Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology
Human Chagas' disease, caused by the protozoan Trypanosoma cruzi, is associated with pathological processes whose mechanisms are not known. To address this question, T cell lines were developed from chronic chagasic patients peripheral blood mononuclear cells (PBMC) and cloned. These T cell clo...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1995-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000400013 |
| _version_ | 1797718270858493952 |
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| author | Washington R. Cuna Celeste Rodriguez Cuna |
| author_facet | Washington R. Cuna Celeste Rodriguez Cuna |
| author_sort | Washington R. Cuna |
| collection | DOAJ |
| description | Human Chagas' disease, caused by the protozoan Trypanosoma cruzi, is associated with pathological processes whose mechanisms are not known. To address this question, T cell lines were developed from chronic chagasic patients peripheral blood mononuclear cells (PBMC) and cloned. These T cell clones (TCC) were analyzed phenotypically with monoclonal antibodies by the use of a fluorescence microscope. The surface phenotype of the TCC from the asymptomatic patient were predominantly CD4 positive (86%). On the contrary, the surface phenotype CD8 was predominant in the TCC from the patients suffering from cardiomegaly with right bundle branch block (83%), bradycardia with megacolon (75 %) and bradycardia (75%). Future studies will be developed in order to identify the antigens eliciting these T cell subpopulations. |
| first_indexed | 2024-03-12T08:48:26Z |
| format | Article |
| id | doaj.art-115125891d9f42989e2ac99deec590a4 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:48:26Z |
| publishDate | 1995-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-115125891d9f42989e2ac99deec590a42023-09-02T16:22:39ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601995-08-0190450350610.1590/S0074-02761995000400013Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathologyWashington R. CunaCeleste Rodriguez CunaHuman Chagas' disease, caused by the protozoan Trypanosoma cruzi, is associated with pathological processes whose mechanisms are not known. To address this question, T cell lines were developed from chronic chagasic patients peripheral blood mononuclear cells (PBMC) and cloned. These T cell clones (TCC) were analyzed phenotypically with monoclonal antibodies by the use of a fluorescence microscope. The surface phenotype of the TCC from the asymptomatic patient were predominantly CD4 positive (86%). On the contrary, the surface phenotype CD8 was predominant in the TCC from the patients suffering from cardiomegaly with right bundle branch block (83%), bradycardia with megacolon (75 %) and bradycardia (75%). Future studies will be developed in order to identify the antigens eliciting these T cell subpopulations.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000400013Trypanosoma cruziT cell clonesasymptomaticpathology |
| spellingShingle | Washington R. Cuna Celeste Rodriguez Cuna Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology Memorias do Instituto Oswaldo Cruz Trypanosoma cruzi T cell clones asymptomatic pathology |
| title | Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology |
| title_full | Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology |
| title_fullStr | Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology |
| title_full_unstemmed | Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology |
| title_short | Characterization of T cell clones from chagasic patients: predominance of CD8 surface phenotype in clones from patients with pathology |
| title_sort | characterization of t cell clones from chagasic patients predominance of cd8 surface phenotype in clones from patients with pathology |
| topic | Trypanosoma cruzi T cell clones asymptomatic pathology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761995000400013 |
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