Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury
The effect of transplantation dose on the spatiotemporal dynamics of human neural stem cell (hNSC) engraftment has not been quantitatively evaluated in the central nervous system. We investigated changes over time in engraftment/survival, proliferation, and migration of multipotent human central ner...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-09-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506115000926 |
| _version_ | 1828386829053722624 |
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| author | Katja M. Piltti Sabrina N. Avakian Gabriella M. Funes Antoinette Hu Nobuko Uchida Aileen J. Anderson Brian J. Cummings |
| author_facet | Katja M. Piltti Sabrina N. Avakian Gabriella M. Funes Antoinette Hu Nobuko Uchida Aileen J. Anderson Brian J. Cummings |
| author_sort | Katja M. Piltti |
| collection | DOAJ |
| description | The effect of transplantation dose on the spatiotemporal dynamics of human neural stem cell (hNSC) engraftment has not been quantitatively evaluated in the central nervous system. We investigated changes over time in engraftment/survival, proliferation, and migration of multipotent human central nervous system-derived neural stem cells (hCNS-SCns) transplanted at doses ranging from 10,000 to 500,000 cells in spinal cord injured immunodeficient mice. Transplant dose was inversely correlated with measures of donor cell proliferation at 2 weeks post-transplant (WPT) and dose-normalized engraftment at 16 WPT. Critically, mice receiving the highest cell dose exhibited an engraftment plateau, in which the total number of engrafted human cells never exceeded the initial dose. These data suggest that donor cell expansion was inversely regulated by target niche parameters and/or transplantation density. Investigation of the response of donor cells to the host microenvironment should be a key variable in defining target cell dose in pre-clinical models of CNS disease and injury. |
| first_indexed | 2024-12-10T05:44:05Z |
| format | Article |
| id | doaj.art-1136d545b49c40e9a699d4692a6dc2e2 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-10T05:44:05Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-1136d545b49c40e9a699d4692a6dc2e22022-12-22T02:00:12ZengElsevierStem Cell Research1873-50611876-77532015-09-0115234135310.1016/j.scr.2015.07.001Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injuryKatja M. Piltti0Sabrina N. Avakian1Gabriella M. Funes2Antoinette Hu3Nobuko Uchida4Aileen J. Anderson5Brian J. Cummings6Sue & Bill Gross Stem Cell Center, USASue & Bill Gross Stem Cell Center, USASue & Bill Gross Stem Cell Center, USASue & Bill Gross Stem Cell Center, USAStemCells Inc., 7707 Gateway, Newark, CA 94560, USASue & Bill Gross Stem Cell Center, USASue & Bill Gross Stem Cell Center, USAThe effect of transplantation dose on the spatiotemporal dynamics of human neural stem cell (hNSC) engraftment has not been quantitatively evaluated in the central nervous system. We investigated changes over time in engraftment/survival, proliferation, and migration of multipotent human central nervous system-derived neural stem cells (hCNS-SCns) transplanted at doses ranging from 10,000 to 500,000 cells in spinal cord injured immunodeficient mice. Transplant dose was inversely correlated with measures of donor cell proliferation at 2 weeks post-transplant (WPT) and dose-normalized engraftment at 16 WPT. Critically, mice receiving the highest cell dose exhibited an engraftment plateau, in which the total number of engrafted human cells never exceeded the initial dose. These data suggest that donor cell expansion was inversely regulated by target niche parameters and/or transplantation density. Investigation of the response of donor cells to the host microenvironment should be a key variable in defining target cell dose in pre-clinical models of CNS disease and injury.http://www.sciencedirect.com/science/article/pii/S1873506115000926hNSCSCIStem cell niche dynamicsStem cell transplantationCell doseSurvivalProliferationMigration |
| spellingShingle | Katja M. Piltti Sabrina N. Avakian Gabriella M. Funes Antoinette Hu Nobuko Uchida Aileen J. Anderson Brian J. Cummings Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury Stem Cell Research hNSC SCI Stem cell niche dynamics Stem cell transplantation Cell dose Survival Proliferation Migration |
| title | Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury |
| title_full | Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury |
| title_fullStr | Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury |
| title_full_unstemmed | Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury |
| title_short | Transplantation dose alters the dynamics of human neural stem cell engraftment, proliferation and migration after spinal cord injury |
| title_sort | transplantation dose alters the dynamics of human neural stem cell engraftment proliferation and migration after spinal cord injury |
| topic | hNSC SCI Stem cell niche dynamics Stem cell transplantation Cell dose Survival Proliferation Migration |
| url | http://www.sciencedirect.com/science/article/pii/S1873506115000926 |
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