Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling
Hypertension is one of the most common cardiovascular diseases, resulting in serious complications such as cardiovascular damage and chronic kidney disease. Tianshu capsule (TSC), composed of Chuanxiong (Ligusticum chuanxiong Hort) and Tianma (Gastrodiaelata Blume), has been widely used to treat the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00989/full |
| _version_ | 1818140955107131392 |
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| author | Jian Gao Jian Gao Tieshan Wang Chao Wang Shuai Wang Wei Wang Di Ma Yongbiao Li Haibin Zhao Haibin Zhao Jianxin Chen |
| author_facet | Jian Gao Jian Gao Tieshan Wang Chao Wang Shuai Wang Wei Wang Di Ma Yongbiao Li Haibin Zhao Haibin Zhao Jianxin Chen |
| author_sort | Jian Gao |
| collection | DOAJ |
| description | Hypertension is one of the most common cardiovascular diseases, resulting in serious complications such as cardiovascular damage and chronic kidney disease. Tianshu capsule (TSC), composed of Chuanxiong (Ligusticum chuanxiong Hort) and Tianma (Gastrodiaelata Blume), has been widely used to treat the blood stasis type of headache and migraine in clinic. Results of previous research showed its antihypertensive effects, but the underlying mechanisms were still unclear. The purpose of this study was to evaluate the antihypertensive effect of TSC on spontaneously hypertensive rats by 1H NMR-based metabonomics and enzyme-linked immunosorbent assay (ELIAS), explore potential biomarkers and targets, and probe the potential mechanism of TSC on antihypertensive treatment. The results showed that TSC could decrease the product of oxidative stress (MDA) and enhance the activities of SOD and GSH-Px, down-regulate the expression of enzymes (LDHA, PKM2 and HK2) related to glycolysis, and perturb the levels of a series of amino acids (isoleucine, alanine, asparagine, citrate, etc.) and pathways. Multivariate statistical analyses showed remarkable changes in some endogenous metabolites after administrating TSC related to oxidative stress, amino acid metabolism and energy metabolism disturbances. Some enzymes (alanine-glyoxylate aminotransferase-2, tyrosine hydroxylase, dopa decarboxylase, etc.) related to metabolic biomarkers were predicted as the potential targets of TSC treatment on SHRs. The discoveries are helpful to understand the antihypertensive mechanism of TSC and provide theoretical evidence for its future research, development and clinical use. |
| first_indexed | 2024-12-11T10:52:12Z |
| format | Article |
| id | doaj.art-110fea97a8ff48249ef0b74b2838b57a |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-11T10:52:12Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-110fea97a8ff48249ef0b74b2838b57a2022-12-22T01:10:14ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-09-011010.3389/fphar.2019.00989446182Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic ProfilingJian Gao0Jian Gao1Tieshan Wang2Chao Wang3Shuai Wang4Wei Wang5Di Ma6Yongbiao Li7Haibin Zhao8Haibin Zhao9Jianxin Chen10The Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaBeijing University of Chinese Medicine, Beijing, ChinaBeijing University of Chinese Medicine, Beijing, ChinaDongfang Hospital, Beijing University of Chinese Medicine, Beijing, ChinaThe Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaThe Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaThe Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu, ChinaThe Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, ChinaSchool of Life Science and Engineering, Southwest Jiaotong University, Chengdu, ChinaBeijing University of Chinese Medicine, Beijing, ChinaHypertension is one of the most common cardiovascular diseases, resulting in serious complications such as cardiovascular damage and chronic kidney disease. Tianshu capsule (TSC), composed of Chuanxiong (Ligusticum chuanxiong Hort) and Tianma (Gastrodiaelata Blume), has been widely used to treat the blood stasis type of headache and migraine in clinic. Results of previous research showed its antihypertensive effects, but the underlying mechanisms were still unclear. The purpose of this study was to evaluate the antihypertensive effect of TSC on spontaneously hypertensive rats by 1H NMR-based metabonomics and enzyme-linked immunosorbent assay (ELIAS), explore potential biomarkers and targets, and probe the potential mechanism of TSC on antihypertensive treatment. The results showed that TSC could decrease the product of oxidative stress (MDA) and enhance the activities of SOD and GSH-Px, down-regulate the expression of enzymes (LDHA, PKM2 and HK2) related to glycolysis, and perturb the levels of a series of amino acids (isoleucine, alanine, asparagine, citrate, etc.) and pathways. Multivariate statistical analyses showed remarkable changes in some endogenous metabolites after administrating TSC related to oxidative stress, amino acid metabolism and energy metabolism disturbances. Some enzymes (alanine-glyoxylate aminotransferase-2, tyrosine hydroxylase, dopa decarboxylase, etc.) related to metabolic biomarkers were predicted as the potential targets of TSC treatment on SHRs. The discoveries are helpful to understand the antihypertensive mechanism of TSC and provide theoretical evidence for its future research, development and clinical use.https://www.frontiersin.org/article/10.3389/fphar.2019.00989/fullTianshu capsulehypertensive1H-NMRmetabolismSHR |
| spellingShingle | Jian Gao Jian Gao Tieshan Wang Chao Wang Shuai Wang Wei Wang Di Ma Yongbiao Li Haibin Zhao Haibin Zhao Jianxin Chen Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling Frontiers in Pharmacology Tianshu capsule hypertensive 1H-NMR metabolism SHR |
| title | Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling |
| title_full | Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling |
| title_fullStr | Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling |
| title_full_unstemmed | Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling |
| title_short | Effects of Tianshu Capsule on Spontaneously Hypertensive Rats as Revealed by 1H-NMR-Based Metabolic Profiling |
| title_sort | effects of tianshu capsule on spontaneously hypertensive rats as revealed by 1h nmr based metabolic profiling |
| topic | Tianshu capsule hypertensive 1H-NMR metabolism SHR |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.00989/full |
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