Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)
Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200210-0020_improved-diagnostic-tools-for-the-certification-of-strawberry-propagation-material-the-use-of-pcr-and-nasba-f.php |
| _version_ | 1797898609144889344 |
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| author | D. Vašková J. Špak |
| author_facet | D. Vašková J. Špak |
| author_sort | D. Vašková |
| collection | DOAJ |
| description | Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBV-NASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR. |
| first_indexed | 2024-04-10T08:16:58Z |
| format | Article |
| id | doaj.art-110f5c6a6c194d8abab9039950c747ef |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:16:58Z |
| publishDate | 2002-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-110f5c6a6c194d8abab9039950c747ef2023-02-23T03:43:39ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412002-06-0138SI 1 - 6th Conf EFPPS24S2710.17221/10313-PPSpps-200210-0020Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)D. Vašková0J. Špak1*Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czech Republic*Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, 370 05 České Budějovice, Czech RepublicControl of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBV-NASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR.https://pps.agriculturejournals.cz/artkey/pps-200210-0020_improved-diagnostic-tools-for-the-certification-of-strawberry-propagation-material-the-use-of-pcr-and-nasba-f.phpstrawberry vein banding virussvbvcaulimovirusfragaria spp.nucleic acid sequence based amplificationnasbamolecular beacon |
| spellingShingle | D. Vašková J. Špak Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV) Plant Protection Science strawberry vein banding virus svbv caulimovirus fragaria spp. nucleic acid sequence based amplification nasba molecular beacon |
| title | Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV) |
| title_full | Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV) |
| title_fullStr | Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV) |
| title_full_unstemmed | Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV) |
| title_short | Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV) |
| title_sort | improved diagnostic tools for the certification of strawberry propagation material the use of pcr and nasba for detection of strawberry vein banding virus svbv |
| topic | strawberry vein banding virus svbv caulimovirus fragaria spp. nucleic acid sequence based amplification nasba molecular beacon |
| url | https://pps.agriculturejournals.cz/artkey/pps-200210-0020_improved-diagnostic-tools-for-the-certification-of-strawberry-propagation-material-the-use-of-pcr-and-nasba-f.php |
| work_keys_str_mv | AT dvaskova improveddiagnostictoolsforthecertificationofstrawberrypropagationmaterialtheuseofpcrandnasbafordetectionofstrawberryveinbandingvirussvbv AT jspak improveddiagnostictoolsforthecertificationofstrawberrypropagationmaterialtheuseofpcrandnasbafordetectionofstrawberryveinbandingvirussvbv |