Mosquito microbiota cluster by host sampling location
Abstract Background Microbial communities that inhabit the mosquito body play an import role in host biology and may have potential for mosquito control. However, the forces that shape these microbial communities are poorly understood. Methods To gain a better understanding of how host location infl...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-018-3036-9 |
| _version_ | 1818364531118702592 |
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| author | Ephantus J. Muturi Doris Lagos-Kutz Christopher Dunlap Jose L. Ramirez Alejandro P. Rooney Glen L. Hartman Christopher J. Fields Gloria Rendon Chang-Hyun Kim |
| author_facet | Ephantus J. Muturi Doris Lagos-Kutz Christopher Dunlap Jose L. Ramirez Alejandro P. Rooney Glen L. Hartman Christopher J. Fields Gloria Rendon Chang-Hyun Kim |
| author_sort | Ephantus J. Muturi |
| collection | DOAJ |
| description | Abstract Background Microbial communities that inhabit the mosquito body play an import role in host biology and may have potential for mosquito control. However, the forces that shape these microbial communities are poorly understood. Methods To gain a better understanding of how host location influences the composition and diversity of mosquito microbiota, we performed a survey of microbial communities in mosquito samples collected from six USA states using HiSeq sequencing of the 16S rRNA gene. Results A total of 284 bacterial operational taxonomic units (OTUs) belonging to 14 phyla were detected in nine mosquito species, with Proteobacteria, Firmicutes and Actinobacteria accounting for 95% of total sequences. OTU richness varied markedly within and between mosquito species. The microbial composition and diversity was heavily influenced by the site of mosquito collection, suggesting that host location plays an important role in shaping the mosquito microbiota. Conclusions Variation in microbial composition and diversity between mosquitoes from different locations may have important implications on vector competence and transmission dynamics of mosquito-borne pathogens. Future studies should investigate the environmental factors responsible for these variations and the role of key bacteria characterized in this study on mosquito biology and their potential application in symbiotic control of mosquito-borne diseases. |
| first_indexed | 2024-12-13T22:05:51Z |
| format | Article |
| id | doaj.art-111c293aa97643a0a44fd343d4c7c49e |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-13T22:05:51Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-111c293aa97643a0a44fd343d4c7c49e2022-12-21T23:29:50ZengBMCParasites & Vectors1756-33052018-08-0111111210.1186/s13071-018-3036-9Mosquito microbiota cluster by host sampling locationEphantus J. Muturi0Doris Lagos-Kutz1Christopher Dunlap2Jose L. Ramirez3Alejandro P. Rooney4Glen L. Hartman5Christopher J. Fields6Gloria Rendon7Chang-Hyun Kim8Crop Bioprotection Research Unit, Agricultural Research Service, U.S. Department of AgricultureNational Soybean Research Center, Agricultural Research Service,U.S. Department of AgricultureCrop Bioprotection Research Unit, Agricultural Research Service, U.S. Department of AgricultureCrop Bioprotection Research Unit, Agricultural Research Service, U.S. Department of AgricultureCrop Bioprotection Research Unit, Agricultural Research Service, U.S. Department of AgricultureNational Soybean Research Center, Agricultural Research Service,U.S. Department of AgricultureHigh Performance Biological Computing (HPCBio), Roy J Carver Biotechnology Center, University of Illinois at Urbana-ChampaignHigh Performance Biological Computing (HPCBio), Roy J Carver Biotechnology Center, University of Illinois at Urbana-ChampaignIllinois Natural History Survey, University of Illinois at Urbana-ChampaignAbstract Background Microbial communities that inhabit the mosquito body play an import role in host biology and may have potential for mosquito control. However, the forces that shape these microbial communities are poorly understood. Methods To gain a better understanding of how host location influences the composition and diversity of mosquito microbiota, we performed a survey of microbial communities in mosquito samples collected from six USA states using HiSeq sequencing of the 16S rRNA gene. Results A total of 284 bacterial operational taxonomic units (OTUs) belonging to 14 phyla were detected in nine mosquito species, with Proteobacteria, Firmicutes and Actinobacteria accounting for 95% of total sequences. OTU richness varied markedly within and between mosquito species. The microbial composition and diversity was heavily influenced by the site of mosquito collection, suggesting that host location plays an important role in shaping the mosquito microbiota. Conclusions Variation in microbial composition and diversity between mosquitoes from different locations may have important implications on vector competence and transmission dynamics of mosquito-borne pathogens. Future studies should investigate the environmental factors responsible for these variations and the role of key bacteria characterized in this study on mosquito biology and their potential application in symbiotic control of mosquito-borne diseases.http://link.springer.com/article/10.1186/s13071-018-3036-9MosquitoesMicrobiotaHiSeq16S rRNA gene |
| spellingShingle | Ephantus J. Muturi Doris Lagos-Kutz Christopher Dunlap Jose L. Ramirez Alejandro P. Rooney Glen L. Hartman Christopher J. Fields Gloria Rendon Chang-Hyun Kim Mosquito microbiota cluster by host sampling location Parasites & Vectors Mosquitoes Microbiota HiSeq 16S rRNA gene |
| title | Mosquito microbiota cluster by host sampling location |
| title_full | Mosquito microbiota cluster by host sampling location |
| title_fullStr | Mosquito microbiota cluster by host sampling location |
| title_full_unstemmed | Mosquito microbiota cluster by host sampling location |
| title_short | Mosquito microbiota cluster by host sampling location |
| title_sort | mosquito microbiota cluster by host sampling location |
| topic | Mosquitoes Microbiota HiSeq 16S rRNA gene |
| url | http://link.springer.com/article/10.1186/s13071-018-3036-9 |
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