Clonal bamboo production based on in vitro culture
Bamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bamb...
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Format: | Article |
Language: | English |
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Universidade Federal de Uberlândia
2020-03-01
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Series: | Bioscience Journal |
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Online Access: | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/48169 |
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author | Anatálya dos Santos Ribeiro Alexssandra Jéssica Rondon de Figueiredo Gabriela Cristina Rech Tormen André Luís Lopes da Silva Wellington Ferreira Campos Gilvano Ebling Brondani |
author_facet | Anatálya dos Santos Ribeiro Alexssandra Jéssica Rondon de Figueiredo Gabriela Cristina Rech Tormen André Luís Lopes da Silva Wellington Ferreira Campos Gilvano Ebling Brondani |
author_sort | Anatálya dos Santos Ribeiro |
collection | DOAJ |
description | Bamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bambusa vulgaris. The first experiment tested different systemic and contact fungicide solutions, based on exposure time, during the establishment phase. Established explants were subjected to evaluation of residual fungicide effect on subcultures during the multiplication and elongation phases. The second experiment evaluated the influence of activated carbon on ex vitro survival and on adventitious rooting. Explant immersion in liquid culture medium added with 1.0 mL of fungicide for 120 hours has favored the in vitro establishment and reduced fungal contamination. On the other hand, it favored the shoot emission of shoots per explant during the multiplication phase. Both rooting induction culture medium and mini-incubator system use were effective in enabling adventitious root formation. The presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate. |
first_indexed | 2024-12-19T15:10:08Z |
format | Article |
id | doaj.art-112f7a299af84c9ea2289d7ea5bf7137 |
institution | Directory Open Access Journal |
issn | 1981-3163 |
language | English |
last_indexed | 2024-12-19T15:10:08Z |
publishDate | 2020-03-01 |
publisher | Universidade Federal de Uberlândia |
record_format | Article |
series | Bioscience Journal |
spelling | doaj.art-112f7a299af84c9ea2289d7ea5bf71372022-12-21T20:16:20ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632020-03-0136410.14393/BJ-v36n4a2020-4816948169Clonal bamboo production based on in vitro cultureAnatálya dos Santos RibeiroAlexssandra Jéssica Rondon de FigueiredoGabriela Cristina Rech TormenAndré Luís Lopes da SilvaWellington Ferreira CamposGilvano Ebling BrondaniBamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bambusa vulgaris. The first experiment tested different systemic and contact fungicide solutions, based on exposure time, during the establishment phase. Established explants were subjected to evaluation of residual fungicide effect on subcultures during the multiplication and elongation phases. The second experiment evaluated the influence of activated carbon on ex vitro survival and on adventitious rooting. Explant immersion in liquid culture medium added with 1.0 mL of fungicide for 120 hours has favored the in vitro establishment and reduced fungal contamination. On the other hand, it favored the shoot emission of shoots per explant during the multiplication phase. Both rooting induction culture medium and mini-incubator system use were effective in enabling adventitious root formation. The presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/48169bambusa vulgariscloningin vitro establishmentmicropropagationmultiplicationadventitious rooting |
spellingShingle | Anatálya dos Santos Ribeiro Alexssandra Jéssica Rondon de Figueiredo Gabriela Cristina Rech Tormen André Luís Lopes da Silva Wellington Ferreira Campos Gilvano Ebling Brondani Clonal bamboo production based on in vitro culture Bioscience Journal bambusa vulgaris cloning in vitro establishment micropropagation multiplication adventitious rooting |
title | Clonal bamboo production based on in vitro culture |
title_full | Clonal bamboo production based on in vitro culture |
title_fullStr | Clonal bamboo production based on in vitro culture |
title_full_unstemmed | Clonal bamboo production based on in vitro culture |
title_short | Clonal bamboo production based on in vitro culture |
title_sort | clonal bamboo production based on in vitro culture |
topic | bambusa vulgaris cloning in vitro establishment micropropagation multiplication adventitious rooting |
url | http://www.seer.ufu.br/index.php/biosciencejournal/article/view/48169 |
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