Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)
The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or de...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00651/full |
| _version_ | 1811326123935531008 |
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| author | Kui eLin-Wang Tony eMcGhie Mindy eWang Yuhui eLiu Ben eWarren Roy eStorey Richard Victor Espley Andrew Charles Allan Andrew Charles Allan |
| author_facet | Kui eLin-Wang Tony eMcGhie Mindy eWang Yuhui eLiu Ben eWarren Roy eStorey Richard Victor Espley Andrew Charles Allan Andrew Charles Allan |
| author_sort | Kui eLin-Wang |
| collection | DOAJ |
| description | The woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the R2R3 MYB activator, MYB10. In contrast, a potential bHLH partner for MYB10 (bHLH33) did not affect the anthocyanin pathway when knocked down using RNAi constructs. Metabolic analysis of fruits revealed that, of all the polyphenolics surveyed, only cyanidin and pelargonidin glucoside, and coumaryl hexose were significantly affected by over-expression and knock down of MYB10. Using the F. vesca genome sequence, members of the MYB, bHLH and WD40 families were examined. Global analysis of gene expression and targeted qPCR analysis of biosynthetic genes and regulators confirmed the effects of altering MYB10 expression, as well as the knock-down of bHLH33. Other members of the MYB transcription factor family were affected by the transgenes. Transient expression of strawberry genes in Nicotiana benthamiana revealed that MYB10 can auto-regulate itself, and potential repressors of MYB10. In tobacco, MYB10’s activation of biosynthetic steps is inhibited by the strawberry repressor MYB1. |
| first_indexed | 2024-04-13T14:43:58Z |
| format | Article |
| id | doaj.art-111ac3eec2914828b8c2f2c8d2dcbcde |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T14:43:58Z |
| publishDate | 2014-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-111ac3eec2914828b8c2f2c8d2dcbcde2022-12-22T02:42:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2014-11-01510.3389/fpls.2014.00651110359Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca)Kui eLin-Wang0Tony eMcGhie1Mindy eWang2Yuhui eLiu3Ben eWarren4Roy eStorey5Richard Victor Espley6Andrew Charles Allan7Andrew Charles Allan8Plant & Food ResearchPlant and Food ResearchPlant & Food ResearchGansu Agricultural UniversityPlant & Food ResearchPlant & Food ResearchPlant & Food ResearchPlant & Food ResearchUniversity of AucklandThe woodland strawberry, Fragaria vesca is a model fruit for a number of rosaceous crops. We have engineered altered concentrations of anthocyanin in F. vesca, to determine the impact on plant growth and fruit quality. Anthocyanin concentrations were significantly increased by over-expression or decreased by knock-down of the R2R3 MYB activator, MYB10. In contrast, a potential bHLH partner for MYB10 (bHLH33) did not affect the anthocyanin pathway when knocked down using RNAi constructs. Metabolic analysis of fruits revealed that, of all the polyphenolics surveyed, only cyanidin and pelargonidin glucoside, and coumaryl hexose were significantly affected by over-expression and knock down of MYB10. Using the F. vesca genome sequence, members of the MYB, bHLH and WD40 families were examined. Global analysis of gene expression and targeted qPCR analysis of biosynthetic genes and regulators confirmed the effects of altering MYB10 expression, as well as the knock-down of bHLH33. Other members of the MYB transcription factor family were affected by the transgenes. Transient expression of strawberry genes in Nicotiana benthamiana revealed that MYB10 can auto-regulate itself, and potential repressors of MYB10. In tobacco, MYB10’s activation of biosynthetic steps is inhibited by the strawberry repressor MYB1.http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00651/fullregulationtranscription factorAnthocyaninFragaria vescaFvMYB10 |
| spellingShingle | Kui eLin-Wang Tony eMcGhie Mindy eWang Yuhui eLiu Ben eWarren Roy eStorey Richard Victor Espley Andrew Charles Allan Andrew Charles Allan Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) Frontiers in Plant Science regulation transcription factor Anthocyanin Fragaria vesca FvMYB10 |
| title | Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) |
| title_full | Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) |
| title_fullStr | Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) |
| title_full_unstemmed | Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) |
| title_short | Engineering the anthocyanin regulatory complex of strawberry (Fragaria vesca) |
| title_sort | engineering the anthocyanin regulatory complex of strawberry fragaria vesca |
| topic | regulation transcription factor Anthocyanin Fragaria vesca FvMYB10 |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2014.00651/full |
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