In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil
Micropropagation studies were carried out using the seeds for establishing an in vitro culture of Vriesea gigantea and Vriesea philippocoburgii. Germination rate of V. gigantea was higher than of V. philippocoburgii. Plantlets of V. philippocoburgii gave rise to many adventitious shoots when cultiva...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2005-09-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600006 |
| _version_ | 1811217618700337152 |
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| author | Annette Droste Anelise Machado da Silva Adriana Vieira Matos Júlia Winck de Almeida |
| author_facet | Annette Droste Anelise Machado da Silva Adriana Vieira Matos Júlia Winck de Almeida |
| author_sort | Annette Droste |
| collection | DOAJ |
| description | Micropropagation studies were carried out using the seeds for establishing an in vitro culture of Vriesea gigantea and Vriesea philippocoburgii. Germination rate of V. gigantea was higher than of V. philippocoburgii. Plantlets of V. philippocoburgii gave rise to many adventitious shoots when cultivated in Knudson basal medium. In contrast, for V. gigantea, a higher salts-concentration was needed, so that the number of shoots was increased by Murashige and Skoog medium. Addition of activated charcoal and naphthaleneacetic acid in regeneration medium allowed the growth of shoots and the formation of roots, confirming the success of in vitro culture. The differences in expression of the genotypes reinforce the need of more research in order to set up the conditions that could offer the best response of the specific tissues.<br>A importância das bromélias como plantas ornamentais e o fato de várias espécies nativas do Rio Grande do Sul estarem ameaçadas de extinção leva ao interesse pela sua micropropagação. Uma cultura in vitro de Vriesea gigantea e Vriesea philippocoburgii foi estabelecida a partir de sementes. A taxa de germinação de V. gigantea foi superior à taxa de germinação de V. philippocoburgii. Plântulas de V. philippocoburgii deram origem a vários brotos adventíceos quando cultivadas em meio basal de Knudson. Por outro lado, para V. gigantea, foi necessária uma maior concentração de sais, sendo que o número de brotos foi aumentado com o meio Murashige and Skoog. A adição de carvão ativado e ácido naftalenoacético ao meio de regeneração permitiu o desenvolvimento de brotos e a formação raízes, confirmando o sucesso da cultura in vitro. A expressão diferencial dos genótipos justifica a necessidade de continuadas pesquisas para que se estabeleçam as condições ideais para os tecidos específicos. |
| first_indexed | 2024-04-12T06:57:47Z |
| format | Article |
| id | doaj.art-11434f154e7b4760916432c1c302a3fb |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-04-12T06:57:47Z |
| publishDate | 2005-09-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-11434f154e7b4760916432c1c302a3fb2022-12-22T03:43:05ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242005-09-0148571772210.1590/S1516-89132005000600006In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern BrazilAnnette DrosteAnelise Machado da SilvaAdriana Vieira MatosJúlia Winck de AlmeidaMicropropagation studies were carried out using the seeds for establishing an in vitro culture of Vriesea gigantea and Vriesea philippocoburgii. Germination rate of V. gigantea was higher than of V. philippocoburgii. Plantlets of V. philippocoburgii gave rise to many adventitious shoots when cultivated in Knudson basal medium. In contrast, for V. gigantea, a higher salts-concentration was needed, so that the number of shoots was increased by Murashige and Skoog medium. Addition of activated charcoal and naphthaleneacetic acid in regeneration medium allowed the growth of shoots and the formation of roots, confirming the success of in vitro culture. The differences in expression of the genotypes reinforce the need of more research in order to set up the conditions that could offer the best response of the specific tissues.<br>A importância das bromélias como plantas ornamentais e o fato de várias espécies nativas do Rio Grande do Sul estarem ameaçadas de extinção leva ao interesse pela sua micropropagação. Uma cultura in vitro de Vriesea gigantea e Vriesea philippocoburgii foi estabelecida a partir de sementes. A taxa de germinação de V. gigantea foi superior à taxa de germinação de V. philippocoburgii. Plântulas de V. philippocoburgii deram origem a vários brotos adventíceos quando cultivadas em meio basal de Knudson. Por outro lado, para V. gigantea, foi necessária uma maior concentração de sais, sendo que o número de brotos foi aumentado com o meio Murashige and Skoog. A adição de carvão ativado e ácido naftalenoacético ao meio de regeneração permitiu o desenvolvimento de brotos e a formação raízes, confirmando o sucesso da cultura in vitro. A expressão diferencial dos genótipos justifica a necessidade de continuadas pesquisas para que se estabeleçam as condições ideais para os tecidos específicos.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600006In vitro cultureconservationpropagationBromeliaceaeVriesea |
| spellingShingle | Annette Droste Anelise Machado da Silva Adriana Vieira Matos Júlia Winck de Almeida In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil Brazilian Archives of Biology and Technology In vitro culture conservation propagation Bromeliaceae Vriesea |
| title | In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil |
| title_full | In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil |
| title_fullStr | In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil |
| title_full_unstemmed | In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil |
| title_short | In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil |
| title_sort | in vitro culture of vriesea gigantea and vriesea philippocoburgii two vulnerable bromeliads native to southern brazil |
| topic | In vitro culture conservation propagation Bromeliaceae Vriesea |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600006 |
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