A gastrointestinal rotavirus infection mouse model for immune modulation studies
<p>Abstract</p> <p>Background</p> <p>Rotaviruses are the single most important cause of severe diarrhea in young children worldwide. The current study was conducted to assess whether colostrum containing rotavirus-specific antibodies (Gastrogard-R<sup>®</sup>...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-03-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/8/1/109 |
| _version_ | 1828358995577929728 |
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| author | van Amerongen Geert Crienen Annelies McNeal Monica M Knipping Karen Garssen Johan van't Land Belinda |
| author_facet | van Amerongen Geert Crienen Annelies McNeal Monica M Knipping Karen Garssen Johan van't Land Belinda |
| author_sort | van Amerongen Geert |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Rotaviruses are the single most important cause of severe diarrhea in young children worldwide. The current study was conducted to assess whether colostrum containing rotavirus-specific antibodies (Gastrogard-R<sup>®</sup>) could protect against rotavirus infection. In addition, this illness model was used to study modulatory effects of intervention on several immune parameters after re-infection.</p> <p>Methods</p> <p>BALB/c mice were treated by gavage once daily with Gastrogard-R<sup>® </sup>from the age of 4 to 10 days, and were inoculated with rhesus rotavirus (RRV) at 7 days of age. A secondary inoculation with epizootic-diarrhea infant-mouse (EDIM) virus was administered at 17 days of age. Disease symptoms were scored daily and viral shedding was measured in fecal samples during the post-inoculation periods. Rotavirus-specific IgM, IgG and IgG subclasses in serum, T cell proliferation and rotavirus-specific delayed-type hypersensitivity (DTH) responses were also measured.</p> <p>Results</p> <p>Primary inoculation with RRV induced a mild but consistent level of diarrhea during 3-4 days post-inoculation. All mice receiving Gastrogard-R<sup>® </sup>were 100% protected against rotavirus-induced diarrhea. Mice receiving both RRV and EDIM inoculation had a lower faecal-viral load following EDIM inoculation then mice receiving EDIM alone or Gastrogard-R<sup>®</sup>. Mice receiving Gastrogard-R<sup>® </sup>however displayed an enhanced rotavirus-specific T-cell proliferation whereas rotavirus-specific antibody subtypes were not affected.</p> <p>Conclusions</p> <p>Preventing RRV-induced diarrhea by Gastrogard-R<sup>® </sup>early in life showed a diminished protection against EDIM re-infection, but a rotavirus-specific immune response was developed including both B cell and T cell responses. In general, this intervention model can be used for studying clinical symptoms as well as the immune responses required for protection against viral re-infection.</p> |
| first_indexed | 2024-04-14T03:35:19Z |
| format | Article |
| id | doaj.art-1145cdd58bda4785bb28f4c98b3231dd |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-14T03:35:19Z |
| publishDate | 2011-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-1145cdd58bda4785bb28f4c98b3231dd2022-12-22T02:14:45ZengBMCVirology Journal1743-422X2011-03-018110910.1186/1743-422X-8-109A gastrointestinal rotavirus infection mouse model for immune modulation studiesvan Amerongen GeertCrienen AnneliesMcNeal Monica MKnipping KarenGarssen Johanvan't Land Belinda<p>Abstract</p> <p>Background</p> <p>Rotaviruses are the single most important cause of severe diarrhea in young children worldwide. The current study was conducted to assess whether colostrum containing rotavirus-specific antibodies (Gastrogard-R<sup>®</sup>) could protect against rotavirus infection. In addition, this illness model was used to study modulatory effects of intervention on several immune parameters after re-infection.</p> <p>Methods</p> <p>BALB/c mice were treated by gavage once daily with Gastrogard-R<sup>® </sup>from the age of 4 to 10 days, and were inoculated with rhesus rotavirus (RRV) at 7 days of age. A secondary inoculation with epizootic-diarrhea infant-mouse (EDIM) virus was administered at 17 days of age. Disease symptoms were scored daily and viral shedding was measured in fecal samples during the post-inoculation periods. Rotavirus-specific IgM, IgG and IgG subclasses in serum, T cell proliferation and rotavirus-specific delayed-type hypersensitivity (DTH) responses were also measured.</p> <p>Results</p> <p>Primary inoculation with RRV induced a mild but consistent level of diarrhea during 3-4 days post-inoculation. All mice receiving Gastrogard-R<sup>® </sup>were 100% protected against rotavirus-induced diarrhea. Mice receiving both RRV and EDIM inoculation had a lower faecal-viral load following EDIM inoculation then mice receiving EDIM alone or Gastrogard-R<sup>®</sup>. Mice receiving Gastrogard-R<sup>® </sup>however displayed an enhanced rotavirus-specific T-cell proliferation whereas rotavirus-specific antibody subtypes were not affected.</p> <p>Conclusions</p> <p>Preventing RRV-induced diarrhea by Gastrogard-R<sup>® </sup>early in life showed a diminished protection against EDIM re-infection, but a rotavirus-specific immune response was developed including both B cell and T cell responses. In general, this intervention model can be used for studying clinical symptoms as well as the immune responses required for protection against viral re-infection.</p>http://www.virologyj.com/content/8/1/109 |
| spellingShingle | van Amerongen Geert Crienen Annelies McNeal Monica M Knipping Karen Garssen Johan van't Land Belinda A gastrointestinal rotavirus infection mouse model for immune modulation studies Virology Journal |
| title | A gastrointestinal rotavirus infection mouse model for immune modulation studies |
| title_full | A gastrointestinal rotavirus infection mouse model for immune modulation studies |
| title_fullStr | A gastrointestinal rotavirus infection mouse model for immune modulation studies |
| title_full_unstemmed | A gastrointestinal rotavirus infection mouse model for immune modulation studies |
| title_short | A gastrointestinal rotavirus infection mouse model for immune modulation studies |
| title_sort | gastrointestinal rotavirus infection mouse model for immune modulation studies |
| url | http://www.virologyj.com/content/8/1/109 |
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