> accumulation in the radicle sheaths and radicles. The activity of antioxidant enzyme and the expression of antioxidant enzyme-related genes (<i>ZmAPX2</i> and <i>ZmCAT2</i>) were reduced in maize seeds cultured with DMTU compared with normal culture conditions (0 mmol·dm<sup>−3</sup> DMTU). We suggest the use of 200 mmol·dm<sup>−3</sup> DMTU as an H<sub>2</sub>O<sub>2</sub> scavenger to study the ROS equilibrium mechanisms during the germination of maize seeds, assisting in the future with the efficient development of plant growth regulators to enhance the seed germination performance of test maize varieties under abiotic stress. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:29:22Z |
| publishDate | 2023-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-110e1dcdb26b4abcab40e27dbb49d5c22023-11-10T15:04:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211555710.3390/ijms242115557Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of MaizeWei-Qing Li0Jia-Yu Li1Yi-Fei Zhang2Wen-Qi Luo3Yi Dou4Song Yu5College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, ChinaReactive oxygen species (ROS) are an important part of adaptation to biotic and abiotic stresses and regulate seed germination through positive or negative signaling. Seed adaptation to abiotic stress may be mediated by hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). The effects of the ROS scavenger N,N′-dimethylthiourea (DMTU) on maize seed germination through endogenous H<sub>2</sub>O<sub>2</sub> regulation is unclear. In this study, we investigated the effects of different doses of DMTU on seed endogenous H<sub>2</sub>O<sub>2</sub> and radicle development parameters using two maize varieties (ZD958 and DMY1). The inhibitory effect of DMTU on the germination rate and radicle growth was dose-dependent. The inhibitory effect of DMTU on radicle growth ceased after transferring maize seeds from DMTU to a water medium. Histochemical analyses showed that DMTU eliminated stable H<sub>2</sub>O<sub>2</sub> accumulation in the radicle sheaths and radicles. The activity of antioxidant enzyme and the expression of antioxidant enzyme-related genes (<i>ZmAPX2</i> and <i>ZmCAT2</i>) were reduced in maize seeds cultured with DMTU compared with normal culture conditions (0 mmol·dm<sup>−3</sup> DMTU). We suggest the use of 200 mmol·dm<sup>−3</sup> DMTU as an H<sub>2</sub>O<sub>2</sub> scavenger to study the ROS equilibrium mechanisms during the germination of maize seeds, assisting in the future with the efficient development of plant growth regulators to enhance the seed germination performance of test maize varieties under abiotic stress.https://www.mdpi.com/1422-0067/24/21/15557seed germinationDMTUH<sub>2</sub>O<sub>2</sub>maizegene expressionradicle elongation |
| spellingShingle | Wei-Qing Li Jia-Yu Li Yi-Fei Zhang Wen-Qi Luo Yi Dou Song Yu Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize International Journal of Molecular Sciences seed germination DMTU H<sub>2</sub>O<sub>2</sub> maize gene expression radicle elongation |
| title | Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize |
| title_full | Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize |
| title_fullStr | Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize |
| title_full_unstemmed | Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize |
| title_short | Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize |
| title_sort | effect of reactive oxygen scavenger n n dimethylthiourea dmtu on seed germination and radicle elongation of maize |
| topic | seed germination DMTU H<sub>2</sub>O<sub>2</sub> maize gene expression radicle elongation |
| url | https://www.mdpi.com/1422-0067/24/21/15557 |
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