Optimization of levan production by Zymomonas mobilis
Effect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l). Above 15% (20 and 25%) sucrose concentration, there was no increase in the biom...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
1999-01-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300004 |
| _version_ | 1818951574690463744 |
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| author | V. K Ananthalakshmy P Gunasekaran |
| author_facet | V. K Ananthalakshmy P Gunasekaran |
| author_sort | V. K Ananthalakshmy |
| collection | DOAJ |
| description | Effect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l). Above 15% (20 and 25%) sucrose concentration, there was no increase in the biomass. The sucrose hydrolysis and levan production occurred even in the absence of significant growth of cells. Maximum amount of levan was produced (14.5 g/l) at pH 5 and 15 g /l at 25(0)C temperature. At temperature between 35(0)C and 40(0)C, levan production was not detected. Presence of glucose in the medium considerably reduced levan production (2.8 g/l) than fructose 6.7g/l.<br>O efeito de diferentes condições de fermentação na produção de levan por Zymomonas mobilis B-4286 foi estudado. A produção de Levan aumentou de 5.7-g/l a 12.6-g/l com o aumento da concentração inicial de sacarose (50-150 g/l). Acima de 15%, 20 e 25% a concentração de sacarose, não propiciou nenhum acréscimo na formação de biomassa. A hidrólise da sacarose e produção de Levan ocorreram de forma normal na ausência de um crescimento celular significativo. A concentração máxima de levan produzida foi (14.5 g/l) em pH 5, 15 g /l a 25(0) C. Na temperatura entre 35(0)C e 40(0) C, não ocorreu a produção de levan. A presença de glicose no meio de cultivo reduziu consideravelmente a produção média de levan (2.8 g/l) bem como a de frutose (6.7g/l.). |
| first_indexed | 2024-12-20T09:36:39Z |
| format | Article |
| id | doaj.art-112208a2921346648b4317de4cae8ed3 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-20T09:36:39Z |
| publishDate | 1999-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-112208a2921346648b4317de4cae8ed32022-12-21T19:44:58ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43241999-01-0142329129810.1590/S1516-89131999000300004Optimization of levan production by Zymomonas mobilisV. K AnanthalakshmyP GunasekaranEffect of different fermentation conditions on levan production by Zymomonas mobilis B-4286 was studied. Levan production increased from 5.7-g/l to 12.6-g/l with an increase in initial sucrose concentration (50-150 g/l). Above 15% (20 and 25%) sucrose concentration, there was no increase in the biomass. The sucrose hydrolysis and levan production occurred even in the absence of significant growth of cells. Maximum amount of levan was produced (14.5 g/l) at pH 5 and 15 g /l at 25(0)C temperature. At temperature between 35(0)C and 40(0)C, levan production was not detected. Presence of glucose in the medium considerably reduced levan production (2.8 g/l) than fructose 6.7g/l.<br>O efeito de diferentes condições de fermentação na produção de levan por Zymomonas mobilis B-4286 foi estudado. A produção de Levan aumentou de 5.7-g/l a 12.6-g/l com o aumento da concentração inicial de sacarose (50-150 g/l). Acima de 15%, 20 e 25% a concentração de sacarose, não propiciou nenhum acréscimo na formação de biomassa. A hidrólise da sacarose e produção de Levan ocorreram de forma normal na ausência de um crescimento celular significativo. A concentração máxima de levan produzida foi (14.5 g/l) em pH 5, 15 g /l a 25(0) C. Na temperatura entre 35(0)C e 40(0) C, não ocorreu a produção de levan. A presença de glicose no meio de cultivo reduziu consideravelmente a produção média de levan (2.8 g/l) bem como a de frutose (6.7g/l.).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300004LevanLevansucraseSucraseZymomonas mobilis |
| spellingShingle | V. K Ananthalakshmy P Gunasekaran Optimization of levan production by Zymomonas mobilis Brazilian Archives of Biology and Technology Levan Levansucrase Sucrase Zymomonas mobilis |
| title | Optimization of levan production by Zymomonas mobilis |
| title_full | Optimization of levan production by Zymomonas mobilis |
| title_fullStr | Optimization of levan production by Zymomonas mobilis |
| title_full_unstemmed | Optimization of levan production by Zymomonas mobilis |
| title_short | Optimization of levan production by Zymomonas mobilis |
| title_sort | optimization of levan production by zymomonas mobilis |
| topic | Levan Levansucrase Sucrase Zymomonas mobilis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300004 |
| work_keys_str_mv | AT vkananthalakshmy optimizationoflevanproductionbyzymomonasmobilis AT pgunasekaran optimizationoflevanproductionbyzymomonasmobilis |