Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus

Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus

Objectives: We describe a new method, FlowSpot, to assess CMV-specific T-cell response by quantification of interferon-gamma (IFN-γ). CMV-specific, T-cell-released IFN-γ was captured by flow beads and measured via flow cytometry. In the present study, we used FlowSpot to assess CMV-specific T-cell r...

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Bibliographic Details
Main Authors: Qianyu Ye, Jiali Wang, Meijing Chen, Weijian Nie, Huanxi Zhang, Xiaojun Su, Liuting Ling, Xiangjun Liu, Longshan Liu, Changxi Wang, Yifang Gao
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:Heliyon
Subjects:
FlowSpot
ELISpot
Serologic analysis
CMV-Specific T-cells
IFN-γ
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844023039993

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