Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments
Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The applicati...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/10/352 |
| _version_ | 1797515120107061248 |
|---|---|
| author | Kuankuan Zhang Hua Zhang Haorui Cao Yu Jiang Kang Mao Zhugen Yang |
| author_facet | Kuankuan Zhang Hua Zhang Haorui Cao Yu Jiang Kang Mao Zhugen Yang |
| author_sort | Kuankuan Zhang |
| collection | DOAJ |
| description | Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted increasing attention; for example, rolling circle amplification (RCA) is an isothermal enzymatic process in which a short nucleic acid primer is amplified to form a long single-stranded nucleic acid using a circular template and special nucleic acid polymerases. Furthermore, this approach can be further engineered into a device for point-of-need monitoring of environmental pollutants. In this paper, we describe the fundamental principles of RCA and the advantages and disadvantages of RCA assays. Then, we discuss the recently developed RCA-based tools for environmental analysis to determine various targets, including heavy metals, organic small molecules, nucleic acids, peptides, proteins, and even microorganisms in aqueous environments. Finally, we summarize the challenges and outline strategies for the advancement of this technique for application in contaminant monitoring. |
| first_indexed | 2024-03-10T06:41:06Z |
| format | Article |
| id | doaj.art-1114d080246745a3bcbbf13d21307191 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T06:41:06Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-1114d080246745a3bcbbf13d213071912023-11-22T17:35:24ZengMDPI AGBiosensors2079-63742021-09-01111035210.3390/bios11100352Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous EnvironmentsKuankuan Zhang0Hua Zhang1Haorui Cao2Yu Jiang3Kang Mao4Zhugen Yang5State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, ChinaState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, ChinaState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, ChinaState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, ChinaState Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550081, ChinaCranfield Water Science Institute, Cranfield University, Cranfield MK43 0AL, UKEnvironmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted increasing attention; for example, rolling circle amplification (RCA) is an isothermal enzymatic process in which a short nucleic acid primer is amplified to form a long single-stranded nucleic acid using a circular template and special nucleic acid polymerases. Furthermore, this approach can be further engineered into a device for point-of-need monitoring of environmental pollutants. In this paper, we describe the fundamental principles of RCA and the advantages and disadvantages of RCA assays. Then, we discuss the recently developed RCA-based tools for environmental analysis to determine various targets, including heavy metals, organic small molecules, nucleic acids, peptides, proteins, and even microorganisms in aqueous environments. Finally, we summarize the challenges and outline strategies for the advancement of this technique for application in contaminant monitoring.https://www.mdpi.com/2079-6374/11/10/352rolling circle amplificationenvironmental monitoringheavy metalsorganic moleculesmicroorganisms |
| spellingShingle | Kuankuan Zhang Hua Zhang Haorui Cao Yu Jiang Kang Mao Zhugen Yang Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments Biosensors rolling circle amplification environmental monitoring heavy metals organic molecules microorganisms |
| title | Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments |
| title_full | Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments |
| title_fullStr | Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments |
| title_full_unstemmed | Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments |
| title_short | Rolling Circle Amplification as an Efficient Analytical Tool for Rapid Detection of Contaminants in Aqueous Environments |
| title_sort | rolling circle amplification as an efficient analytical tool for rapid detection of contaminants in aqueous environments |
| topic | rolling circle amplification environmental monitoring heavy metals organic molecules microorganisms |
| url | https://www.mdpi.com/2079-6374/11/10/352 |
| work_keys_str_mv | AT kuankuanzhang rollingcircleamplificationasanefficientanalyticaltoolforrapiddetectionofcontaminantsinaqueousenvironments AT huazhang rollingcircleamplificationasanefficientanalyticaltoolforrapiddetectionofcontaminantsinaqueousenvironments AT haoruicao rollingcircleamplificationasanefficientanalyticaltoolforrapiddetectionofcontaminantsinaqueousenvironments AT yujiang rollingcircleamplificationasanefficientanalyticaltoolforrapiddetectionofcontaminantsinaqueousenvironments AT kangmao rollingcircleamplificationasanefficientanalyticaltoolforrapiddetectionofcontaminantsinaqueousenvironments AT zhugenyang rollingcircleamplificationasanefficientanalyticaltoolforrapiddetectionofcontaminantsinaqueousenvironments |