Generating Functional Multicellular Organoids from Human Placenta Villi
Abstract The interaction between trophoblasts, stroma cells, and immune cells at the maternal–fetal interface constitutes the functional units of the placenta, which is crucial for successful pregnancy outcomes. However, the investigation of this intricate interplay is restricted due to the absence...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-09-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202301565 |
| _version_ | 1797684924765962240 |
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| author | Lijun Huang Zhaowei Tu Liudan Wei Wei Sun Yifan Wang Shilei Bi Fang He Lili Du Jingsi Chen Julia Kzhyshkowska Haibin Wang Dunjin Chen Shuang Zhang |
| author_facet | Lijun Huang Zhaowei Tu Liudan Wei Wei Sun Yifan Wang Shilei Bi Fang He Lili Du Jingsi Chen Julia Kzhyshkowska Haibin Wang Dunjin Chen Shuang Zhang |
| author_sort | Lijun Huang |
| collection | DOAJ |
| description | Abstract The interaction between trophoblasts, stroma cells, and immune cells at the maternal–fetal interface constitutes the functional units of the placenta, which is crucial for successful pregnancy outcomes. However, the investigation of this intricate interplay is restricted due to the absence of efficient experimental models. To address this challenge, a robust, reliable methodology for generating placenta villi organoids (PVOs) from early, late, or diseased pregnancies using air–liquid surface culture is developed. PVOs contain cytotrophoblasts that can self‐renew and differentiate directly, along with stromal elements that retain native immune cells. Analysis of scRNA sequencing and WES data reveals that PVOs faithfully recapitulate the cellular components and genetic alterations of the corresponding source tissue. Additionally, PVOs derived from patients with preeclampsia exhibit specific pathological features such as inflammation, antiangiogenic imbalance, and decreased syncytin expression. The PVO‐based propagation of primary placenta villi should enable a deeper investigation of placenta development and exploration of the underlying pathogenesis and therapeutics of placenta‐originated diseases. |
| first_indexed | 2024-03-12T00:36:51Z |
| format | Article |
| id | doaj.art-112ae84dd4b247daa9a134e6d2d911e9 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-12T00:36:51Z |
| publishDate | 2023-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-112ae84dd4b247daa9a134e6d2d911e92023-09-15T09:28:59ZengWileyAdvanced Science2198-38442023-09-011026n/an/a10.1002/advs.202301565Generating Functional Multicellular Organoids from Human Placenta VilliLijun Huang0Zhaowei Tu1Liudan Wei2Wei Sun3Yifan Wang4Shilei Bi5Fang He6Lili Du7Jingsi Chen8Julia Kzhyshkowska9Haibin Wang10Dunjin Chen11Shuang Zhang12Department of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaInstitute of Transfusion Medicine and Immunology Medical Faculty Mannheim University of Heidelberg 68167 Mannheim GermanyFujian Provincial Key Laboratory of Reproductive Health Research Department of Obstetrics and Gynecology The First Affiliated Hospital of Xiamen University School of Medicine Xiamen University Xiamen 361005 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaDepartment of Obstetrics and Gynecology The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 ChinaAbstract The interaction between trophoblasts, stroma cells, and immune cells at the maternal–fetal interface constitutes the functional units of the placenta, which is crucial for successful pregnancy outcomes. However, the investigation of this intricate interplay is restricted due to the absence of efficient experimental models. To address this challenge, a robust, reliable methodology for generating placenta villi organoids (PVOs) from early, late, or diseased pregnancies using air–liquid surface culture is developed. PVOs contain cytotrophoblasts that can self‐renew and differentiate directly, along with stromal elements that retain native immune cells. Analysis of scRNA sequencing and WES data reveals that PVOs faithfully recapitulate the cellular components and genetic alterations of the corresponding source tissue. Additionally, PVOs derived from patients with preeclampsia exhibit specific pathological features such as inflammation, antiangiogenic imbalance, and decreased syncytin expression. The PVO‐based propagation of primary placenta villi should enable a deeper investigation of placenta development and exploration of the underlying pathogenesis and therapeutics of placenta‐originated diseases.https://doi.org/10.1002/advs.202301565placenta villiorganoidpreeclampsiatrophoblastimmune cell |
| spellingShingle | Lijun Huang Zhaowei Tu Liudan Wei Wei Sun Yifan Wang Shilei Bi Fang He Lili Du Jingsi Chen Julia Kzhyshkowska Haibin Wang Dunjin Chen Shuang Zhang Generating Functional Multicellular Organoids from Human Placenta Villi Advanced Science placenta villi organoid preeclampsia trophoblast immune cell |
| title | Generating Functional Multicellular Organoids from Human Placenta Villi |
| title_full | Generating Functional Multicellular Organoids from Human Placenta Villi |
| title_fullStr | Generating Functional Multicellular Organoids from Human Placenta Villi |
| title_full_unstemmed | Generating Functional Multicellular Organoids from Human Placenta Villi |
| title_short | Generating Functional Multicellular Organoids from Human Placenta Villi |
| title_sort | generating functional multicellular organoids from human placenta villi |
| topic | placenta villi organoid preeclampsia trophoblast immune cell |
| url | https://doi.org/10.1002/advs.202301565 |
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