A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid
OBJECTIVE: To evaluate the performance of a newly developed point-of-care test (POCT) for the detection of measles-specific IgM antibodies in serum and oral fluid specimens and to assess if measles virus nucleic acid could be recovered from used POCT strips. METHODS: The POCT was used to test 170 se...
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| Format: | Article |
| Language: | English |
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The World Health Organization
2011-09-01
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| Series: | Bulletin of the World Health Organization |
| Online Access: | http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862011000900014&lng=en&tlng=en |
| _version_ | 1797281792324009984 |
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| author | Lenesha Warrener Rimantas Slibinskas Kaw Bing Chua Wondatir Nigatu Kevin E Brown Kestutis Sasnauskas Dhanraj Samuel David Brown |
| author_facet | Lenesha Warrener Rimantas Slibinskas Kaw Bing Chua Wondatir Nigatu Kevin E Brown Kestutis Sasnauskas Dhanraj Samuel David Brown |
| author_sort | Lenesha Warrener |
| collection | DOAJ |
| description | OBJECTIVE: To evaluate the performance of a newly developed point-of-care test (POCT) for the detection of measles-specific IgM antibodies in serum and oral fluid specimens and to assess if measles virus nucleic acid could be recovered from used POCT strips. METHODS: The POCT was used to test 170 serum specimens collected through measles surveillance or vaccination programmes in Ethiopia, Malaysia and the Russian Federation: 69 were positive for measles immunoglobulin M (IgM) antibodies, 74 were positive for rubella IgM antibodies and 7 were positive for both. Also tested were 282 oral fluid specimens from the measles, mumps and rubella (MMR) surveillance programme of the United Kingdom of Great Britain and Northern Ireland. The Microimmune measles IgM capture enzyme immunoassay was the gold standard for comparison. A panel of 24 oral fluids was used to investigate if measles virus haemagglutinin (H) and nucleocapsid (N) genes could be amplified by polymerase chain reaction directly from used POCT strips. FINDINGS: With serum POCT showed a sensitivity and specificity of 90.8% (69/76) and 93.6% (88/94), respectively; with oral fluids, sensitivity and specificity were 90.0% (63/70) and 96.2% (200/208), respectively. Both H and N genes were reliably detected in POCT strips and the N genes could be sequenced for genotyping. Measles virus genes could be recovered from POCT strips after storage for 5 weeks at 20-25 ºC. CONCLUSION: The POCT has the sensitivity and specificity required of a field-based test for measles diagnosis. However, its role in global measles control programmes requires further evaluation. |
| first_indexed | 2024-03-07T17:02:33Z |
| format | Article |
| id | doaj.art-1145d6c1ff2c40279b2512b4acf93bb3 |
| institution | Directory Open Access Journal |
| issn | 0042-9686 |
| language | English |
| last_indexed | 2024-03-07T17:02:33Z |
| publishDate | 2011-09-01 |
| publisher | The World Health Organization |
| record_format | Article |
| series | Bulletin of the World Health Organization |
| spelling | doaj.art-1145d6c1ff2c40279b2512b4acf93bb32024-03-03T03:14:27ZengThe World Health OrganizationBulletin of the World Health Organization0042-96862011-09-01899675682S0042-96862011000900014A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acidLenesha Warrener0Rimantas Slibinskas1Kaw Bing Chua2Wondatir Nigatu3Kevin E Brown4Kestutis Sasnauskas5Dhanraj Samuel6David Brown7Health Protection AgencyVilnius UniversityNational Public Health LaboratoryEthiopian Health and Nutrition Research InstituteHealth Protection AgencyVilnius UniversityHealth Protection AgencyHealth Protection AgencyOBJECTIVE: To evaluate the performance of a newly developed point-of-care test (POCT) for the detection of measles-specific IgM antibodies in serum and oral fluid specimens and to assess if measles virus nucleic acid could be recovered from used POCT strips. METHODS: The POCT was used to test 170 serum specimens collected through measles surveillance or vaccination programmes in Ethiopia, Malaysia and the Russian Federation: 69 were positive for measles immunoglobulin M (IgM) antibodies, 74 were positive for rubella IgM antibodies and 7 were positive for both. Also tested were 282 oral fluid specimens from the measles, mumps and rubella (MMR) surveillance programme of the United Kingdom of Great Britain and Northern Ireland. The Microimmune measles IgM capture enzyme immunoassay was the gold standard for comparison. A panel of 24 oral fluids was used to investigate if measles virus haemagglutinin (H) and nucleocapsid (N) genes could be amplified by polymerase chain reaction directly from used POCT strips. FINDINGS: With serum POCT showed a sensitivity and specificity of 90.8% (69/76) and 93.6% (88/94), respectively; with oral fluids, sensitivity and specificity were 90.0% (63/70) and 96.2% (200/208), respectively. Both H and N genes were reliably detected in POCT strips and the N genes could be sequenced for genotyping. Measles virus genes could be recovered from POCT strips after storage for 5 weeks at 20-25 ºC. CONCLUSION: The POCT has the sensitivity and specificity required of a field-based test for measles diagnosis. However, its role in global measles control programmes requires further evaluation.http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862011000900014&lng=en&tlng=en |
| spellingShingle | Lenesha Warrener Rimantas Slibinskas Kaw Bing Chua Wondatir Nigatu Kevin E Brown Kestutis Sasnauskas Dhanraj Samuel David Brown A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid Bulletin of the World Health Organization |
| title | A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid |
| title_full | A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid |
| title_fullStr | A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid |
| title_full_unstemmed | A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid |
| title_short | A point-of-care test for measles diagnosis: detection of measles-specific IgM antibodies and viral nucleic acid |
| title_sort | point of care test for measles diagnosis detection of measles specific igm antibodies and viral nucleic acid |
| url | http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S0042-96862011000900014&lng=en&tlng=en |
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