Genetic and phylogenetic analysis of D-Loop in Persian Leopard
Persian leopard (<em>Panthera pardus saxicolor</em>) is the largest <em>P. pardus</em> subspecies in the world. But according to the sharp decline and extinction of populations in some areas, more attention to this subspecies in aspect of genetic conservation is of utmost imp...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Shahid Bahonar University of Kerman
2012-01-01
|
| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_1168_64b71ab9ed53acd81753d6d69334fce1.pdf |
| _version_ | 1818276540224372736 |
|---|---|
| author | Mohammad Reza Nasiri Seyyed Amir Reza Parizadeh Morteza Mahdavi Hamid Ariyan Nezhad |
| author_facet | Mohammad Reza Nasiri Seyyed Amir Reza Parizadeh Morteza Mahdavi Hamid Ariyan Nezhad |
| author_sort | Mohammad Reza Nasiri |
| collection | DOAJ |
| description | Persian leopard (<em>Panthera pardus saxicolor</em>) is the largest <em>P. pardus</em> subspecies in the world. But according to the sharp decline and extinction of populations in some areas, more attention to this subspecies in aspect of genetic conservation is of utmost importance. The goal of this study was to genetic and phylogenetic analysis of D-Loop region in Persian Leopard. Six biological samples of leopard bone and skin were prepared from Tandooreh National Park in Khorasan Razavi province from 2008 to 2011. After DNA extraction, 800bp fragment of the mitochondrial D-Loop was amplified with universal primers, L15926 and H16498 under polymerase chain reaction. Amplified fragments were sequenced with ABI 3130 automated machine under Sanger method. After editing the sequences, the results were compared with sequences from other studies to determine the genetic distances. Variations were not observed in the nucleotide sequences obtained in this study. Using the Neighbor-Joining phylogenetic test results showed that the Persian leopard is in group of <em>P. pardus</em> and its haplotype is the same with <em>saxicolor</em>. |
| first_indexed | 2024-12-12T22:47:16Z |
| format | Article |
| id | doaj.art-1131300058dc4c5d8aaa925609f36c82 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-12T22:47:16Z |
| publishDate | 2012-01-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-1131300058dc4c5d8aaa925609f36c822022-12-22T00:09:09ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002012-01-0132819510.22103/jab.2012.11681168Genetic and phylogenetic analysis of D-Loop in Persian LeopardMohammad Reza NasiriSeyyed Amir Reza ParizadehMorteza MahdaviHamid Ariyan NezhadPersian leopard (<em>Panthera pardus saxicolor</em>) is the largest <em>P. pardus</em> subspecies in the world. But according to the sharp decline and extinction of populations in some areas, more attention to this subspecies in aspect of genetic conservation is of utmost importance. The goal of this study was to genetic and phylogenetic analysis of D-Loop region in Persian Leopard. Six biological samples of leopard bone and skin were prepared from Tandooreh National Park in Khorasan Razavi province from 2008 to 2011. After DNA extraction, 800bp fragment of the mitochondrial D-Loop was amplified with universal primers, L15926 and H16498 under polymerase chain reaction. Amplified fragments were sequenced with ABI 3130 automated machine under Sanger method. After editing the sequences, the results were compared with sequences from other studies to determine the genetic distances. Variations were not observed in the nucleotide sequences obtained in this study. Using the Neighbor-Joining phylogenetic test results showed that the Persian leopard is in group of <em>P. pardus</em> and its haplotype is the same with <em>saxicolor</em>.https://jab.uk.ac.ir/article_1168_64b71ab9ed53acd81753d6d69334fce1.pdfpersian leopardmitochondrial dnad-loopphylogenetic treepanthera pardus |
| spellingShingle | Mohammad Reza Nasiri Seyyed Amir Reza Parizadeh Morteza Mahdavi Hamid Ariyan Nezhad Genetic and phylogenetic analysis of D-Loop in Persian Leopard مجله بیوتکنولوژی کشاورزی persian leopard mitochondrial dna d-loop phylogenetic tree panthera pardus |
| title | Genetic and phylogenetic analysis of D-Loop in Persian Leopard |
| title_full | Genetic and phylogenetic analysis of D-Loop in Persian Leopard |
| title_fullStr | Genetic and phylogenetic analysis of D-Loop in Persian Leopard |
| title_full_unstemmed | Genetic and phylogenetic analysis of D-Loop in Persian Leopard |
| title_short | Genetic and phylogenetic analysis of D-Loop in Persian Leopard |
| title_sort | genetic and phylogenetic analysis of d loop in persian leopard |
| topic | persian leopard mitochondrial dna d-loop phylogenetic tree panthera pardus |
| url | https://jab.uk.ac.ir/article_1168_64b71ab9ed53acd81753d6d69334fce1.pdf |
| work_keys_str_mv | AT mohammadrezanasiri geneticandphylogeneticanalysisofdloopinpersianleopard AT seyyedamirrezaparizadeh geneticandphylogeneticanalysisofdloopinpersianleopard AT mortezamahdavi geneticandphylogeneticanalysisofdloopinpersianleopard AT hamidariyannezhad geneticandphylogeneticanalysisofdloopinpersianleopard |