The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells
Ginkgolide B, the major active component of Ginkgo biloba extracts, can bothstimulate and inhibit apoptotic signaling. Here, we demonstrate that ginkgolide B caninduce the production of reactive oxygen species in MCF-7 breast cancer cells, leading toan increase in the intracellular concentrations of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2007-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/8/11/1177/ |
| _version_ | 1811315876209623040 |
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| author | Wen-Hsiung Chan |
| author_facet | Wen-Hsiung Chan |
| author_sort | Wen-Hsiung Chan |
| collection | DOAJ |
| description | Ginkgolide B, the major active component of Ginkgo biloba extracts, can bothstimulate and inhibit apoptotic signaling. Here, we demonstrate that ginkgolide B caninduce the production of reactive oxygen species in MCF-7 breast cancer cells, leading toan increase in the intracellular concentrations of cytoplasmic free Ca2+ and nitric oxide(NO), loss of mitochondrial membrane potential (MMP), activation of caspase-9 and -3,and increase the mRNA expression levels of p53 and p21, which are known to be involvedin apoptotic signaling. In addition, prevention of ROS generation by pretreatment withN-acetyl cysteine (NAC) could effectively block intracellular Ca2+ concentrationsincreases and apoptosis in ginkgolide B-treated MCF-7 cells. Moreover, pretreatment withnitric oxide (NO) scavengers could inhibit ginkgolide B-induced MMP change andsequent apoptotic processes. Overall, our results signify that both ROS and NO playedimportant roles in ginkgolide B-induced apoptosis of MCF-7 cells. Based on these studyresults, we propose a model for ginkgolide B-induced cell apoptosis signaling cascades inMCF-7 cells. |
| first_indexed | 2024-04-13T11:39:12Z |
| format | Article |
| id | doaj.art-115639ed9b5e46caa82362be6549ba6a |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T11:39:12Z |
| publishDate | 2007-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-115639ed9b5e46caa82362be6549ba6a2022-12-22T02:48:21ZengMDPI AGInternational Journal of Molecular Sciences1422-00672007-11-018111177119510.3390/i8111177The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer CellsWen-Hsiung ChanGinkgolide B, the major active component of Ginkgo biloba extracts, can bothstimulate and inhibit apoptotic signaling. Here, we demonstrate that ginkgolide B caninduce the production of reactive oxygen species in MCF-7 breast cancer cells, leading toan increase in the intracellular concentrations of cytoplasmic free Ca2+ and nitric oxide(NO), loss of mitochondrial membrane potential (MMP), activation of caspase-9 and -3,and increase the mRNA expression levels of p53 and p21, which are known to be involvedin apoptotic signaling. In addition, prevention of ROS generation by pretreatment withN-acetyl cysteine (NAC) could effectively block intracellular Ca2+ concentrationsincreases and apoptosis in ginkgolide B-treated MCF-7 cells. Moreover, pretreatment withnitric oxide (NO) scavengers could inhibit ginkgolide B-induced MMP change andsequent apoptotic processes. Overall, our results signify that both ROS and NO playedimportant roles in ginkgolide B-induced apoptosis of MCF-7 cells. Based on these studyresults, we propose a model for ginkgolide B-induced cell apoptosis signaling cascades inMCF-7 cells.http://www.mdpi.com/1422-0067/8/11/1177/GinkgolideApoptosisOxidative StressNitric oxide |
| spellingShingle | Wen-Hsiung Chan The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells International Journal of Molecular Sciences Ginkgolide Apoptosis Oxidative Stress Nitric oxide |
| title | The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells |
| title_full | The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells |
| title_fullStr | The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells |
| title_full_unstemmed | The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells |
| title_short | The Signaling Cascades of Ginkgolide B-Induced Apoptosis in MCF-7 Breast Cancer Cells |
| title_sort | signaling cascades of ginkgolide b induced apoptosis in mcf 7 breast cancer cells |
| topic | Ginkgolide Apoptosis Oxidative Stress Nitric oxide |
| url | http://www.mdpi.com/1422-0067/8/11/1177/ |
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