Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides
Polyetheretherketone (PEEK) is a thermoplastic polymer that has been recently employed for bone tissue engineering as a result of its biocompatibility and mechanical properties being comparable to human bone. PEEK, however, is a bio-inert material and, when implanted, does not interact with the host...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/2/246 |
| _version_ | 1797622109709533184 |
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| author | Leonardo Cassari Annj Zamuner Grazia M. L. Messina Martina Marsotto Hongyi Chen Giovanni Gonnella Trevor Coward Chiara Battocchio Jie Huang Giovanna Iucci Giovanni Marletta Lucy Di Silvio Monica Dettin |
| author_facet | Leonardo Cassari Annj Zamuner Grazia M. L. Messina Martina Marsotto Hongyi Chen Giovanni Gonnella Trevor Coward Chiara Battocchio Jie Huang Giovanna Iucci Giovanni Marletta Lucy Di Silvio Monica Dettin |
| author_sort | Leonardo Cassari |
| collection | DOAJ |
| description | Polyetheretherketone (PEEK) is a thermoplastic polymer that has been recently employed for bone tissue engineering as a result of its biocompatibility and mechanical properties being comparable to human bone. PEEK, however, is a bio-inert material and, when implanted, does not interact with the host tissues, resulting in poor integration. In this work, the surfaces of 3D-printed PEEK disks were functionalized with: (i) an adhesive peptide reproducing [351–359] h-Vitronectin sequence (HVP) and (ii) HVP retro-inverted dimer (D2HVP), that combines the bioactivity of the native sequence (HVP) with the stability toward proteolytic degradation. Both sequences were designed to be anchored to the polymer surface through specific covalent bonds via oxime chemistry. All functionalized PEEK samples were characterized by Water Contact Angle (WCA) measurements, Atomic Force Microscopy (AFM), and X-ray Photoelectron Spectroscopy (XPS) to confirm the peptide enrichment. The biological results showed that both peptides were able to increase cell proliferation at 3 and 21 days. D2HVP functionalized PEEK resulted in an enhanced proliferation across all time points investigated with higher calcium deposition and more elongated cell morphology. |
| first_indexed | 2024-03-11T09:05:39Z |
| format | Article |
| id | doaj.art-1126b9bfea104172b56d4154e4a07860 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T09:05:39Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-1126b9bfea104172b56d4154e4a078602023-11-16T19:22:26ZengMDPI AGBiomolecules2218-273X2023-01-0113224610.3390/biom13020246Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive PeptidesLeonardo Cassari0Annj Zamuner1Grazia M. L. Messina2Martina Marsotto3Hongyi Chen4Giovanni Gonnella5Trevor Coward6Chiara Battocchio7Jie Huang8Giovanna Iucci9Giovanni Marletta10Lucy Di Silvio11Monica Dettin12Department of Industrial Engineering, University of Padova, Via Marzolo 9, 35131 Padova, ItalyDepartment of Civil, Environmental, and Architectural Engineering, University of Padova, Via Marzolo 9, 35131 Padova, ItalyLaboratory for Molecular Surface and Nanotechnology (LAMSUN), Department of Chemical Sciences, University of Catania and CSGI, Viale A. Doria, 6, 95125 Catania, ItalyDepartment of Science, Roma Tre University, Via della Vasca Navale 79, 00146 Roma, ItalyDepartment of Mechanical Engineering, University College London, London WC1E 6BT, UKFaculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKFaculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKDepartment of Science, Roma Tre University, Via della Vasca Navale 79, 00146 Roma, ItalyDepartment of Mechanical Engineering, University College London, London WC1E 6BT, UKDepartment of Science, Roma Tre University, Via della Vasca Navale 79, 00146 Roma, ItalyLaboratory for Molecular Surface and Nanotechnology (LAMSUN), Department of Chemical Sciences, University of Catania and CSGI, Viale A. Doria, 6, 95125 Catania, ItalyFaculty of Dentistry, Oral & Craniofacial Sciences, King’s College London, London SE1 9RT, UKDepartment of Industrial Engineering, University of Padova, Via Marzolo 9, 35131 Padova, ItalyPolyetheretherketone (PEEK) is a thermoplastic polymer that has been recently employed for bone tissue engineering as a result of its biocompatibility and mechanical properties being comparable to human bone. PEEK, however, is a bio-inert material and, when implanted, does not interact with the host tissues, resulting in poor integration. In this work, the surfaces of 3D-printed PEEK disks were functionalized with: (i) an adhesive peptide reproducing [351–359] h-Vitronectin sequence (HVP) and (ii) HVP retro-inverted dimer (D2HVP), that combines the bioactivity of the native sequence (HVP) with the stability toward proteolytic degradation. Both sequences were designed to be anchored to the polymer surface through specific covalent bonds via oxime chemistry. All functionalized PEEK samples were characterized by Water Contact Angle (WCA) measurements, Atomic Force Microscopy (AFM), and X-ray Photoelectron Spectroscopy (XPS) to confirm the peptide enrichment. The biological results showed that both peptides were able to increase cell proliferation at 3 and 21 days. D2HVP functionalized PEEK resulted in an enhanced proliferation across all time points investigated with higher calcium deposition and more elongated cell morphology.https://www.mdpi.com/2218-273X/13/2/246PEEKsurface functionalizationVitronectin peptideshuman osteoblasts3D-printingbone implant |
| spellingShingle | Leonardo Cassari Annj Zamuner Grazia M. L. Messina Martina Marsotto Hongyi Chen Giovanni Gonnella Trevor Coward Chiara Battocchio Jie Huang Giovanna Iucci Giovanni Marletta Lucy Di Silvio Monica Dettin Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides Biomolecules PEEK surface functionalization Vitronectin peptides human osteoblasts 3D-printing bone implant |
| title | Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides |
| title_full | Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides |
| title_fullStr | Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides |
| title_full_unstemmed | Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides |
| title_short | Bioactive PEEK: Surface Enrichment of Vitronectin-Derived Adhesive Peptides |
| title_sort | bioactive peek surface enrichment of vitronectin derived adhesive peptides |
| topic | PEEK surface functionalization Vitronectin peptides human osteoblasts 3D-printing bone implant |
| url | https://www.mdpi.com/2218-273X/13/2/246 |
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