Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
Objectives: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell r...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023039993 |
| _version_ | 1797802762559291392 |
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| author | Qianyu Ye Jiali Wang Meijing Chen Weijian Nie Huanxi Zhang Xiaojun Su Liuting Ling Xiangjun Liu Longshan Liu Changxi Wang Yifang Gao |
| author_facet | Qianyu Ye Jiali Wang Meijing Chen Weijian Nie Huanxi Zhang Xiaojun Su Liuting Ling Xiangjun Liu Longshan Liu Changxi Wang Yifang Gao |
| author_sort | Qianyu Ye |
| collection | DOAJ |
| description | Objectives: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. Methods: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. Results: The levels of IFN-γ, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-γ secretion than did ELISpot. Conclusions: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications. |
| first_indexed | 2024-03-13T05:11:40Z |
| format | Article |
| id | doaj.art-113cbcb8e7f34a2b902f33e89eeed041 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T05:11:40Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-113cbcb8e7f34a2b902f33e89eeed0412023-06-16T05:10:18ZengElsevierHeliyon2405-84402023-06-0196e16792Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirusQianyu Ye0Jiali Wang1Meijing Chen2Weijian Nie3Huanxi Zhang4Xiaojun Su5Liuting Ling6Xiangjun Liu7Longshan Liu8Changxi Wang9Yifang Gao10Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Nephrology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaGuangzhou BFR Gene Dignostics, Guangzhou, ChinaOrgan Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaOrgan Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaOrgan Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaOrgan Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaBeijing BFR Gene Diagnostics, Beijing, ChinaOrgan Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Corresponding author. Organ Transplantation Centre, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhong Shan 2nd Street, Guangzhou, 510080, China.Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Corresponding author. Organ Transplantation Centre, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhong Shan 2nd Street, Guangzhou, 510080, China.Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Corresponding author. Organ Transplantation Centre, Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, No. 58, Zhong Shan 2nd Street, Guangzhou, 510080, China.Objectives: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell response in healthy individuals. The FlowSpot results were compared with those of serological analysis and enzyme-linked immunospot (ELISpot) assay. Methods: Experimental results and parameter analysis were investigated by using serological, ELISpot, and FlowSpot assays. Results: The levels of IFN-γ, which is released from CMV-specific T-cells, were measured, and the results and parameter analysis showed a good correlation between FlowSpot and ELISpot. However, FlowSpot was more sensitive and better reflected the strength of IFN-γ secretion than did ELISpot. Conclusions: Compared to ELISpot, FlowSpot has a high sensitivity and is cost and time effective. Thus, this method can be used in wider clinical and scientific applications.http://www.sciencedirect.com/science/article/pii/S2405844023039993FlowSpotELISpotSerologic analysisCMV-Specific T-cellsIFN-γ |
| spellingShingle | Qianyu Ye Jiali Wang Meijing Chen Weijian Nie Huanxi Zhang Xiaojun Su Liuting Ling Xiangjun Liu Longshan Liu Changxi Wang Yifang Gao Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus Heliyon FlowSpot ELISpot Serologic analysis CMV-Specific T-cells IFN-γ |
| title | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
| title_full | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
| title_fullStr | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
| title_full_unstemmed | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
| title_short | Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus |
| title_sort | interferon gamma flowspot assay for the measurement of the t cell response to cytomegalovirus |
| topic | FlowSpot ELISpot Serologic analysis CMV-Specific T-cells IFN-γ |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023039993 |
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