Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver
Background/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the li...
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Format: | Article |
Language: | English |
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Wolters Kluwer Medknow Publications
2012-01-01
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Series: | The Saudi Journal of Gastroenterology |
Subjects: | |
Online Access: | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2012;volume=18;issue=4;spage=263;epage=267;aulast=Ali |
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author | Gibran Ali Muhammad S Masoud |
author_facet | Gibran Ali Muhammad S Masoud |
author_sort | Gibran Ali |
collection | DOAJ |
description | Background/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the liver, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks). After sacrificing the animals, quantitative data of percentage fibrosis area and the number of cells expressing albumin was obtained. One-way analysis of variance was applied to calculate the significance of the data. Results: GFP expressing cells clearly indicated migrated BMSCs with strong expression of albumin after 28 days post-transplantation shown by anti-albumin antibody. Double fluorescent immunohistochemistry showed reduced expression of αSMA on GFP-positive cells. Four weeks after BMSC transplantation, mice had significantly reduced liver fibrosis as compared with that of mice treated with CCl 4 assessed by Sirius red staining. Conclusion: Mice with BMSC transplantation with continuous CCl 4 injection had reduced liver fibrosis and a significantly improved expression of albumin compared with mice treated with CCl 4 alone. These findings strengthen the concept of cellular therapy in liver fibrosis. |
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format | Article |
id | doaj.art-1142a99eac0d49b5ae6228ebffac334b |
institution | Directory Open Access Journal |
issn | 1319-3767 1998-4049 |
language | English |
last_indexed | 2024-04-13T02:27:17Z |
publishDate | 2012-01-01 |
publisher | Wolters Kluwer Medknow Publications |
record_format | Article |
series | The Saudi Journal of Gastroenterology |
spelling | doaj.art-1142a99eac0d49b5ae6228ebffac334b2022-12-22T03:06:44ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492012-01-0118426326710.4103/1319-3767.98433Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liverGibran AliMuhammad S MasoudBackground/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the liver, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks). After sacrificing the animals, quantitative data of percentage fibrosis area and the number of cells expressing albumin was obtained. One-way analysis of variance was applied to calculate the significance of the data. Results: GFP expressing cells clearly indicated migrated BMSCs with strong expression of albumin after 28 days post-transplantation shown by anti-albumin antibody. Double fluorescent immunohistochemistry showed reduced expression of αSMA on GFP-positive cells. Four weeks after BMSC transplantation, mice had significantly reduced liver fibrosis as compared with that of mice treated with CCl 4 assessed by Sirius red staining. Conclusion: Mice with BMSC transplantation with continuous CCl 4 injection had reduced liver fibrosis and a significantly improved expression of albumin compared with mice treated with CCl 4 alone. These findings strengthen the concept of cellular therapy in liver fibrosis.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2012;volume=18;issue=4;spage=263;epage=267;aulast=AliAlbumingreen fluorescent protein (GFP)liver fibrosismiceαSmooth Muscle Actin (α SMA) |
spellingShingle | Gibran Ali Muhammad S Masoud Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver The Saudi Journal of Gastroenterology Albumin green fluorescent protein (GFP) liver fibrosis mice αSmooth Muscle Actin (α SMA) |
title | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
title_full | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
title_fullStr | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
title_full_unstemmed | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
title_short | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
title_sort | bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in ccl 4 induced fibrotic liver |
topic | Albumin green fluorescent protein (GFP) liver fibrosis mice αSmooth Muscle Actin (α SMA) |
url | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2012;volume=18;issue=4;spage=263;epage=267;aulast=Ali |
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