Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application
DNA is strongly adsorbed on oxidized graphene surfaces in the presence of divalent cations. Here, we studied the effect of DNA adsorption on electrochemical charge transfer at few-layered, oxygen-functionalized graphene (GO<sub>x</sub>) electrodes. DNA adsorption on the inkjet-printed GO...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/8/273 |
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| author | Narges Asefifeyzabadi Torrey E. Holland Poopalasingam Sivakumar Saikat Talapatra Ishani M. Senanayake Boyd M. Goodson Mohtashim H. Shamsi |
| author_facet | Narges Asefifeyzabadi Torrey E. Holland Poopalasingam Sivakumar Saikat Talapatra Ishani M. Senanayake Boyd M. Goodson Mohtashim H. Shamsi |
| author_sort | Narges Asefifeyzabadi |
| collection | DOAJ |
| description | DNA is strongly adsorbed on oxidized graphene surfaces in the presence of divalent cations. Here, we studied the effect of DNA adsorption on electrochemical charge transfer at few-layered, oxygen-functionalized graphene (GO<sub>x</sub>) electrodes. DNA adsorption on the inkjet-printed GO<sub>x</sub> electrodes caused amplified current response from ferro/ferricyanide redox probe at concentration range 1 aM–10 nM in differential pulse voltammetry. We studied a number of variables that may affect the current response of the interface: sequence type, conformation, concentration, length, and ionic strength. Later, we showed a proof-of-concept DNA biosensing application, which is free from chemical immobilization of the probe and sensitive at attomolar concentration regime. We propose that GO<sub>x</sub> electrodes promise a low-cost solution to fabricate a highly sensitive platform for label-free and chemisorption-free DNA biosensing. |
| first_indexed | 2024-03-10T08:58:06Z |
| format | Article |
| id | doaj.art-1112f0cd5a014091b54173760db8ce8c |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T08:58:06Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1112f0cd5a014091b54173760db8ce8c2023-11-22T06:57:41ZengMDPI AGBiosensors2079-63742021-08-0111827310.3390/bios11080273Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing ApplicationNarges Asefifeyzabadi0Torrey E. Holland1Poopalasingam Sivakumar2Saikat Talapatra3Ishani M. Senanayake4Boyd M. Goodson5Mohtashim H. Shamsi6School of Chemical and Biomolecular Sciences, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62918, USASchool of Physics and Applied Physics, Southern Illinois University, Carbondale, IL 62918, USASchool of Physics and Applied Physics, Southern Illinois University, Carbondale, IL 62918, USASchool of Physics and Applied Physics, Southern Illinois University, Carbondale, IL 62918, USASchool of Chemical and Biomolecular Sciences, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62918, USASchool of Chemical and Biomolecular Sciences, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62918, USASchool of Chemical and Biomolecular Sciences, Southern Illinois University, 1245 Lincoln Drive, Carbondale, IL 62918, USADNA is strongly adsorbed on oxidized graphene surfaces in the presence of divalent cations. Here, we studied the effect of DNA adsorption on electrochemical charge transfer at few-layered, oxygen-functionalized graphene (GO<sub>x</sub>) electrodes. DNA adsorption on the inkjet-printed GO<sub>x</sub> electrodes caused amplified current response from ferro/ferricyanide redox probe at concentration range 1 aM–10 nM in differential pulse voltammetry. We studied a number of variables that may affect the current response of the interface: sequence type, conformation, concentration, length, and ionic strength. Later, we showed a proof-of-concept DNA biosensing application, which is free from chemical immobilization of the probe and sensitive at attomolar concentration regime. We propose that GO<sub>x</sub> electrodes promise a low-cost solution to fabricate a highly sensitive platform for label-free and chemisorption-free DNA biosensing.https://www.mdpi.com/2079-6374/11/8/273DNA biosensorsgraphene electrodesinkjet-printingtrinucleotide repeatslabel-freeelectrochemical biosensors |
| spellingShingle | Narges Asefifeyzabadi Torrey E. Holland Poopalasingam Sivakumar Saikat Talapatra Ishani M. Senanayake Boyd M. Goodson Mohtashim H. Shamsi Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application Biosensors DNA biosensors graphene electrodes inkjet-printing trinucleotide repeats label-free electrochemical biosensors |
| title | Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application |
| title_full | Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application |
| title_fullStr | Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application |
| title_full_unstemmed | Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application |
| title_short | Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application |
| title_sort | sequence independent dna adsorption on few layered oxygen functionalized graphene electrodes an electrochemical study for biosensing application |
| topic | DNA biosensors graphene electrodes inkjet-printing trinucleotide repeats label-free electrochemical biosensors |
| url | https://www.mdpi.com/2079-6374/11/8/273 |
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