Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran
<p>Abstract</p> <p>Background</p> <p>This work was carried out to assess the patterns and prevalence of resistance to chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) in Iran.</p> <p>Methods</p> <p>The prevalence of <it>pfcrt </it>...
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| Format: | Article |
| Language: | English |
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BMC
2007-11-01
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| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/6/1/148 |
| _version_ | 1811245022827249664 |
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| author | Djadid Navid Raeisi Ahmad Afsharpad Mandana Zakeri Sedigheh |
| author_facet | Djadid Navid Raeisi Ahmad Afsharpad Mandana Zakeri Sedigheh |
| author_sort | Djadid Navid |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>This work was carried out to assess the patterns and prevalence of resistance to chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) in Iran.</p> <p>Methods</p> <p>The prevalence of <it>pfcrt </it>K76T, <it>pfmdr1 </it>N86Y, <it>pfdhfr </it>N51I, C59R, S108N/T and I164L and codons S436F/A, A437G, K540E, A581E, and A613S/T in <it>pfdhps </it>genes were genotyped by PCR/RFLP methods in 206 <it>Plasmodium falciparum </it>isolates from Chabahar and Sarbaz districts in Sistan and Baluchistan province, Iran, during 2003–2005.</p> <p>Results</p> <p>All <it>P. falciparum </it>isolates carried the 108N, while 98.5% parasite isolates carried the 59R mutation. 98.5% of patients carried both 108N and 59R. The prevalence of <it>pfdhps </it>437G mutation was 17% (Chabahar) and 33% (Sarbaz) isolates. 20.4% of samples presented the <it>pfdhfr </it>108N, 59R with <it>pfdhps </it>437G mutations. The frequency of allele <it>pfcrt </it>76T was 98%, while 41.4% (Chabahar) and 27.7% (Sarbaz) isolates carried <it>pfmdr1 </it>86Y allele. Eight distinct haplotypes were identified in all 206 samples, while the most prevalent haplotype was <b>T</b><sub>76/</sub>N<sub>86/</sub>N<sub>51</sub><b>R</b><sub>59</sub><b>N</b><sub>108/</sub>A<sub>437 </sub>among both study areas.</p> <p>Conclusion</p> <p>Finding the fixed level of CQ resistance polymorphisms (<it>pfcrt </it>76T) suggests that CQ must be withdrawn from the current treatment strategy in Iran, while SP may remain the treatment of choice for uncomplicated malaria.</p> |
| first_indexed | 2024-04-12T14:33:54Z |
| format | Article |
| id | doaj.art-1163fee263954709bb9129e2b6a0e984 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-12T14:33:54Z |
| publishDate | 2007-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-1163fee263954709bb9129e2b6a0e9842022-12-22T03:29:09ZengBMCMalaria Journal1475-28752007-11-016114810.1186/1475-2875-6-148Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in IranDjadid NavidRaeisi AhmadAfsharpad MandanaZakeri Sedigheh<p>Abstract</p> <p>Background</p> <p>This work was carried out to assess the patterns and prevalence of resistance to chloroquine (CQ) and sulphadoxine-pyrimethamine (SP) in Iran.</p> <p>Methods</p> <p>The prevalence of <it>pfcrt </it>K76T, <it>pfmdr1 </it>N86Y, <it>pfdhfr </it>N51I, C59R, S108N/T and I164L and codons S436F/A, A437G, K540E, A581E, and A613S/T in <it>pfdhps </it>genes were genotyped by PCR/RFLP methods in 206 <it>Plasmodium falciparum </it>isolates from Chabahar and Sarbaz districts in Sistan and Baluchistan province, Iran, during 2003–2005.</p> <p>Results</p> <p>All <it>P. falciparum </it>isolates carried the 108N, while 98.5% parasite isolates carried the 59R mutation. 98.5% of patients carried both 108N and 59R. The prevalence of <it>pfdhps </it>437G mutation was 17% (Chabahar) and 33% (Sarbaz) isolates. 20.4% of samples presented the <it>pfdhfr </it>108N, 59R with <it>pfdhps </it>437G mutations. The frequency of allele <it>pfcrt </it>76T was 98%, while 41.4% (Chabahar) and 27.7% (Sarbaz) isolates carried <it>pfmdr1 </it>86Y allele. Eight distinct haplotypes were identified in all 206 samples, while the most prevalent haplotype was <b>T</b><sub>76/</sub>N<sub>86/</sub>N<sub>51</sub><b>R</b><sub>59</sub><b>N</b><sub>108/</sub>A<sub>437 </sub>among both study areas.</p> <p>Conclusion</p> <p>Finding the fixed level of CQ resistance polymorphisms (<it>pfcrt </it>76T) suggests that CQ must be withdrawn from the current treatment strategy in Iran, while SP may remain the treatment of choice for uncomplicated malaria.</p>http://www.malariajournal.com/content/6/1/148 |
| spellingShingle | Djadid Navid Raeisi Ahmad Afsharpad Mandana Zakeri Sedigheh Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran Malaria Journal |
| title | Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran |
| title_full | Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran |
| title_fullStr | Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran |
| title_full_unstemmed | Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran |
| title_short | Prevalence of mutations associated with antimalarial drugs in <it>Plasmodium falciparum </it>isolates prior to the introduction of sulphadoxine-pyrimethamine as first-line treatment in Iran |
| title_sort | prevalence of mutations associated with antimalarial drugs in it plasmodium falciparum it isolates prior to the introduction of sulphadoxine pyrimethamine as first line treatment in iran |
| url | http://www.malariajournal.com/content/6/1/148 |
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