Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract
In order to investigate the anti-photoaging effect and mechanism of tomato and aronia extracts, the effects of tomato extract and aronia extract on apoptosis, reactive oxygen species (ROS) and prostaglandin E2 (PGE2) were studied in UVB-irradiated HaCaT cells. Hematoxylin-eosin (HE) staining was use...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090229 |
| _version_ | 1827861224675606528 |
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| author | Zhen LUO Chuguang NING Zuozhen LI Chunyan YAO Xiaoli WEI Hongying LI Ya CAI Shandong JIANG |
| author_facet | Zhen LUO Chuguang NING Zuozhen LI Chunyan YAO Xiaoli WEI Hongying LI Ya CAI Shandong JIANG |
| author_sort | Zhen LUO |
| collection | DOAJ |
| description | In order to investigate the anti-photoaging effect and mechanism of tomato and aronia extracts, the effects of tomato extract and aronia extract on apoptosis, reactive oxygen species (ROS) and prostaglandin E2 (PGE2) were studied in UVB-irradiated HaCaT cells. Hematoxylin-eosin (HE) staining was used to evaluate the anti-photoaging effect of tomato and aronia complex (LA) on skin photoaging in UVB-irradiated ICR mice. The activity of superoxide dismutase (SOD), the level of malondialdehyde (MDA) and the expression of pro-inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) were detected. The results showed that tomato extract and aronia extract were not cytotoxic to HaCaT cells. In the photoaging model of HaCaT cells, compared with the model group, the number of apoptotic cells in 100 μg/mL tomato extract group and 30μg/mL aronia extract group were significantly decreased (P<0.05). Aronia extract (100 μg/mL) significantly decreased ROS level (P<0.01). At 100 μg/mL, PEG2 was significantly decreased in both tomato and aronia extract groups (P<0.05). Compared with the model group, LA treatment group resulted in a significant reduction in dermal thickness (P<0.01) and MDA levels (P<0.01), and a significant increase in SOD activity (P<0.01) in skin tissue, while the proinflammatory factors IL-6, COX-2 and TNF-α were all significantly decreased (P<0.05). Tomato and aronia complex can improve oxidative stress and inflammation induced by UVB, and has the effect of anti-photoaging. |
| first_indexed | 2024-03-12T13:35:05Z |
| format | Article |
| id | doaj.art-1159e4b976724cbfb98a5207ddf9bdb0 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:35:05Z |
| publishDate | 2023-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-1159e4b976724cbfb98a5207ddf9bdb02023-08-24T06:00:02ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-08-01441639540210.13386/j.issn1002-0306.20220902292022090229-16Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa ExtractZhen LUO0Chuguang NING1Zuozhen LI2Chunyan YAO3Xiaoli WEI4Hongying LI5Ya CAI6Shandong JIANG7Infinitus (China) Company Ltd., Guangzhou 510623, ChinaInfinitus (China) Company Ltd., Guangzhou 510623, ChinaInfinitus (China) Company Ltd., Guangzhou 510623, ChinaHangzhou Medical College, Hangzhou 310013, ChinaHealthy-Star Biological Technology Research and Development (Shanghai) Co., Ltd., Shanghai 200335, China)Healthy-Star Biological Technology Research and Development (Shanghai) Co., Ltd., Shanghai 200335, China)Healthy-Star Biological Technology Research and Development (Shanghai) Co., Ltd., Shanghai 200335, China)Hangzhou Medical College, Hangzhou 310013, ChinaIn order to investigate the anti-photoaging effect and mechanism of tomato and aronia extracts, the effects of tomato extract and aronia extract on apoptosis, reactive oxygen species (ROS) and prostaglandin E2 (PGE2) were studied in UVB-irradiated HaCaT cells. Hematoxylin-eosin (HE) staining was used to evaluate the anti-photoaging effect of tomato and aronia complex (LA) on skin photoaging in UVB-irradiated ICR mice. The activity of superoxide dismutase (SOD), the level of malondialdehyde (MDA) and the expression of pro-inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α) were detected. The results showed that tomato extract and aronia extract were not cytotoxic to HaCaT cells. In the photoaging model of HaCaT cells, compared with the model group, the number of apoptotic cells in 100 μg/mL tomato extract group and 30μg/mL aronia extract group were significantly decreased (P<0.05). Aronia extract (100 μg/mL) significantly decreased ROS level (P<0.01). At 100 μg/mL, PEG2 was significantly decreased in both tomato and aronia extract groups (P<0.05). Compared with the model group, LA treatment group resulted in a significant reduction in dermal thickness (P<0.01) and MDA levels (P<0.01), and a significant increase in SOD activity (P<0.01) in skin tissue, while the proinflammatory factors IL-6, COX-2 and TNF-α were all significantly decreased (P<0.05). Tomato and aronia complex can improve oxidative stress and inflammation induced by UVB, and has the effect of anti-photoaging.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090229tomatoaronia melanocarpaskinuvbphotoaging |
| spellingShingle | Zhen LUO Chuguang NING Zuozhen LI Chunyan YAO Xiaoli WEI Hongying LI Ya CAI Shandong JIANG Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract Shipin gongye ke-ji tomato aronia melanocarpa skin uvb photoaging |
| title | Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract |
| title_full | Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract |
| title_fullStr | Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract |
| title_full_unstemmed | Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract |
| title_short | Anti-photoaging Effect and Mechanism of Tomato and Aronia melanocarpa Extract |
| title_sort | anti photoaging effect and mechanism of tomato and aronia melanocarpa extract |
| topic | tomato aronia melanocarpa skin uvb photoaging |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022090229 |
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