Placental vascularization in middle and late gestation in the pig
The aims of this study were to determine the changes in the capillary area density in relation to fetal development, to determine immunoexpression of angiogenic factors and to compare their mRNA expression throughout pig gestation. Samples were collected from the maternal-chorioallantoic interface a...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2022-03-01
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| Series: | Reproduction and Fertility |
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| Online Access: | https://raf.bioscientifica.com/view/journals/raf/3/1/RAF-21-0092.xml |
| _version_ | 1830227901846388736 |
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| author | Mariana Rita Fiorimanti Andrea Lorena Cristofolini María José Moreira-Espinoza María Belén Rabaglino Claudio Gustavo Barbeito Cecilia Inés Merkis |
| author_facet | Mariana Rita Fiorimanti Andrea Lorena Cristofolini María José Moreira-Espinoza María Belén Rabaglino Claudio Gustavo Barbeito Cecilia Inés Merkis |
| author_sort | Mariana Rita Fiorimanti |
| collection | DOAJ |
| description | The aims of this study were to determine the changes in the capillary area density in relation to fetal development, to determine immunoexpression of angiogenic factors and to compare their mRNA expression throughout pig gestation. Samples were collected from the maternal-chorioallantoic interface at days 40, 77, 85 and 114 of pregnancy for immunohistochemistry analysis and the measurement of mRNA expression of VEGFA, ANGPT1, ANGPT2, FGF2 and its receptors KDR, TEK, FGFR1, FGFR2 respectively. Morphometric measurement of blood vessels was performed. We found a significant increase in capillary area density throughout gestation (P < 0.05). On the maternal side, at day 77, we observed a significant increase in the number of vessels from small vascular areas (P < 0.05) and the vascular area was significantly higher on day 85 (P < 0.05). On the fetal side, the number of vessels and the vascular area increased between days 40 and 77 (P < 0.05) and between days 77 and 114 (P < 0.05), respectively. Immunohistochemical findings revealed intense VEGFA staining and a trend for increased expression towards the end of gestation (P < 0.05). We also demonstrated a high VEGFA, FGF2, FGFR1, ANGPT1 and ANGPT2 mRNA expression at day 77 (P < 0.05). In conclusion, our findings suggest that an active angiogenesis would be present even until late-middle gestation at day 77 of pregnancy with the predominance of angiogenic stimulation by VEGFA/KDR, FGF2/FGFR1 and a balance between ANGPT1 and ANGPT2/TEK. |
| first_indexed | 2024-12-18T10:03:54Z |
| format | Article |
| id | doaj.art-11298b5aac73439595aa55e8c550277d |
| institution | Directory Open Access Journal |
| issn | 2633-8386 |
| language | English |
| last_indexed | 2024-12-18T10:03:54Z |
| publishDate | 2022-03-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-11298b5aac73439595aa55e8c550277d2022-12-21T21:11:35ZengBioscientificaReproduction and Fertility2633-83862022-03-0131https://doi.org/10.1530/RAF-21-0092Placental vascularization in middle and late gestation in the pigMariana Rita Fiorimanti0Andrea Lorena Cristofolini1María José Moreira-Espinoza2María Belén Rabaglino3Claudio Gustavo Barbeito4Cecilia Inés Merkis5Area of Electron Microscopy, Department of Animal Pathology, School of Agronomy and Veterinary, National University of Río Cuarto, Río Cuarto, Argentina; National Scientific and Technical Research Council (CONICET), ArgentinaArea of Electron Microscopy, Department of Animal Pathology, School of Agronomy and Veterinary, National University of Río Cuarto, Río Cuarto, Argentina; National Scientific and Technical Research Council (CONICET), ArgentinaInstitute of Cell Biology, Chair of Cell Biology, Histology and Embryology, School of Medical Science, National University of Córdoba, Ciudad Universitaria, Córdoba, ArgentinaNational Scientific and Technical Research Council (CONICET), ArgentinaNational Scientific and Technical Research Council (CONICET), Argentina; Laboratory of Descriptive, Comparative and Experimental Histology and Embriology, School of Veterinary Sciences, National University of La Plata, Buenos Aires, ArgentinaArea of Electron Microscopy, Department of Animal Pathology, School of Agronomy and Veterinary, National University of Río Cuarto, Río Cuarto, ArgentinaThe aims of this study were to determine the changes in the capillary area density in relation to fetal development, to determine immunoexpression of angiogenic factors and to compare their mRNA expression throughout pig gestation. Samples were collected from the maternal-chorioallantoic interface at days 40, 77, 85 and 114 of pregnancy for immunohistochemistry analysis and the measurement of mRNA expression of VEGFA, ANGPT1, ANGPT2, FGF2 and its receptors KDR, TEK, FGFR1, FGFR2 respectively. Morphometric measurement of blood vessels was performed. We found a significant increase in capillary area density throughout gestation (P < 0.05). On the maternal side, at day 77, we observed a significant increase in the number of vessels from small vascular areas (P < 0.05) and the vascular area was significantly higher on day 85 (P < 0.05). On the fetal side, the number of vessels and the vascular area increased between days 40 and 77 (P < 0.05) and between days 77 and 114 (P < 0.05), respectively. Immunohistochemical findings revealed intense VEGFA staining and a trend for increased expression towards the end of gestation (P < 0.05). We also demonstrated a high VEGFA, FGF2, FGFR1, ANGPT1 and ANGPT2 mRNA expression at day 77 (P < 0.05). In conclusion, our findings suggest that an active angiogenesis would be present even until late-middle gestation at day 77 of pregnancy with the predominance of angiogenic stimulation by VEGFA/KDR, FGF2/FGFR1 and a balance between ANGPT1 and ANGPT2/TEK.https://raf.bioscientifica.com/view/journals/raf/3/1/RAF-21-0092.xmlangiogenesisplacentapigvegfafgf2angpt1/2 |
| spellingShingle | Mariana Rita Fiorimanti Andrea Lorena Cristofolini María José Moreira-Espinoza María Belén Rabaglino Claudio Gustavo Barbeito Cecilia Inés Merkis Placental vascularization in middle and late gestation in the pig Reproduction and Fertility angiogenesis placenta pig vegfa fgf2 angpt1/2 |
| title | Placental vascularization in middle and late gestation in the pig |
| title_full | Placental vascularization in middle and late gestation in the pig |
| title_fullStr | Placental vascularization in middle and late gestation in the pig |
| title_full_unstemmed | Placental vascularization in middle and late gestation in the pig |
| title_short | Placental vascularization in middle and late gestation in the pig |
| title_sort | placental vascularization in middle and late gestation in the pig |
| topic | angiogenesis placenta pig vegfa fgf2 angpt1/2 |
| url | https://raf.bioscientifica.com/view/journals/raf/3/1/RAF-21-0092.xml |
| work_keys_str_mv | AT marianaritafiorimanti placentalvascularizationinmiddleandlategestationinthepig AT andrealorenacristofolini placentalvascularizationinmiddleandlategestationinthepig AT mariajosemoreiraespinoza placentalvascularizationinmiddleandlategestationinthepig AT mariabelenrabaglino placentalvascularizationinmiddleandlategestationinthepig AT claudiogustavobarbeito placentalvascularizationinmiddleandlategestationinthepig AT ceciliainesmerkis placentalvascularizationinmiddleandlategestationinthepig |