Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver
Background/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the li...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2012-01-01
|
| Series: | The Saudi Journal of Gastroenterology |
| Subjects: | |
| Online Access: | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2012;volume=18;issue=4;spage=263;epage=267;aulast=Ali |
| _version_ | 1811284281558827008 |
|---|---|
| author | Gibran Ali Muhammad S Masoud |
| author_facet | Gibran Ali Muhammad S Masoud |
| author_sort | Gibran Ali |
| collection | DOAJ |
| description | Background/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the liver, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks). After sacrificing the animals, quantitative data of percentage fibrosis area and the number of cells expressing albumin was obtained. One-way analysis of variance was applied to calculate the significance of the data. Results: GFP expressing cells clearly indicated migrated BMSCs with strong expression of albumin after 28 days post-transplantation shown by anti-albumin antibody. Double fluorescent immunohistochemistry showed reduced expression of αSMA on GFP-positive cells. Four weeks after BMSC transplantation, mice had significantly reduced liver fibrosis as compared with that of mice treated with CCl 4 assessed by Sirius red staining. Conclusion: Mice with BMSC transplantation with continuous CCl 4 injection had reduced liver fibrosis and a significantly improved expression of albumin compared with mice treated with CCl 4 alone. These findings strengthen the concept of cellular therapy in liver fibrosis. |
| first_indexed | 2024-04-13T02:27:17Z |
| format | Article |
| id | doaj.art-1142a99eac0d49b5ae6228ebffac334b |
| institution | Directory Open Access Journal |
| issn | 1319-3767 1998-4049 |
| language | English |
| last_indexed | 2024-04-13T02:27:17Z |
| publishDate | 2012-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | The Saudi Journal of Gastroenterology |
| spelling | doaj.art-1142a99eac0d49b5ae6228ebffac334b2022-12-22T03:06:44ZengWolters Kluwer Medknow PublicationsThe Saudi Journal of Gastroenterology1319-37671998-40492012-01-0118426326710.4103/1319-3767.98433Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liverGibran AliMuhammad S MasoudBackground/Aim: We investigated the effect of bone marrow-derived stem cell (BMSC) transplantation on carbon tetrachloride (CCl 4 )-induced liver fibrosis. Patients and Methods: BMSCs of green fluorescent protein (GFP) mice were transplanted into 4-week CCl 4 -treated C57BL/6 mice directly to the liver, and the mice were treated for 4 more weeks with CCl 4 (total, 8 weeks). After sacrificing the animals, quantitative data of percentage fibrosis area and the number of cells expressing albumin was obtained. One-way analysis of variance was applied to calculate the significance of the data. Results: GFP expressing cells clearly indicated migrated BMSCs with strong expression of albumin after 28 days post-transplantation shown by anti-albumin antibody. Double fluorescent immunohistochemistry showed reduced expression of αSMA on GFP-positive cells. Four weeks after BMSC transplantation, mice had significantly reduced liver fibrosis as compared with that of mice treated with CCl 4 assessed by Sirius red staining. Conclusion: Mice with BMSC transplantation with continuous CCl 4 injection had reduced liver fibrosis and a significantly improved expression of albumin compared with mice treated with CCl 4 alone. These findings strengthen the concept of cellular therapy in liver fibrosis.http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2012;volume=18;issue=4;spage=263;epage=267;aulast=AliAlbumingreen fluorescent protein (GFP)liver fibrosismiceαSmooth Muscle Actin (α SMA) |
| spellingShingle | Gibran Ali Muhammad S Masoud Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver The Saudi Journal of Gastroenterology Albumin green fluorescent protein (GFP) liver fibrosis mice αSmooth Muscle Actin (α SMA) |
| title | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
| title_full | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
| title_fullStr | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
| title_full_unstemmed | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
| title_short | Bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in CCl 4 -induced fibrotic liver |
| title_sort | bone marrow cells ameliorate liver fibrosis and express albumin after transplantation in ccl 4 induced fibrotic liver |
| topic | Albumin green fluorescent protein (GFP) liver fibrosis mice αSmooth Muscle Actin (α SMA) |
| url | http://www.saudijgastro.com/article.asp?issn=1319-3767;year=2012;volume=18;issue=4;spage=263;epage=267;aulast=Ali |
| work_keys_str_mv | AT gibranali bonemarrowcellsameliorateliverfibrosisandexpressalbuminaftertransplantationinccl4inducedfibroticliver AT muhammadsmasoud bonemarrowcellsameliorateliverfibrosisandexpressalbuminaftertransplantationinccl4inducedfibroticliver |