In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants
Abstract Objective Plants in the Annonaceae family are known for having abundant biologically active secondary metabolites. They have been used in alternative drugs for various diseases in several countries, for instance, the bark of Cananga odorata (Lam.) Hook and Thomson is used for Ophthalmic inf...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-023-06664-w |
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| author | Pathrapol Lithanatudom Kriangkrai Chawansuntati Chalermpong Saenjum Tanawat Chaowasku Kritsadee Rattanathammethee Boonsong Wungsintaweekul Maslin Osathanunkul Jiraprapa Wipasa |
| author_facet | Pathrapol Lithanatudom Kriangkrai Chawansuntati Chalermpong Saenjum Tanawat Chaowasku Kritsadee Rattanathammethee Boonsong Wungsintaweekul Maslin Osathanunkul Jiraprapa Wipasa |
| author_sort | Pathrapol Lithanatudom |
| collection | DOAJ |
| description | Abstract Objective Plants in the Annonaceae family are known for having abundant biologically active secondary metabolites. They have been used in alternative drugs for various diseases in several countries, for instance, the bark of Cananga odorata (Lam.) Hook and Thomson is used for Ophthalmic inflammation and wound healing in Malaysia. Extracts from the leaves and stems of four Annonaceae plants, namely Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Dasymaschalon sp., Artabotrys burmanicus A.DC, and Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders were investigated for growth inhibitory activity against blood-stage Plasmodium falciparum growth in vitro and for non-specific cytotoxicity against normal peripheral blood mononuclear cells (PBMCs). Antimalarial activity was assessed by invasion inhibition assay and the percentage of infected red blood cells on blood smears were determined. Cytotoxicity was tested by culturing PBMCs with the extracts, and viabilities were determined by Annexin V/propidium iodide staining. Results A. burmanicus stem extract and M. modestum leaf extract were capable of inhibiting growth of P. falciparum when used at 200 µg/mL compared to chloroquine. The extracts at effective concentrations, did not affect the viability of PBMCs. These results support further need for characterization of active compounds from specific Annonaceae plants in order to exploit their components for potential malaria treatment. |
| first_indexed | 2024-03-08T19:49:01Z |
| format | Article |
| id | doaj.art-111204642b4748bab8ea1c4375783b6b |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-08T19:49:01Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-111204642b4748bab8ea1c4375783b6b2023-12-24T12:09:04ZengBMCBMC Research Notes1756-05002023-12-011611710.1186/s13104-023-06664-wIn-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plantsPathrapol Lithanatudom0Kriangkrai Chawansuntati1Chalermpong Saenjum2Tanawat Chaowasku3Kritsadee Rattanathammethee4Boonsong Wungsintaweekul5Maslin Osathanunkul6Jiraprapa Wipasa7Department of Biology, Faculty of Science, Chiang Mai UniversityResearch Institute for Health Sciences, Chiang Mai UniversityDepartment of Pharmaceutical Science, Faculty of Pharmacy, Chiang Mai UniversityDepartment of Biology, Faculty of Science, Chiang Mai UniversityResearch Institute for Health Sciences, Chiang Mai UniversitySchool of Pharmacy, Walailak UniversityDepartment of Biology, Faculty of Science, Chiang Mai UniversityResearch Institute for Health Sciences, Chiang Mai UniversityAbstract Objective Plants in the Annonaceae family are known for having abundant biologically active secondary metabolites. They have been used in alternative drugs for various diseases in several countries, for instance, the bark of Cananga odorata (Lam.) Hook and Thomson is used for Ophthalmic inflammation and wound healing in Malaysia. Extracts from the leaves and stems of four Annonaceae plants, namely Uvaria longipes (Craib) L.L.Zhou, Y.C.F.Su & R.M.K.Saunders, Dasymaschalon sp., Artabotrys burmanicus A.DC, and Marsypopetalum modestum (Pierre) B.Xue & R.M.K.Saunders were investigated for growth inhibitory activity against blood-stage Plasmodium falciparum growth in vitro and for non-specific cytotoxicity against normal peripheral blood mononuclear cells (PBMCs). Antimalarial activity was assessed by invasion inhibition assay and the percentage of infected red blood cells on blood smears were determined. Cytotoxicity was tested by culturing PBMCs with the extracts, and viabilities were determined by Annexin V/propidium iodide staining. Results A. burmanicus stem extract and M. modestum leaf extract were capable of inhibiting growth of P. falciparum when used at 200 µg/mL compared to chloroquine. The extracts at effective concentrations, did not affect the viability of PBMCs. These results support further need for characterization of active compounds from specific Annonaceae plants in order to exploit their components for potential malaria treatment.https://doi.org/10.1186/s13104-023-06664-wAnnonaceaeMalariaPlasmodium falciparumArtabotrys burmanicusMarsypopetalum modestum |
| spellingShingle | Pathrapol Lithanatudom Kriangkrai Chawansuntati Chalermpong Saenjum Tanawat Chaowasku Kritsadee Rattanathammethee Boonsong Wungsintaweekul Maslin Osathanunkul Jiraprapa Wipasa In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants BMC Research Notes Annonaceae Malaria Plasmodium falciparum Artabotrys burmanicus Marsypopetalum modestum |
| title | In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants |
| title_full | In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants |
| title_fullStr | In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants |
| title_full_unstemmed | In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants |
| title_short | In-vitro antimalarial activity of methanolic leaf- and stem-derived extracts from four Annonaceae plants |
| title_sort | in vitro antimalarial activity of methanolic leaf and stem derived extracts from four annonaceae plants |
| topic | Annonaceae Malaria Plasmodium falciparum Artabotrys burmanicus Marsypopetalum modestum |
| url | https://doi.org/10.1186/s13104-023-06664-w |
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