Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations
The vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/8/2609 |
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| author | Michal Ješeta Andrea Celá Jana Žáková Aleš Mádr Igor Crha Zdeněk Glatz Bartosz Kempisty Pavel Ventruba |
| author_facet | Michal Ješeta Andrea Celá Jana Žáková Aleš Mádr Igor Crha Zdeněk Glatz Bartosz Kempisty Pavel Ventruba |
| author_sort | Michal Ješeta |
| collection | DOAJ |
| description | The vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the warmed embryos was observed in the context of a cultivation environment, which was with the atmospheric oxygen concentration ≈20% or with a regulated oxygen level—hysiological (5%). It is the first time that total amino acid turnover in human embryos after their freezing at post compaction stages has been evaluated. During this study, progressive embryos (developed to blastocyst stage) and stagnant embryos (without developmental progression) were analyzed. It was observed that the embryos cultivated in conditions of physiological oxygen levels (5% oxygen) showed a significantly lower consumption of amino acids from the cultivation media. Progressively developing embryos also had significantly lower total amino acid turnovers (consumption and production of amino acids) when cultured in conditions with physiological oxygen levels. Based on these results it seems that a cultivation environment with a reduced oxygen concentration decreases the risk of degenerative changes in the embryos after thawing. Therefore, the cultivation of thawed embryos in an environment with physiological oxygen levels may preclude embryonal stagnation, and can support the further development of human embryos after their thawing. |
| first_indexed | 2024-03-10T17:35:11Z |
| format | Article |
| id | doaj.art-1121dc73136b4a58a5c9cebc94b62b6b |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T17:35:11Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-1121dc73136b4a58a5c9cebc94b62b6b2023-11-20T09:51:37ZengMDPI AGJournal of Clinical Medicine2077-03832020-08-0198260910.3390/jcm9082609Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen ConcentrationsMichal Ješeta0Andrea Celá1Jana Žáková2Aleš Mádr3Igor Crha4Zdeněk Glatz5Bartosz Kempisty6Pavel Ventruba7Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, 602 00 Brno, Czech RepublicDepartment of Biochemistry, Faculty of Science, Masaryk University, 602 00 Brno, Czech RepublicDepartment of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, 602 00 Brno, Czech RepublicDepartment of Biochemistry, Faculty of Science, Masaryk University, 602 00 Brno, Czech RepublicDepartment of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, 602 00 Brno, Czech RepublicDepartment of Biochemistry, Faculty of Science, Masaryk University, 602 00 Brno, Czech RepublicDepartment of Veterinary Surgery, Nicolaus Copernicus University, 87 100 Torun, PolandDepartment of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno, 602 00 Brno, Czech RepublicThe vitrification of human embryos is more and more frequently being utilized as a method of assisted reproduction. For this technique, gentle treatment of the embryos after thawing is crucial. In this study, the balance of amino acids released to/consumed from the cultivation media surrounding the warmed embryos was observed in the context of a cultivation environment, which was with the atmospheric oxygen concentration ≈20% or with a regulated oxygen level—hysiological (5%). It is the first time that total amino acid turnover in human embryos after their freezing at post compaction stages has been evaluated. During this study, progressive embryos (developed to blastocyst stage) and stagnant embryos (without developmental progression) were analyzed. It was observed that the embryos cultivated in conditions of physiological oxygen levels (5% oxygen) showed a significantly lower consumption of amino acids from the cultivation media. Progressively developing embryos also had significantly lower total amino acid turnovers (consumption and production of amino acids) when cultured in conditions with physiological oxygen levels. Based on these results it seems that a cultivation environment with a reduced oxygen concentration decreases the risk of degenerative changes in the embryos after thawing. Therefore, the cultivation of thawed embryos in an environment with physiological oxygen levels may preclude embryonal stagnation, and can support the further development of human embryos after their thawing.https://www.mdpi.com/2077-0383/9/8/2609amino acidshuman embryoin vitro cultivationcapillary electrophoresisfluorescence detectionmetabolic activity |
| spellingShingle | Michal Ješeta Andrea Celá Jana Žáková Aleš Mádr Igor Crha Zdeněk Glatz Bartosz Kempisty Pavel Ventruba Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations Journal of Clinical Medicine amino acids human embryo in vitro cultivation capillary electrophoresis fluorescence detection metabolic activity |
| title | Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations |
| title_full | Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations |
| title_fullStr | Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations |
| title_full_unstemmed | Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations |
| title_short | Metabolic Activity of Human Embryos after Thawing Differs in Atmosphere with Different Oxygen Concentrations |
| title_sort | metabolic activity of human embryos after thawing differs in atmosphere with different oxygen concentrations |
| topic | amino acids human embryo in vitro cultivation capillary electrophoresis fluorescence detection metabolic activity |
| url | https://www.mdpi.com/2077-0383/9/8/2609 |
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