Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS
Quantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and PUFA metabolites could be a valuable indicator of health status. However, a method for...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227523001657 |
| _version_ | 1797347099966177280 |
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| author | Kohta Nakatani Yoshihiro Izumi Hironobu Umakoshi Maki Yokomoto-Umakoshi Tomoko Nakaji Hiroki Kaneko Hiroshi Nakao Yoshihiro Ogawa Kazutaka Ikeda Takeshi Bamba |
| author_facet | Kohta Nakatani Yoshihiro Izumi Hironobu Umakoshi Maki Yokomoto-Umakoshi Tomoko Nakaji Hiroki Kaneko Hiroshi Nakao Yoshihiro Ogawa Kazutaka Ikeda Takeshi Bamba |
| author_sort | Kohta Nakatani |
| collection | DOAJ |
| description | Quantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and PUFA metabolites could be a valuable indicator of health status. However, a method for simultaneously measuring these bioactive lipids has not yet been developed. Here, we report a LC/MS/MS method that can simultaneously measure 144 bioactive lipids, including steroids, bile acids, and PUFA metabolites, from human plasma, and a sample preparation method for these targets. Protein removal by methanol precipitation and purification of bioactive lipids by solid-phase extraction improved the recovery of the targeted compounds in human plasma samples, demonstrating the importance of sample preparation methods for a wide range of bioactive lipid analyses. Using the developed method, we studied the plasma from healthy human volunteers and confirmed the presence of bioactive lipid molecules associated with sex differences and circadian rhythms. The developed method of bioactive lipid analysis can be applied to health monitoring and disease biomarker discovery in precision medicine. |
| first_indexed | 2024-03-08T11:42:03Z |
| format | Article |
| id | doaj.art-112f82abfea747e5bf537e56184becf3 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-03-08T11:42:03Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-112f82abfea747e5bf537e56184becf32024-01-25T05:22:33ZengElsevierJournal of Lipid Research0022-22752024-01-01651100492Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MSKohta Nakatani0Yoshihiro Izumi1Hironobu Umakoshi2Maki Yokomoto-Umakoshi3Tomoko Nakaji4Hiroki Kaneko5Hiroshi Nakao6Yoshihiro Ogawa7Kazutaka Ikeda8Takeshi Bamba9Division of Metabolomics/Mass Spectrometry Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, JapanDivision of Metabolomics/Mass Spectrometry Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan; For correspondence: Yoshihiro Izumi; Takeshi BambaDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDivision of Metabolomics/Mass Spectrometry Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, JapanDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanDepartment of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, JapanLaboratory of Biomolecule Analysis, Department of Applied Genomics, Kazusa DNA Research Institute, Kisarazu, Chiba, JapanDivision of Metabolomics/Mass Spectrometry Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan; For correspondence: Yoshihiro Izumi; Takeshi BambaQuantitative information on blood metabolites can be used in developing advanced medical strategies such as early detection and prevention of disease. Monitoring bioactive lipids such as steroids, bile acids, and PUFA metabolites could be a valuable indicator of health status. However, a method for simultaneously measuring these bioactive lipids has not yet been developed. Here, we report a LC/MS/MS method that can simultaneously measure 144 bioactive lipids, including steroids, bile acids, and PUFA metabolites, from human plasma, and a sample preparation method for these targets. Protein removal by methanol precipitation and purification of bioactive lipids by solid-phase extraction improved the recovery of the targeted compounds in human plasma samples, demonstrating the importance of sample preparation methods for a wide range of bioactive lipid analyses. Using the developed method, we studied the plasma from healthy human volunteers and confirmed the presence of bioactive lipid molecules associated with sex differences and circadian rhythms. The developed method of bioactive lipid analysis can be applied to health monitoring and disease biomarker discovery in precision medicine.http://www.sciencedirect.com/science/article/pii/S0022227523001657steroidbile acidpolyunsaturated fatty acidliquid chromatographytandem mass spectrometrysolid-phase extraction |
| spellingShingle | Kohta Nakatani Yoshihiro Izumi Hironobu Umakoshi Maki Yokomoto-Umakoshi Tomoko Nakaji Hiroki Kaneko Hiroshi Nakao Yoshihiro Ogawa Kazutaka Ikeda Takeshi Bamba Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS Journal of Lipid Research steroid bile acid polyunsaturated fatty acid liquid chromatography tandem mass spectrometry solid-phase extraction |
| title | Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS |
| title_full | Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS |
| title_fullStr | Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS |
| title_full_unstemmed | Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS |
| title_short | Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS |
| title_sort | wide scope targeted analysis of bioactive lipids in human plasma by lc ms ms |
| topic | steroid bile acid polyunsaturated fatty acid liquid chromatography tandem mass spectrometry solid-phase extraction |
| url | http://www.sciencedirect.com/science/article/pii/S0022227523001657 |
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