Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i>
The unpropitious conditions of the food processing environment trigger in <i>Listeria monocytogenes</i> stress response mechanisms that may affect the pathogen’s virulence. To date, many studies have revealed that acid, osmotic, heat, cold and oxidative stress modify invasivene...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/10/467 |
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| author | Ewa Wałecka-Zacharska Jakub Korkus Krzysztof Skowron Magdalena Wietlicka-Piszcz Katarzyna Kosek-Paszkowska Jacek Bania |
| author_facet | Ewa Wałecka-Zacharska Jakub Korkus Krzysztof Skowron Magdalena Wietlicka-Piszcz Katarzyna Kosek-Paszkowska Jacek Bania |
| author_sort | Ewa Wałecka-Zacharska |
| collection | DOAJ |
| description | The unpropitious conditions of the food processing environment trigger in <i>Listeria monocytogenes</i> stress response mechanisms that may affect the pathogen’s virulence. To date, many studies have revealed that acid, osmotic, heat, cold and oxidative stress modify invasiveness of <i>L. monocytogenes</i>. Nonetheless, there is limited data on the duration of the stress effect on bacterial invasiveness. Since most food is stored at low or room temperatures we studied the impact of these temperatures on the duration of heat stress effect on invasiveness of 8 <i>L. monocytogenes</i> strains. Bacteria were heat-treated for 20 min at 54 °C and then incubated at 5 and 20 °C up to 14 days. A decrease in invasiveness over time was observed for bacteria not exposed to heating. It was found that heat shock significantly reduced the invasion capacity of all strains and the effect lasted between 7 and 14 days at both 5 and 20 °C. In conclusion, 20-min heating reduces invasion capacity of all <i>L. monocytogenes</i> strains; however, the stress effect is temporary and lasts between 7 and 14 days in the food storage conditions. The invasiveness of bacteria changes along with the incubation time and is temperature-dependent. |
| first_indexed | 2024-04-12T02:56:55Z |
| format | Article |
| id | doaj.art-115a1b33ae284756b690c954a5997d20 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-12T02:56:55Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-115a1b33ae284756b690c954a5997d202022-12-22T03:50:47ZengMDPI AGMicroorganisms2076-26072019-10-0171046710.3390/microorganisms7100467microorganisms7100467Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i>Ewa Wałecka-Zacharska0Jakub Korkus1Krzysztof Skowron2Magdalena Wietlicka-Piszcz3Katarzyna Kosek-Paszkowska4Jacek Bania5Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandDepartment of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandDepartment of Microbiology, Nicolaus Copernicus University in Toruń, Collegium Medicum of L. Rydygier in Bydgoszcz, 85-094 Bydgoszcz, PolandDepartment of Theoretical Foundations of Biomedical Sciences and Medical Computer Science, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, 9 M. Skłodowska-Curie St., 85-094 Bydgoszcz, PolandDepartment of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandDepartment of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, PolandThe unpropitious conditions of the food processing environment trigger in <i>Listeria monocytogenes</i> stress response mechanisms that may affect the pathogen’s virulence. To date, many studies have revealed that acid, osmotic, heat, cold and oxidative stress modify invasiveness of <i>L. monocytogenes</i>. Nonetheless, there is limited data on the duration of the stress effect on bacterial invasiveness. Since most food is stored at low or room temperatures we studied the impact of these temperatures on the duration of heat stress effect on invasiveness of 8 <i>L. monocytogenes</i> strains. Bacteria were heat-treated for 20 min at 54 °C and then incubated at 5 and 20 °C up to 14 days. A decrease in invasiveness over time was observed for bacteria not exposed to heating. It was found that heat shock significantly reduced the invasion capacity of all strains and the effect lasted between 7 and 14 days at both 5 and 20 °C. In conclusion, 20-min heating reduces invasion capacity of all <i>L. monocytogenes</i> strains; however, the stress effect is temporary and lasts between 7 and 14 days in the food storage conditions. The invasiveness of bacteria changes along with the incubation time and is temperature-dependent.https://www.mdpi.com/2076-2607/7/10/467<i>listeria monocytogenes</i>heat stressinvasivenessfood storage temperature |
| spellingShingle | Ewa Wałecka-Zacharska Jakub Korkus Krzysztof Skowron Magdalena Wietlicka-Piszcz Katarzyna Kosek-Paszkowska Jacek Bania Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i> Microorganisms <i>listeria monocytogenes</i> heat stress invasiveness food storage temperature |
| title | Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i> |
| title_full | Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i> |
| title_fullStr | Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i> |
| title_full_unstemmed | Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i> |
| title_short | Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness of <i>L. monocytogenes</i> |
| title_sort | effect of temperatures used in food storage on duration of heat stress induced invasiveness of i l monocytogenes i |
| topic | <i>listeria monocytogenes</i> heat stress invasiveness food storage temperature |
| url | https://www.mdpi.com/2076-2607/7/10/467 |
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