Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i
As environmental opportunistic pathogens, nontuberculous mycobacteria (NTM) can cause severe and difficult to treat pulmonary disease. In the United States, Hawai’i has the highest prevalence of infection. Rapid growing mycobacteria (RGM) such as Mycobacterium abscessus and M. porcinum and the slow...
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| Format: | Article |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1268963/full |
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| author | Cara G. Tan Nicole M. Oberlag Acelyn E. McGowan Stephanie N. Dawrs Yvonne L. Chan Michael Strong Nabeeh A. Hasan Jennifer R. Honda Jennifer R. Honda |
| author_facet | Cara G. Tan Nicole M. Oberlag Acelyn E. McGowan Stephanie N. Dawrs Yvonne L. Chan Michael Strong Nabeeh A. Hasan Jennifer R. Honda Jennifer R. Honda |
| author_sort | Cara G. Tan |
| collection | DOAJ |
| description | As environmental opportunistic pathogens, nontuberculous mycobacteria (NTM) can cause severe and difficult to treat pulmonary disease. In the United States, Hawai’i has the highest prevalence of infection. Rapid growing mycobacteria (RGM) such as Mycobacterium abscessus and M. porcinum and the slow growing mycobacteria (SGM) including M. intracellulare subspecies chimaera are common environmental NTM species and subspecies in Hawai’i. Although iron acquisition is an essential process of many microorganisms, iron acquisition via siderophores among the NTM is not well-characterized. In this study, we apply genomic and microbiological methodologies to better understand iron acquisition via siderophores for environmental and respiratory isolates of M. abscessus, M. porcinum, and M. intracellulare subspecies chimaera from Hawai’i. Siderophore synthesis and transport genes, including mycobactin (mbt), mmpL/S, and esx-3 were compared among 47 reference isolates, 29 respiratory isolates, and 23 environmental Hawai’i isolates. Among all reference isolates examined, respiratory isolates showed significantly more siderophore pertinent genes compared to environmental isolates. Among the Hawai’i isolates, RGM M. abscessus and M. porcinum had significantly less esx-3 and mbt genes compared to SGM M. chimaera when stratified by growth classification. However, no significant differences were observed between the species when grown on low iron culture agar or siderophore production by the chrome azurol S (CAS) assay in vitro. These results indicate the complex mechanisms involved in iron sequestration and siderophore activity among diverse NTM species. |
| first_indexed | 2024-03-11T10:53:45Z |
| format | Article |
| id | doaj.art-112645419f6f41bead4244db9ec78369 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T10:53:45Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-112645419f6f41bead4244db9ec783692023-11-13T11:49:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-11-011410.3389/fmicb.2023.12689631268963Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’iCara G. Tan0Nicole M. Oberlag1Acelyn E. McGowan2Stephanie N. Dawrs3Yvonne L. Chan4Michael Strong5Nabeeh A. Hasan6Jennifer R. Honda7Jennifer R. Honda8‘Iolani School, Honolulu, HI, United StatesCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, United StatesRock Canyon High School, Highlands Ranch, CO, United StatesCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, United States‘Iolani School, Honolulu, HI, United StatesCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, United StatesCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, United StatesCenter for Genes, Environment and Health, National Jewish Health, Denver, CO, United StatesDepartment of Cellular and Molecular Biology, School of Medicine, The University of Texas Health Science Center at Tyler, Tyler, TX, United StatesAs environmental opportunistic pathogens, nontuberculous mycobacteria (NTM) can cause severe and difficult to treat pulmonary disease. In the United States, Hawai’i has the highest prevalence of infection. Rapid growing mycobacteria (RGM) such as Mycobacterium abscessus and M. porcinum and the slow growing mycobacteria (SGM) including M. intracellulare subspecies chimaera are common environmental NTM species and subspecies in Hawai’i. Although iron acquisition is an essential process of many microorganisms, iron acquisition via siderophores among the NTM is not well-characterized. In this study, we apply genomic and microbiological methodologies to better understand iron acquisition via siderophores for environmental and respiratory isolates of M. abscessus, M. porcinum, and M. intracellulare subspecies chimaera from Hawai’i. Siderophore synthesis and transport genes, including mycobactin (mbt), mmpL/S, and esx-3 were compared among 47 reference isolates, 29 respiratory isolates, and 23 environmental Hawai’i isolates. Among all reference isolates examined, respiratory isolates showed significantly more siderophore pertinent genes compared to environmental isolates. Among the Hawai’i isolates, RGM M. abscessus and M. porcinum had significantly less esx-3 and mbt genes compared to SGM M. chimaera when stratified by growth classification. However, no significant differences were observed between the species when grown on low iron culture agar or siderophore production by the chrome azurol S (CAS) assay in vitro. These results indicate the complex mechanisms involved in iron sequestration and siderophore activity among diverse NTM species.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1268963/fullnontuberculous mycobacteriaHawai’isiderophoresbioinformaticsCAS assay |
| spellingShingle | Cara G. Tan Nicole M. Oberlag Acelyn E. McGowan Stephanie N. Dawrs Yvonne L. Chan Michael Strong Nabeeh A. Hasan Jennifer R. Honda Jennifer R. Honda Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i Frontiers in Microbiology nontuberculous mycobacteria Hawai’i siderophores bioinformatics CAS assay |
| title | Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i |
| title_full | Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i |
| title_fullStr | Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i |
| title_full_unstemmed | Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i |
| title_short | Genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from Hawai’i |
| title_sort | genomic and microbiological analyses of iron acquisition pathways among respiratory and environmental nontuberculous mycobacteria from hawai i |
| topic | nontuberculous mycobacteria Hawai’i siderophores bioinformatics CAS assay |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1268963/full |
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