Phytochemical characterization by GC-MS and in vitro evaluation of antiproliferative and antimigratory studies of Leucas aspera leaf extracts on MDA-MB-231 cell line
Breast cancer is the most recurrently identified and one of women’s prominent causes of death. Currently, researchers have turned their focus on natural chemicals from synthetic chemicals due to their environmental, economic, and health benefits. Considering this, the medicinal plant Leucas aspera...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2024-03-01
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| Series: | BioTechnologia |
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| Online Access: | https://www.termedia.pl/Phytochemical-characterization-by-GC-MS-r-nand-in-vitro-evaluation-of-antiproliferative-r-nand-antimigratory-studies-of-Leucas-aspera-leaf-extracts-r-non-MDA-MB-231-cell-line,85,52457,1,1.html |
| _version_ | 1797214465689649152 |
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| author | Fazeela Mahaboob Begum S.M Megasri Sankarram |
| author_facet | Fazeela Mahaboob Begum S.M Megasri Sankarram |
| author_sort | Fazeela Mahaboob Begum S.M |
| collection | DOAJ |
| description | Breast cancer is the most recurrently identified and one of women’s prominent causes of death. Currently, researchers have turned their focus on natural chemicals from synthetic chemicals due to their environmental, economic, and health benefits. Considering this, the medicinal plant Leucas aspera was chosen for the current study. The aim of this study was to isolate and characterize secondary metabolites from L. aspera and determine the antiproliferative and antimigratory activities in the MDA-MB-231 cell line under in vitro conditions. Phytochemicals from L. aspera were isolated through sequential extraction using hexane, dichloromethane, and ethyl acetate. These extracts were qualitatively screened, subjected to FT-IR, and analyzed using GC-MS. The antiproliferative activity was determined through the MTT assay. Scratch assay was utilized to determine the antimigratory activity of the plant extracts. The phytochemical analysis revealed the presence of steroids, alkaloids, phenols, flavonoids, galactose, tannins, saponins, and amino acids in the extracts. The results of the cell viability assay indicated that the crude dichloromethane and ethyl acetate extracts inhibited cell proliferation, with inhibitory concentrations of 5 and 3 μg/ml, respectively. In contrast, the crude hexane extract did not exhibit any cytotoxicity. Furthermore, the scratch assay results showed that the plant extracts had cell migration inhibitory properties. The outcomes of the current study conclude that L. aspera possesses active therapeutic agents with strong anticancer potential, effectively impeding the proliferation and invasion of MDA-MB-231. Further studies are needed to identify the potential active agents that contribute to these activities. |
| first_indexed | 2024-04-24T11:14:36Z |
| format | Article |
| id | doaj.art-1151253db5854a868a583981cb06755a |
| institution | Directory Open Access Journal |
| issn | 0860-7796 2353-9461 |
| language | English |
| last_indexed | 2024-04-24T11:14:36Z |
| publishDate | 2024-03-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | BioTechnologia |
| spelling | doaj.art-1151253db5854a868a583981cb06755a2024-04-11T09:07:59ZengTermedia Publishing HouseBioTechnologia0860-77962353-94612024-03-011051556810.5114/bta.2024.13564252457Phytochemical characterization by GC-MS and in vitro evaluation of antiproliferative and antimigratory studies of Leucas aspera leaf extracts on MDA-MB-231 cell lineFazeela Mahaboob Begum S.MMegasri SankarramBreast cancer is the most recurrently identified and one of women’s prominent causes of death. Currently, researchers have turned their focus on natural chemicals from synthetic chemicals due to their environmental, economic, and health benefits. Considering this, the medicinal plant Leucas aspera was chosen for the current study. The aim of this study was to isolate and characterize secondary metabolites from L. aspera and determine the antiproliferative and antimigratory activities in the MDA-MB-231 cell line under in vitro conditions. Phytochemicals from L. aspera were isolated through sequential extraction using hexane, dichloromethane, and ethyl acetate. These extracts were qualitatively screened, subjected to FT-IR, and analyzed using GC-MS. The antiproliferative activity was determined through the MTT assay. Scratch assay was utilized to determine the antimigratory activity of the plant extracts. The phytochemical analysis revealed the presence of steroids, alkaloids, phenols, flavonoids, galactose, tannins, saponins, and amino acids in the extracts. The results of the cell viability assay indicated that the crude dichloromethane and ethyl acetate extracts inhibited cell proliferation, with inhibitory concentrations of 5 and 3 μg/ml, respectively. In contrast, the crude hexane extract did not exhibit any cytotoxicity. Furthermore, the scratch assay results showed that the plant extracts had cell migration inhibitory properties. The outcomes of the current study conclude that L. aspera possesses active therapeutic agents with strong anticancer potential, effectively impeding the proliferation and invasion of MDA-MB-231. Further studies are needed to identify the potential active agents that contribute to these activities.https://www.termedia.pl/Phytochemical-characterization-by-GC-MS-r-nand-in-vitro-evaluation-of-antiproliferative-r-nand-antimigratory-studies-of-Leucas-aspera-leaf-extracts-r-non-MDA-MB-231-cell-line,85,52457,1,1.htmlcytotoxic activity mda-mb-231 breast cancer leucas aspera anti-migratory efficiency |
| spellingShingle | Fazeela Mahaboob Begum S.M Megasri Sankarram Phytochemical characterization by GC-MS and in vitro evaluation of antiproliferative and antimigratory studies of Leucas aspera leaf extracts on MDA-MB-231 cell line BioTechnologia cytotoxic activity mda-mb-231 breast cancer leucas aspera anti-migratory efficiency |
| title | Phytochemical characterization by GC-MS
and in vitro evaluation of antiproliferative
and antimigratory studies of Leucas aspera leaf extracts
on MDA-MB-231 cell line |
| title_full | Phytochemical characterization by GC-MS
and in vitro evaluation of antiproliferative
and antimigratory studies of Leucas aspera leaf extracts
on MDA-MB-231 cell line |
| title_fullStr | Phytochemical characterization by GC-MS
and in vitro evaluation of antiproliferative
and antimigratory studies of Leucas aspera leaf extracts
on MDA-MB-231 cell line |
| title_full_unstemmed | Phytochemical characterization by GC-MS
and in vitro evaluation of antiproliferative
and antimigratory studies of Leucas aspera leaf extracts
on MDA-MB-231 cell line |
| title_short | Phytochemical characterization by GC-MS
and in vitro evaluation of antiproliferative
and antimigratory studies of Leucas aspera leaf extracts
on MDA-MB-231 cell line |
| title_sort | phytochemical characterization by gc ms and in vitro evaluation of antiproliferative and antimigratory studies of leucas aspera leaf extracts on mda mb 231 cell line |
| topic | cytotoxic activity mda-mb-231 breast cancer leucas aspera anti-migratory efficiency |
| url | https://www.termedia.pl/Phytochemical-characterization-by-GC-MS-r-nand-in-vitro-evaluation-of-antiproliferative-r-nand-antimigratory-studies-of-Leucas-aspera-leaf-extracts-r-non-MDA-MB-231-cell-line,85,52457,1,1.html |
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