Ultrastructure expansion microscopy in Trypanosoma brucei
The recently developed ultrastructure expansion microscopy (U-ExM) technique allows us to increase the spatial resolution within a cell or tissue for microscopic imaging through the physical expansion of the sample. In this study, we validate the use of U-ExM in Trypanosoma brucei measuring the expa...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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The Royal Society
2021-10-01
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| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.210132 |
| _version_ | 1797785629501685760 |
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| author | Ana Kalichava Torsten Ochsenreiter |
| author_facet | Ana Kalichava Torsten Ochsenreiter |
| author_sort | Ana Kalichava |
| collection | DOAJ |
| description | The recently developed ultrastructure expansion microscopy (U-ExM) technique allows us to increase the spatial resolution within a cell or tissue for microscopic imaging through the physical expansion of the sample. In this study, we validate the use of U-ExM in Trypanosoma brucei measuring the expansion factors of several different compartments/organelles and thus verify the isotropic expansion of the cell. We furthermore demonstrate the use of this sample preparation protocol for future studies by visualizing the nucleus and kDNA, as well as proteins of the cytoskeleton, the basal body, the mitochondrion and the endoplasmic reticulum. Lastly, we discuss the challenges and opportunities of U-ExM. |
| first_indexed | 2024-03-13T00:56:44Z |
| format | Article |
| id | doaj.art-1123ff0fb8bb4be584919ba87ee00d77 |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-03-13T00:56:44Z |
| publishDate | 2021-10-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-1123ff0fb8bb4be584919ba87ee00d772023-07-06T16:15:24ZengThe Royal SocietyOpen Biology2046-24412021-10-01111010.1098/rsob.210132Ultrastructure expansion microscopy in Trypanosoma bruceiAna Kalichava0Torsten Ochsenreiter1Institute of Cell Biology, University of Bern, SwitzerlandInstitute of Cell Biology, University of Bern, SwitzerlandThe recently developed ultrastructure expansion microscopy (U-ExM) technique allows us to increase the spatial resolution within a cell or tissue for microscopic imaging through the physical expansion of the sample. In this study, we validate the use of U-ExM in Trypanosoma brucei measuring the expansion factors of several different compartments/organelles and thus verify the isotropic expansion of the cell. We furthermore demonstrate the use of this sample preparation protocol for future studies by visualizing the nucleus and kDNA, as well as proteins of the cytoskeleton, the basal body, the mitochondrion and the endoplasmic reticulum. Lastly, we discuss the challenges and opportunities of U-ExM.https://royalsocietypublishing.org/doi/10.1098/rsob.210132T. bruceiexpansion microscopycentriolekDNAcell structuresuper resolution |
| spellingShingle | Ana Kalichava Torsten Ochsenreiter Ultrastructure expansion microscopy in Trypanosoma brucei Open Biology T. brucei expansion microscopy centriole kDNA cell structure super resolution |
| title | Ultrastructure expansion microscopy in Trypanosoma brucei |
| title_full | Ultrastructure expansion microscopy in Trypanosoma brucei |
| title_fullStr | Ultrastructure expansion microscopy in Trypanosoma brucei |
| title_full_unstemmed | Ultrastructure expansion microscopy in Trypanosoma brucei |
| title_short | Ultrastructure expansion microscopy in Trypanosoma brucei |
| title_sort | ultrastructure expansion microscopy in trypanosoma brucei |
| topic | T. brucei expansion microscopy centriole kDNA cell structure super resolution |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.210132 |
| work_keys_str_mv | AT anakalichava ultrastructureexpansionmicroscopyintrypanosomabrucei AT torstenochsenreiter ultrastructureexpansionmicroscopyintrypanosomabrucei |