Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes
Carbon-based nanomaterials (C-BNM) have recently attracted an increased attention as the materials with potential applications in industry and medicine. Bioresistance and proinflammatory potential of C-BNM is the main obstacle for their medicinal application which was documented in vivo and in vitro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/3/418 |
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| author | Tereza Svadlakova Frantisek Hubatka Pavlina Turanek Knotigova Pavel Kulich Josef Masek Jan Kotoucek Jan Macak Martin Motola Martin Kalbac Martina Kolackova Radka Vankova Petra Vicherkova Andrea Malkova Pavlina Simeckova Yuri Volkov Adriele Prina-Mello Irena Kratochvilova Zdenek Fiala Milan Raska Jan Krejsek Jaroslav Turanek |
| author_facet | Tereza Svadlakova Frantisek Hubatka Pavlina Turanek Knotigova Pavel Kulich Josef Masek Jan Kotoucek Jan Macak Martin Motola Martin Kalbac Martina Kolackova Radka Vankova Petra Vicherkova Andrea Malkova Pavlina Simeckova Yuri Volkov Adriele Prina-Mello Irena Kratochvilova Zdenek Fiala Milan Raska Jan Krejsek Jaroslav Turanek |
| author_sort | Tereza Svadlakova |
| collection | DOAJ |
| description | Carbon-based nanomaterials (C-BNM) have recently attracted an increased attention as the materials with potential applications in industry and medicine. Bioresistance and proinflammatory potential of C-BNM is the main obstacle for their medicinal application which was documented in vivo and in vitro. However, there are still limited data especially on graphene derivatives such as graphene platelets (GP). In this work, we compared multi-walled carbon nanotubes (MWCNT) and two different types of pristine GP in their potential to activate inflammasome NLRP3 (The nod-like receptor family pyrin domain containing 3) in vitro. Our study is focused on exposure of THP-1/THP1-null cells and peripheral blood monocytes to C-BNM as representative models of canonical and alternative pathways, respectively. Although all nanomaterials were extensively accumulated in the cytoplasm, increasing doses of all C-BNM did not lead to cell death. We observed direct activation of NLRP3 via destabilization of lysosomes and release of cathepsin B into cytoplasm only in the case of MWCNTs. Direct activation of NLRP3 by both GP was statistically insignificant but could be induced by synergic action with muramyl dipeptide (MDP), as a representative molecule of the family of pathogen-associated molecular patterns (PAMPs). This study demonstrates a possible proinflammatory potential of GP and MWCNT acting through NLRP3 activation. |
| first_indexed | 2024-12-11T22:16:01Z |
| format | Article |
| id | doaj.art-114f102ef03e4e7aa29cafe4450ead35 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-11T22:16:01Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-114f102ef03e4e7aa29cafe4450ead352022-12-22T00:48:36ZengMDPI AGNanomaterials2079-49912020-02-0110341810.3390/nano10030418nano10030418Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of LysosomesTereza Svadlakova0Frantisek Hubatka1Pavlina Turanek Knotigova2Pavel Kulich3Josef Masek4Jan Kotoucek5Jan Macak6Martin Motola7Martin Kalbac8Martina Kolackova9Radka Vankova10Petra Vicherkova11Andrea Malkova12Pavlina Simeckova13Yuri Volkov14Adriele Prina-Mello15Irena Kratochvilova16Zdenek Fiala17Milan Raska18Jan Krejsek19Jaroslav Turanek20Institute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicCenter of Materials and Nanotechnologies, Faculty of Chemical Technology, University of Pardubice, 53002 Pardubice, Czech RepublicCenter of Materials and Nanotechnologies, Faculty of Chemical Technology, University of Pardubice, 53002 Pardubice, Czech RepublicJ. Heyrovsky Institute of Physical Chemistry of the Czech Academy of Sciences, 18223 Prague, Czech RepublicInstitute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech RepublicInstitute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech RepublicInstitute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech RepublicInstitute of Hygiene and Preventive Medicine, Faculty of Medicine in Hradec Kralove, Charles University, 50003 Hradec Kralove, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicDepartment of Clinical Medicine/Trinity Translational Medicine Institute (TTMI), Trinity College Dublin, D08 W9RT, Dublin, IrelandDepartment of Clinical Medicine/Trinity Translational Medicine Institute (TTMI), Trinity College Dublin, D08 W9RT, Dublin, IrelandInstitute of Physics, Czech Academy of Sciences, 18200 Prague, Czech RepublicInstitute of Hygiene and Preventive Medicine, Faculty of Medicine in Hradec Kralove, Charles University, 50003 Hradec Kralove, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicInstitute of Clinical Immunology and Allergology, University Hospital Hradec Kralove and Faculty of Medicine in Hradec Kralove, Charles University, 50005 Hradec Kralove, Czech RepublicVeterinary Research Institute, 62100 Brno, Czech RepublicCarbon-based nanomaterials (C-BNM) have recently attracted an increased attention as the materials with potential applications in industry and medicine. Bioresistance and proinflammatory potential of C-BNM is the main obstacle for their medicinal application which was documented in vivo and in vitro. However, there are still limited data especially on graphene derivatives such as graphene platelets (GP). In this work, we compared multi-walled carbon nanotubes (MWCNT) and two different types of pristine GP in their potential to activate inflammasome NLRP3 (The nod-like receptor family pyrin domain containing 3) in vitro. Our study is focused on exposure of THP-1/THP1-null cells and peripheral blood monocytes to C-BNM as representative models of canonical and alternative pathways, respectively. Although all nanomaterials were extensively accumulated in the cytoplasm, increasing doses of all C-BNM did not lead to cell death. We observed direct activation of NLRP3 via destabilization of lysosomes and release of cathepsin B into cytoplasm only in the case of MWCNTs. Direct activation of NLRP3 by both GP was statistically insignificant but could be induced by synergic action with muramyl dipeptide (MDP), as a representative molecule of the family of pathogen-associated molecular patterns (PAMPs). This study demonstrates a possible proinflammatory potential of GP and MWCNT acting through NLRP3 activation.https://www.mdpi.com/2079-4991/10/3/418graphene plateletscarbon nanotubesinflammasome nlrp3cathepsin bmacrophagesthp-1 |
| spellingShingle | Tereza Svadlakova Frantisek Hubatka Pavlina Turanek Knotigova Pavel Kulich Josef Masek Jan Kotoucek Jan Macak Martin Motola Martin Kalbac Martina Kolackova Radka Vankova Petra Vicherkova Andrea Malkova Pavlina Simeckova Yuri Volkov Adriele Prina-Mello Irena Kratochvilova Zdenek Fiala Milan Raska Jan Krejsek Jaroslav Turanek Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes Nanomaterials graphene platelets carbon nanotubes inflammasome nlrp3 cathepsin b macrophages thp-1 |
| title | Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes |
| title_full | Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes |
| title_fullStr | Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes |
| title_full_unstemmed | Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes |
| title_short | Proinflammatory Effect of Carbon-Based Nanomaterials: In Vitro Study on Stimulation of Inflammasome NLRP3 via Destabilisation of Lysosomes |
| title_sort | proinflammatory effect of carbon based nanomaterials in vitro study on stimulation of inflammasome nlrp3 via destabilisation of lysosomes |
| topic | graphene platelets carbon nanotubes inflammasome nlrp3 cathepsin b macrophages thp-1 |
| url | https://www.mdpi.com/2079-4991/10/3/418 |
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