Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)
Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2002-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200210-0020_improved-diagnostic-tools-for-the-certification-of-strawberry-propagation-material-the-use-of-pcr-and-nasba-f.php |
| Summary: | Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBV-NASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR. |
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| ISSN: | 1212-2580 1805-9341 |