Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells
Low remyelination efficiency after spinal cord injury (SCI) is a major restraint to successful axonal and functional regeneration in mammals. In contrast, adult zebrafish can: (i) regenerate oligodendrocytes and myelin sheaths within 2 weeks post lesion; (ii) re-grow axonal projections across the le...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2019.00102/full |
| _version_ | 1818590153664364544 |
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| author | Vasiliki Tsata Volker Kroehne Susanne Reinhardt Ali El-Armouche Michael Brand Michael Wagner Michael Wagner Michell M. Reimer |
| author_facet | Vasiliki Tsata Volker Kroehne Susanne Reinhardt Ali El-Armouche Michael Brand Michael Wagner Michael Wagner Michell M. Reimer |
| author_sort | Vasiliki Tsata |
| collection | DOAJ |
| description | Low remyelination efficiency after spinal cord injury (SCI) is a major restraint to successful axonal and functional regeneration in mammals. In contrast, adult zebrafish can: (i) regenerate oligodendrocytes and myelin sheaths within 2 weeks post lesion; (ii) re-grow axonal projections across the lesion site and (iii) recover locomotor function within 6 weeks after spinal cord transection. However, little is known about the intrinsic properties of oligodendrocyte progenitor cells (OPCs), the remyelinating cells of the central nervous system (CNS). Here, we demonstrate that purified OPCs from the adult zebrafish spinal cord are electrically active. They functionally express voltage-gated K+ and Na+ channels, glutamate receptors and exhibit depolarizing, tetrodotoxin (TTX)-sensitive spikes, as previously seen in rodent and human OPCs. Furthermore, we show that the percentage of zebrafish OPCs exhibiting depolarizing spikes and Nav-mediated currents is lower as compared to rodent white matter OPCs, where these membrane characteristics have been shown to underlie OPC injury susceptibility. These findings imply that adult zebrafish OPCs resemble electrical properties found in mammals and represent a relevant cell type towards understanding the biology of the primary cells targeted in remyelination therapies for non-regenerative species. The in vitro platform introduced in this study could be used in the future to: (i) elucidate how membrane characteristics of zebrafish OPCs change upon injury and (ii) identify potential signaling components underlying OPC injury recognition. |
| first_indexed | 2024-12-16T09:52:01Z |
| format | Article |
| id | doaj.art-1122657f38e2401c8eb874a7d877775b |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-16T09:52:01Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-1122657f38e2401c8eb874a7d877775b2022-12-21T22:36:02ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022019-04-011310.3389/fncel.2019.00102433310Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor CellsVasiliki Tsata0Volker Kroehne1Susanne Reinhardt2Ali El-Armouche3Michael Brand4Michael Wagner5Michael Wagner6Michell M. Reimer7Center for Regenerative Therapies TU Dresden (CRTD) and Center for Molecular and Cellular Bioengineering (CMCB), Technische Universitaet, Dresden, GermanyCenter for Regenerative Therapies TU Dresden (CRTD) and Center for Molecular and Cellular Bioengineering (CMCB), Technische Universitaet, Dresden, GermanyDresden Genome Center, Center for Regenerative Therapies TU Dresden (CRTD), Center for Molecular and Cellular Bioengineering (CMCB), Technische Universitaet Dresden, Dresden, GermanyDepartment of Pharmacology and Toxicology, Technische Universitaet Dresden, Dresden, GermanyCenter for Regenerative Therapies TU Dresden (CRTD) and Center for Molecular and Cellular Bioengineering (CMCB), Technische Universitaet, Dresden, GermanyDepartment of Pharmacology and Toxicology, Technische Universitaet Dresden, Dresden, GermanyDepartment of Rhythmology, Heart Center Dresden, Technische Universitaet Dresden, Dresden, GermanyCenter for Regenerative Therapies TU Dresden (CRTD) and Center for Molecular and Cellular Bioengineering (CMCB), Technische Universitaet, Dresden, GermanyLow remyelination efficiency after spinal cord injury (SCI) is a major restraint to successful axonal and functional regeneration in mammals. In contrast, adult zebrafish can: (i) regenerate oligodendrocytes and myelin sheaths within 2 weeks post lesion; (ii) re-grow axonal projections across the lesion site and (iii) recover locomotor function within 6 weeks after spinal cord transection. However, little is known about the intrinsic properties of oligodendrocyte progenitor cells (OPCs), the remyelinating cells of the central nervous system (CNS). Here, we demonstrate that purified OPCs from the adult zebrafish spinal cord are electrically active. They functionally express voltage-gated K+ and Na+ channels, glutamate receptors and exhibit depolarizing, tetrodotoxin (TTX)-sensitive spikes, as previously seen in rodent and human OPCs. Furthermore, we show that the percentage of zebrafish OPCs exhibiting depolarizing spikes and Nav-mediated currents is lower as compared to rodent white matter OPCs, where these membrane characteristics have been shown to underlie OPC injury susceptibility. These findings imply that adult zebrafish OPCs resemble electrical properties found in mammals and represent a relevant cell type towards understanding the biology of the primary cells targeted in remyelination therapies for non-regenerative species. The in vitro platform introduced in this study could be used in the future to: (i) elucidate how membrane characteristics of zebrafish OPCs change upon injury and (ii) identify potential signaling components underlying OPC injury recognition.https://www.frontiersin.org/article/10.3389/fncel.2019.00102/fulladult zebrafishspinal cordoligodendrocyte progenitor cellsin vitroelectrophysiologypatch-clamp |
| spellingShingle | Vasiliki Tsata Volker Kroehne Susanne Reinhardt Ali El-Armouche Michael Brand Michael Wagner Michael Wagner Michell M. Reimer Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells Frontiers in Cellular Neuroscience adult zebrafish spinal cord oligodendrocyte progenitor cells in vitro electrophysiology patch-clamp |
| title | Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells |
| title_full | Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells |
| title_fullStr | Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells |
| title_full_unstemmed | Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells |
| title_short | Electrophysiological Properties of Adult Zebrafish Oligodendrocyte Progenitor Cells |
| title_sort | electrophysiological properties of adult zebrafish oligodendrocyte progenitor cells |
| topic | adult zebrafish spinal cord oligodendrocyte progenitor cells in vitro electrophysiology patch-clamp |
| url | https://www.frontiersin.org/article/10.3389/fncel.2019.00102/full |
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