Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer
Objective MicroRNA (miR)-421 plays a key role in cancer progression. It has been reported that circulating miR-421may be a potential tumor marker for the diagnosis of several cancers. However, the role of miR-421 in plasma as a potential biomarker in the diagnosis of precancerous gastric lesions (Pr...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2019-06-01
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| Online Access: | https://peerj.com/articles/7002.pdf |
| _version_ | 1797419573093335040 |
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| author | Jianlin Chen Lihua Wu Yifan Sun Qi Yin Xianhua Chen Siqun Liang Qingyan Meng Haihua Long Fangying Li Changjun Luo Xiaorong Xiao |
| author_facet | Jianlin Chen Lihua Wu Yifan Sun Qi Yin Xianhua Chen Siqun Liang Qingyan Meng Haihua Long Fangying Li Changjun Luo Xiaorong Xiao |
| author_sort | Jianlin Chen |
| collection | DOAJ |
| description | Objective MicroRNA (miR)-421 plays a key role in cancer progression. It has been reported that circulating miR-421may be a potential tumor marker for the diagnosis of several cancers. However, the role of miR-421 in plasma as a potential biomarker in the diagnosis of precancerous gastric lesions (Pre) and early-stage gastric cancer (GC) remains poorly understood. In this study, we investigated miR-421 in plasma as a novel potential biomarker for the detection of precancerous gastric lesions and early-stage (GC). Materials & Methods The miRNA content was determined by quantitative real-time polymerase chain reaction (qRT-PCR). MiR-421 content in all subjects was normalized by endogenous miRNA (miR-16). The diagnostic value of miR-421 for Pre and GC was assessed by comparing receiver operating characteristic (ROC) analysis with traditional tumor markers, including CEA, CA125, CA153, CA211 and CA50. The correlation between the expression of miR-421 and the pathological characteristics of Pre and GC was analyzed. Results Elevated expression of miR-421 in plasma can robustly distinguish the normal population from Pre and GC cases, especially in the early stages of gastric cancer cases (all p < 0.05). The ROC analyses showed that the area under the ROC curve (AUC), sensitivity, accuracy and Youden index of miR-421 were superior to traditional tumor markers (CEA, CA125, CA153, CA211, and CA50) in GC diagnosis, while its specificity was higher than CEA, CA153 and CA50 (all p < 0.05). MiR-421 in plasma had higher AUC value than AFP, CA153, CA211 and CA50 in the diagnosis of Pre (all p < 0.05), while specificity, accuracy and Youden index of miR-421 was only lower than CA211. The efficiency of miR-421 in the diagnosis of GC was significantly higher than that of CA211 and CA50, and it was significantly higher than CA153, CA211 and CA50 in the diagnosis of Pre (all p < 0.05). In addition, up-regulation of miR-421 occurred initially in precancerous gastric lesions as well as in the early stage of GC. Conclusions Overexpression of plasma miR-421 is a novel biomarker for the detection of precancerous lesions and early gastric cancer. |
| first_indexed | 2024-03-09T06:48:54Z |
| format | Article |
| id | doaj.art-111ac51f3b9e46288e5cfe5d40eec21f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:48:54Z |
| publishDate | 2019-06-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-111ac51f3b9e46288e5cfe5d40eec21f2023-12-03T10:29:22ZengPeerJ Inc.PeerJ2167-83592019-06-017e700210.7717/peerj.7002Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancerJianlin Chen0Lihua Wu1Yifan Sun2Qi Yin3Xianhua Chen4Siqun Liang5Qingyan Meng6Haihua Long7Fangying Li8Changjun Luo9Xiaorong Xiao10Department of Clinical Laboratory, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Digestive Internal Medicine, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Clinical Laboratory, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Clinical Laboratory, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Clinical Laboratory, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Clinical Laboratory, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Clinical Laboratory, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Digestive Internal Medicine, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Digestive Internal Medicine, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Internal Medicine-Cardiovascular, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaDepartment of Science and Education, Affiliated Liutie Central Hospital of Guangxi Medical University, Liuzhou, Guangxi, ChinaObjective MicroRNA (miR)-421 plays a key role in cancer progression. It has been reported that circulating miR-421may be a potential tumor marker for the diagnosis of several cancers. However, the role of miR-421 in plasma as a potential biomarker in the diagnosis of precancerous gastric lesions (Pre) and early-stage gastric cancer (GC) remains poorly understood. In this study, we investigated miR-421 in plasma as a novel potential biomarker for the detection of precancerous gastric lesions and early-stage (GC). Materials & Methods The miRNA content was determined by quantitative real-time polymerase chain reaction (qRT-PCR). MiR-421 content in all subjects was normalized by endogenous miRNA (miR-16). The diagnostic value of miR-421 for Pre and GC was assessed by comparing receiver operating characteristic (ROC) analysis with traditional tumor markers, including CEA, CA125, CA153, CA211 and CA50. The correlation between the expression of miR-421 and the pathological characteristics of Pre and GC was analyzed. Results Elevated expression of miR-421 in plasma can robustly distinguish the normal population from Pre and GC cases, especially in the early stages of gastric cancer cases (all p < 0.05). The ROC analyses showed that the area under the ROC curve (AUC), sensitivity, accuracy and Youden index of miR-421 were superior to traditional tumor markers (CEA, CA125, CA153, CA211, and CA50) in GC diagnosis, while its specificity was higher than CEA, CA153 and CA50 (all p < 0.05). MiR-421 in plasma had higher AUC value than AFP, CA153, CA211 and CA50 in the diagnosis of Pre (all p < 0.05), while specificity, accuracy and Youden index of miR-421 was only lower than CA211. The efficiency of miR-421 in the diagnosis of GC was significantly higher than that of CA211 and CA50, and it was significantly higher than CA153, CA211 and CA50 in the diagnosis of Pre (all p < 0.05). In addition, up-regulation of miR-421 occurred initially in precancerous gastric lesions as well as in the early stage of GC. Conclusions Overexpression of plasma miR-421 is a novel biomarker for the detection of precancerous lesions and early gastric cancer.https://peerj.com/articles/7002.pdfEarly diagnosisPrecancerous lesionsMicroRNA-421Gastric cancer |
| spellingShingle | Jianlin Chen Lihua Wu Yifan Sun Qi Yin Xianhua Chen Siqun Liang Qingyan Meng Haihua Long Fangying Li Changjun Luo Xiaorong Xiao Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer PeerJ Early diagnosis Precancerous lesions MicroRNA-421 Gastric cancer |
| title | Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer |
| title_full | Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer |
| title_fullStr | Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer |
| title_full_unstemmed | Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer |
| title_short | Mir-421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer |
| title_sort | mir 421 in plasma as a potential diagnostic biomarker for precancerous gastric lesions and early gastric cancer |
| topic | Early diagnosis Precancerous lesions MicroRNA-421 Gastric cancer |
| url | https://peerj.com/articles/7002.pdf |
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