Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil
Lavandin essential oil (LEO), a natural sterile hybrid obtained by crossbreeding <i>L. angustifolia × L. latifolia,</i> is mainly composed by active components belonging to the family of terpenes endowed with relevant anti-proliferative activity, which can be enhanced by proper applicati...
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MDPI AG
2020-10-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/13/11/352 |
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| author | Elisa Ovidi Valentina Laghezza Masci Anna Rita Taddei Patrizia Paolicelli Stefania Petralito Jordan Trilli Fabio Mastrogiovanni Antonio Tiezzi Maria Antonietta Casadei Pierluigi Giacomello Stefania Garzoli |
| author_facet | Elisa Ovidi Valentina Laghezza Masci Anna Rita Taddei Patrizia Paolicelli Stefania Petralito Jordan Trilli Fabio Mastrogiovanni Antonio Tiezzi Maria Antonietta Casadei Pierluigi Giacomello Stefania Garzoli |
| author_sort | Elisa Ovidi |
| collection | DOAJ |
| description | Lavandin essential oil (LEO), a natural sterile hybrid obtained by crossbreeding <i>L. angustifolia × L. latifolia,</i> is mainly composed by active components belonging to the family of terpenes endowed with relevant anti-proliferative activity, which can be enhanced by proper application of nanotechnology. In particular, this study reports the chemical characterization and the screening of the anti-proliferative activity on different human cell lines of pure and nano-formulated lavandin essential oil (EO). LEO and its formulation (NanoLEO) were analyzed by HS/GC-MS (Headspace/Gas Chromatography-Mass Spectrometry) to describe and compare their chemical volatile composition. The most abundant compounds were linalool and 1,8-cineole (LEO: 28.6%; 27.4%) (NanoLEO: 60.4%; 12.6%) followed by α-pinene (LEO: 9.6%; NanoLEO: 4.5%), camphor (LEO: 6.5%; NanoLEO: 7.0%) and linalyl acetate (LEO: 6.5%; NanoLEO: 3.6%). The cytotoxic effects of LEO and NanoLEO were investigated on human neuroblastoma cells (SHSY5Y), human breast adenocarcinoma cells (MCF-7), human lymphoblastic leukemia cells (CCRF CEM), human colorectal adenocarcinoma cells (Caco-2) and one normal breast epithelial cell (MCF10A) by the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)-assay. Caco-2, MCF7 and MCF10A normal cells resulted more resistant to the treatment with LEO, while CCRF-CEM and SHSY5Y cells were more sensitive. The antiproliferative effect of LEO resulted amplified when the essential oil was supplied as nanoformulation, mainly in Caco-2 cells. Scanning and transmission electron microscopy investigations were carried out on Caco-2 cells to outline at ultrastructural level possible affections induced by LEO and NanoLEO treatments. |
| first_indexed | 2024-03-10T15:14:32Z |
| format | Article |
| id | doaj.art-113b80cc78d846aa816b12126fee12f9 |
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| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T15:14:32Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj.art-113b80cc78d846aa816b12126fee12f92023-11-20T19:01:00ZengMDPI AGPharmaceuticals1424-82472020-10-01131135210.3390/ph13110352Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential OilElisa Ovidi0Valentina Laghezza Masci1Anna Rita Taddei2Patrizia Paolicelli3Stefania Petralito4Jordan Trilli5Fabio Mastrogiovanni6Antonio Tiezzi7Maria Antonietta Casadei8Pierluigi Giacomello9Stefania Garzoli10Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, ItalyHigh Equipment Centre, Tuscia University, 01100 Viterbo, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, ItalyDepartment for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, ItalyDepartment for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, ItalyDepartment of Drug Chemistry and Technology, Sapienza University, 00185 Roma RM, ItalyLavandin essential oil (LEO), a natural sterile hybrid obtained by crossbreeding <i>L. angustifolia × L. latifolia,</i> is mainly composed by active components belonging to the family of terpenes endowed with relevant anti-proliferative activity, which can be enhanced by proper application of nanotechnology. In particular, this study reports the chemical characterization and the screening of the anti-proliferative activity on different human cell lines of pure and nano-formulated lavandin essential oil (EO). LEO and its formulation (NanoLEO) were analyzed by HS/GC-MS (Headspace/Gas Chromatography-Mass Spectrometry) to describe and compare their chemical volatile composition. The most abundant compounds were linalool and 1,8-cineole (LEO: 28.6%; 27.4%) (NanoLEO: 60.4%; 12.6%) followed by α-pinene (LEO: 9.6%; NanoLEO: 4.5%), camphor (LEO: 6.5%; NanoLEO: 7.0%) and linalyl acetate (LEO: 6.5%; NanoLEO: 3.6%). The cytotoxic effects of LEO and NanoLEO were investigated on human neuroblastoma cells (SHSY5Y), human breast adenocarcinoma cells (MCF-7), human lymphoblastic leukemia cells (CCRF CEM), human colorectal adenocarcinoma cells (Caco-2) and one normal breast epithelial cell (MCF10A) by the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)-assay. Caco-2, MCF7 and MCF10A normal cells resulted more resistant to the treatment with LEO, while CCRF-CEM and SHSY5Y cells were more sensitive. The antiproliferative effect of LEO resulted amplified when the essential oil was supplied as nanoformulation, mainly in Caco-2 cells. Scanning and transmission electron microscopy investigations were carried out on Caco-2 cells to outline at ultrastructural level possible affections induced by LEO and NanoLEO treatments.https://www.mdpi.com/1424-8247/13/11/352lavandin essential oilantiproliferative activityHS-GC/MS analysisnanoemulsion |
| spellingShingle | Elisa Ovidi Valentina Laghezza Masci Anna Rita Taddei Patrizia Paolicelli Stefania Petralito Jordan Trilli Fabio Mastrogiovanni Antonio Tiezzi Maria Antonietta Casadei Pierluigi Giacomello Stefania Garzoli Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil Pharmaceuticals lavandin essential oil antiproliferative activity HS-GC/MS analysis nanoemulsion |
| title | Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil |
| title_full | Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil |
| title_fullStr | Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil |
| title_full_unstemmed | Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil |
| title_short | Chemical Investigation and Screening of Anti-Proliferative Activity on Human Cell Lines of Pure and Nano-Formulated Lavandin Essential Oil |
| title_sort | chemical investigation and screening of anti proliferative activity on human cell lines of pure and nano formulated lavandin essential oil |
| topic | lavandin essential oil antiproliferative activity HS-GC/MS analysis nanoemulsion |
| url | https://www.mdpi.com/1424-8247/13/11/352 |
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