FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection
<p>Abstract</p> <p>Background</p> <p>Feline immunodeficiency virus (FIV) is a lentivirus of cats that establishes a lifelong persistent infection with immunologic impairment.</p> <p>Results</p> <p>In an approximately 2 year-long experimental infe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-02-01
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| Series: | Retrovirology |
| Subjects: | |
| Online Access: | http://www.retrovirology.com/content/9/1/12 |
| _version_ | 1811320745020620800 |
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| author | Murphy Brian Vapniarsky Natasha Hillman Chad Castillo Diego McDonnel Samantha Moore Peter Luciw Paul A Sparger Ellen E |
| author_facet | Murphy Brian Vapniarsky Natasha Hillman Chad Castillo Diego McDonnel Samantha Moore Peter Luciw Paul A Sparger Ellen E |
| author_sort | Murphy Brian |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Feline immunodeficiency virus (FIV) is a lentivirus of cats that establishes a lifelong persistent infection with immunologic impairment.</p> <p>Results</p> <p>In an approximately 2 year-long experimental infection study, cats infected with a biological isolate of FIV clade C demonstrated undetectable plasma viral loads from 10 months post-infection onward. Viral DNA was detected in CD4+CD25+ and CD4+CD25- T cells isolated from infected cats whereas viral RNA was not detected at multiple time points during the early chronic phase of infection. Viral transcription could be reactivated in latently infected CD4+ T cells <it>ex vivo </it>as demonstrated by detectable FIV <it>gag </it>RNA and 2-long terminal repeat (LTR) circle junctions. Viral LTR and <it>gag </it>sequences amplified from peripheral blood mononuclear cells during early and chronic stages of infection demonstrated minimal to no viral sequence variation.</p> <p>Conclusions</p> <p>Collectively, these findings are consistent with FIV latency in peripheral blood CD4+ T cells isolated from chronically infected cats. The ability to isolate latently FIV-infected CD4+ T lymphocytes from FIV-infected cats provides a platform for the study of <it>in vivo </it>mechanisms of lentiviral latency.</p> |
| first_indexed | 2024-04-13T13:05:54Z |
| format | Article |
| id | doaj.art-114927d413f4446f8ee5f6b014c03ac4 |
| institution | Directory Open Access Journal |
| issn | 1742-4690 |
| language | English |
| last_indexed | 2024-04-13T13:05:54Z |
| publishDate | 2012-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Retrovirology |
| spelling | doaj.art-114927d413f4446f8ee5f6b014c03ac42022-12-22T02:45:47ZengBMCRetrovirology1742-46902012-02-01911210.1186/1742-4690-9-12FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infectionMurphy BrianVapniarsky NatashaHillman ChadCastillo DiegoMcDonnel SamanthaMoore PeterLuciw Paul ASparger Ellen E<p>Abstract</p> <p>Background</p> <p>Feline immunodeficiency virus (FIV) is a lentivirus of cats that establishes a lifelong persistent infection with immunologic impairment.</p> <p>Results</p> <p>In an approximately 2 year-long experimental infection study, cats infected with a biological isolate of FIV clade C demonstrated undetectable plasma viral loads from 10 months post-infection onward. Viral DNA was detected in CD4+CD25+ and CD4+CD25- T cells isolated from infected cats whereas viral RNA was not detected at multiple time points during the early chronic phase of infection. Viral transcription could be reactivated in latently infected CD4+ T cells <it>ex vivo </it>as demonstrated by detectable FIV <it>gag </it>RNA and 2-long terminal repeat (LTR) circle junctions. Viral LTR and <it>gag </it>sequences amplified from peripheral blood mononuclear cells during early and chronic stages of infection demonstrated minimal to no viral sequence variation.</p> <p>Conclusions</p> <p>Collectively, these findings are consistent with FIV latency in peripheral blood CD4+ T cells isolated from chronically infected cats. The ability to isolate latently FIV-infected CD4+ T lymphocytes from FIV-infected cats provides a platform for the study of <it>in vivo </it>mechanisms of lentiviral latency.</p>http://www.retrovirology.com/content/9/1/12LentivirusFIVlatencyCD4+CD25+CD4+CD25-T cellmonocytecatfeline |
| spellingShingle | Murphy Brian Vapniarsky Natasha Hillman Chad Castillo Diego McDonnel Samantha Moore Peter Luciw Paul A Sparger Ellen E FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection Retrovirology Lentivirus FIV latency CD4+CD25+ CD4+CD25- T cell monocyte cat feline |
| title | FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection |
| title_full | FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection |
| title_fullStr | FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection |
| title_full_unstemmed | FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection |
| title_short | FIV establishes a latent infection in feline peripheral blood CD4+ T lymphocytes <it>in vivo </it>during the asymptomatic phase of infection |
| title_sort | fiv establishes a latent infection in feline peripheral blood cd4 t lymphocytes it in vivo it during the asymptomatic phase of infection |
| topic | Lentivirus FIV latency CD4+CD25+ CD4+CD25- T cell monocyte cat feline |
| url | http://www.retrovirology.com/content/9/1/12 |
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