Identification of beef using restriction fragment length polymorphism–
To differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to obtain amplified band 359 bp, then digested w...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2009-01-01
|
| Series: | Iraqi Journal of Veterinary Sciences |
| Online Access: | http://www.vetmedmosul.org/ijvs/media/9-1-8a.pdf |
| _version_ | 1811248981125103616 |
|---|---|
| author | R. A. Al-Sanjary |
| author_facet | R. A. Al-Sanjary |
| author_sort | R. A. Al-Sanjary |
| collection | DOAJ |
| description | To differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to obtain amplified band 359 bp, then digested with some of restriction enzymes like Tru91, RsaI, Hinf I, Hae III, Alu I, Taq I, Mob I. The result revealed that, the Hinf I enzyme produce three bands 198, 117, 44 bp and the Hae III enzyme revealed two band 285, 74 bp, the Alu I enzyme also produced two band but the molecular weight are 190, 169 bp. The other enzymes did not reveal any digestion of the amplified bands and this result is a characteristic unique to beef compared with other types of meat when using same enzymes. |
| first_indexed | 2024-04-12T15:38:26Z |
| format | Article |
| id | doaj.art-111d96ae49a74ff89cbe5b339f1cd385 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-12T15:38:26Z |
| publishDate | 2009-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-111d96ae49a74ff89cbe5b339f1cd3852022-12-22T03:26:53ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552009-01-0123 (Arabic)14346Identification of beef using restriction fragment length polymorphism–R. A. Al-SanjaryTo differentiate the beef from other types of meat consumed by human, DNA markers based on polymerase chain reaction restriction fragment length polymorphism technique is performed by using universal primers designed on mitochondrial cytochrome b gene to obtain amplified band 359 bp, then digested with some of restriction enzymes like Tru91, RsaI, Hinf I, Hae III, Alu I, Taq I, Mob I. The result revealed that, the Hinf I enzyme produce three bands 198, 117, 44 bp and the Hae III enzyme revealed two band 285, 74 bp, the Alu I enzyme also produced two band but the molecular weight are 190, 169 bp. The other enzymes did not reveal any digestion of the amplified bands and this result is a characteristic unique to beef compared with other types of meat when using same enzymes.http://www.vetmedmosul.org/ijvs/media/9-1-8a.pdf |
| spellingShingle | R. A. Al-Sanjary Identification of beef using restriction fragment length polymorphism– Iraqi Journal of Veterinary Sciences |
| title | Identification of beef using restriction fragment length polymorphism– |
| title_full | Identification of beef using restriction fragment length polymorphism– |
| title_fullStr | Identification of beef using restriction fragment length polymorphism– |
| title_full_unstemmed | Identification of beef using restriction fragment length polymorphism– |
| title_short | Identification of beef using restriction fragment length polymorphism– |
| title_sort | identification of beef using restriction fragment length polymorphism |
| url | http://www.vetmedmosul.org/ijvs/media/9-1-8a.pdf |
| work_keys_str_mv | AT raalsanjary identificationofbeefusingrestrictionfragmentlengthpolymorphism |