COMPARISON OF TWO TOTAL RNA EXTRACTION PROTOCOLS FROM CHO-K1 CELLS FOR RT-PCR: CUT-OFF COST FOR RESEARCHERS

ABSTRACT: Various methods have been described to extract RNA from adherent mammalian cells. RNA isolation in conjunction with reverse transcription polymerase chain reaction (RT-PCR) is a valuable tool used to study gene expression profiling. This approach is now being used in mammalian cell bioproc...

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Bibliographic Details
Main Authors: Vasila Packeer Mohamed, Yumi Z. H-Y. Hashim, A. Amid, M. Mel
Format: Article
Language:English
Published: IIUM Press, International Islamic University Malaysia 2014-05-01
Series:International Islamic University Malaysia Engineering Journal
Online Access:http://journals.iium.edu.my/ejournal/index.php/iiumej/article/view/452