Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells
Cryopreservation of placenta tissue for long-term storage provides the opportunity in the future to isolate mesenchymal stromal cells that could be used for cell therapy and regenerative medicine. Despite being widely used, the established cryopreservation protocols for freezing and thawing still ra...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1140781/full |
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| author | Rūta Navakauskienė Deimantė Žukauskaitė Veronika Viktorija Borutinskaitė Tetiana Bukreieva Tetiana Bukreieva Giedrė Skliutė Giedrė Skliutė Elvina Valatkaitė Aistė Zentelytė Lina Piešinienė Volodymyr Shablii Volodymyr Shablii |
| author_facet | Rūta Navakauskienė Deimantė Žukauskaitė Veronika Viktorija Borutinskaitė Tetiana Bukreieva Tetiana Bukreieva Giedrė Skliutė Giedrė Skliutė Elvina Valatkaitė Aistė Zentelytė Lina Piešinienė Volodymyr Shablii Volodymyr Shablii |
| author_sort | Rūta Navakauskienė |
| collection | DOAJ |
| description | Cryopreservation of placenta tissue for long-term storage provides the opportunity in the future to isolate mesenchymal stromal cells that could be used for cell therapy and regenerative medicine. Despite being widely used, the established cryopreservation protocols for freezing and thawing still raise concerns about their impact on molecular characteristics, such as epigenetic regulation. In our study, we compared the characteristics of human placental mesenchymal stromal cells (hPMSCs) isolated from fresh (native) and cryopreserved (cryo) placenta tissue. We assessed and compared the characteristics of native and cryo hPMSCs such as morphology, metabolic and differentiation potential, expression of cell surface markers, and transcriptome. No significant changes in immunophenotype and differentiation capacity between native and cryo cells were observed. Furthermore, we investigated the epigenetic changes and demonstrated that both native and cryo hPMSCs express only slight variations in the epigenetic profile, including miRNA levels, DNA methylation, and histone modifications. Nevertheless, transcriptome analysis defined the upregulation of early-senescence state-associated genes in hPMSCs after cryopreservation. We also evaluated the ability of hPMSCs to improve pregnancy outcomes in mouse models. Improved pregnancy outcomes in a mouse model confirmed that isolated placental cells both from native and cryo tissue have a positive effect on the restoration of the reproductive system. Still, the native hPMSCs possess better capacity (up to 66%) in comparison with cryo hPMSCs (up to 33%) to restore fertility in mice with premature ovarian failure. Our study demonstrates that placental tissue can be cryopreserved for long-term storage with the possibility to isolate mesenchymal stromal cells that retain characteristics suitable for therapeutic use. |
| first_indexed | 2024-04-09T18:15:16Z |
| format | Article |
| id | doaj.art-111110c10cc34dfeb751591847324c1e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-09T18:15:16Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-111110c10cc34dfeb751591847324c1e2023-04-13T04:41:27ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-04-011110.3389/fbioe.2023.11407811140781Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cellsRūta Navakauskienė0Deimantė Žukauskaitė1Veronika Viktorija Borutinskaitė2Tetiana Bukreieva3Tetiana Bukreieva4Giedrė Skliutė5Giedrė Skliutė6Elvina Valatkaitė7Aistė Zentelytė8Lina Piešinienė9Volodymyr Shablii10Volodymyr Shablii11Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaDepartment of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaDepartment of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaLaboratory of Biosynthesis of Nucleic Acids, Department of Functional Genomics, Institute of Molecular Biology and Genetics, National Academy of Science, Kyiv, UkrainePlacenta Stem Cell Laboratory, Cryobank, Institute of Cell Therapy, Kyiv, UkraineDepartment of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaNanodiagnostika, Ltd., Vilnius, LithuaniaDepartment of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaDepartment of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Vilnius, LithuaniaNanodiagnostika, Ltd., Vilnius, LithuaniaLaboratory of Biosynthesis of Nucleic Acids, Department of Functional Genomics, Institute of Molecular Biology and Genetics, National Academy of Science, Kyiv, UkrainePlacenta Stem Cell Laboratory, Cryobank, Institute of Cell Therapy, Kyiv, UkraineCryopreservation of placenta tissue for long-term storage provides the opportunity in the future to isolate mesenchymal stromal cells that could be used for cell therapy and regenerative medicine. Despite being widely used, the established cryopreservation protocols for freezing and thawing still raise concerns about their impact on molecular characteristics, such as epigenetic regulation. In our study, we compared the characteristics of human placental mesenchymal stromal cells (hPMSCs) isolated from fresh (native) and cryopreserved (cryo) placenta tissue. We assessed and compared the characteristics of native and cryo hPMSCs such as morphology, metabolic and differentiation potential, expression of cell surface markers, and transcriptome. No significant changes in immunophenotype and differentiation capacity between native and cryo cells were observed. Furthermore, we investigated the epigenetic changes and demonstrated that both native and cryo hPMSCs express only slight variations in the epigenetic profile, including miRNA levels, DNA methylation, and histone modifications. Nevertheless, transcriptome analysis defined the upregulation of early-senescence state-associated genes in hPMSCs after cryopreservation. We also evaluated the ability of hPMSCs to improve pregnancy outcomes in mouse models. Improved pregnancy outcomes in a mouse model confirmed that isolated placental cells both from native and cryo tissue have a positive effect on the restoration of the reproductive system. Still, the native hPMSCs possess better capacity (up to 66%) in comparison with cryo hPMSCs (up to 33%) to restore fertility in mice with premature ovarian failure. Our study demonstrates that placental tissue can be cryopreserved for long-term storage with the possibility to isolate mesenchymal stromal cells that retain characteristics suitable for therapeutic use.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1140781/fullplacentastromal cellsplacenta-derivativestissue cryopreservationanimal study |
| spellingShingle | Rūta Navakauskienė Deimantė Žukauskaitė Veronika Viktorija Borutinskaitė Tetiana Bukreieva Tetiana Bukreieva Giedrė Skliutė Giedrė Skliutė Elvina Valatkaitė Aistė Zentelytė Lina Piešinienė Volodymyr Shablii Volodymyr Shablii Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells Frontiers in Bioengineering and Biotechnology placenta stromal cells placenta-derivatives tissue cryopreservation animal study |
| title | Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells |
| title_full | Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells |
| title_fullStr | Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells |
| title_full_unstemmed | Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells |
| title_short | Effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells |
| title_sort | effects of human placenta cryopreservation on molecular characteristics of placental mesenchymal stromal cells |
| topic | placenta stromal cells placenta-derivatives tissue cryopreservation animal study |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1140781/full |
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