Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples
In this work, a flow-based spectrofluorimetric method for iodine determination was developed. The system consisted of a miniaturized chip-based flow manifold for solutions handling and with integrated spectrofluorimetric detection. A multi-syringe module was used as a liquid driver. Iodide was quant...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1325 |
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| author | Joana L. A. Miranda Raquel B. R. Mesquita Edwin Palacio José M. Estela Víctor Cerdà António O. S. S. Rangel |
| author_facet | Joana L. A. Miranda Raquel B. R. Mesquita Edwin Palacio José M. Estela Víctor Cerdà António O. S. S. Rangel |
| author_sort | Joana L. A. Miranda |
| collection | DOAJ |
| description | In this work, a flow-based spectrofluorimetric method for iodine determination was developed. The system consisted of a miniaturized chip-based flow manifold for solutions handling and with integrated spectrofluorimetric detection. A multi-syringe module was used as a liquid driver. Iodide was quantified from its catalytic effect on the redox reaction between Ce(IV) and As(III), based on the Sandell–Kolthoff reaction. The method was applied for the determination of iodine in salt, pharmaceuticals, supplement pills, and seaweed samples without off-line pre-treatment. An in-line oxidation process, aided by UV radiation, was implemented to analyse some samples (supplement pills and seaweed samples) to eliminate interferences and release iodine from organo-iodine compounds. This feature, combined with the fluorometric reaction, makes this method simpler, faster, and more sensitive than the classic approach of the Sandell–Kolthoff reaction. The method allowed iodine to be determined within a range of 0.20–4.0 µmol L<sup>−1</sup>, with or without the in-line UV digestion, with a limit of detection of 0.028 µmol L<sup>−1</sup> and 0.025 µmol L<sup>−1</sup>, respectively. |
| first_indexed | 2024-03-09T21:20:29Z |
| format | Article |
| id | doaj.art-1168ff449e2d40afaaae1f832dd2c8c2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:20:29Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1168ff449e2d40afaaae1f832dd2c8c22023-11-23T21:22:14ZengMDPI AGMolecules1420-30492022-02-01274132510.3390/molecules27041325Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae SamplesJoana L. A. Miranda0Raquel B. R. Mesquita1Edwin Palacio2José M. Estela3Víctor Cerdà4António O. S. S. Rangel5CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalDepartment of Chemistry, University of the Balearic Islands, 07122 Palma de Mallorca, SpainDepartment of Chemistry, University of the Balearic Islands, 07122 Palma de Mallorca, SpainSciware Systems S.L., 07193 Bunyola, SpainCBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalIn this work, a flow-based spectrofluorimetric method for iodine determination was developed. The system consisted of a miniaturized chip-based flow manifold for solutions handling and with integrated spectrofluorimetric detection. A multi-syringe module was used as a liquid driver. Iodide was quantified from its catalytic effect on the redox reaction between Ce(IV) and As(III), based on the Sandell–Kolthoff reaction. The method was applied for the determination of iodine in salt, pharmaceuticals, supplement pills, and seaweed samples without off-line pre-treatment. An in-line oxidation process, aided by UV radiation, was implemented to analyse some samples (supplement pills and seaweed samples) to eliminate interferences and release iodine from organo-iodine compounds. This feature, combined with the fluorometric reaction, makes this method simpler, faster, and more sensitive than the classic approach of the Sandell–Kolthoff reaction. The method allowed iodine to be determined within a range of 0.20–4.0 µmol L<sup>−1</sup>, with or without the in-line UV digestion, with a limit of detection of 0.028 µmol L<sup>−1</sup> and 0.025 µmol L<sup>−1</sup>, respectively.https://www.mdpi.com/1420-3049/27/4/1325iodinespectrofluorimetrychip-based manifoldmulti-syringe flow systemin-line UV digestionphotooxidation |
| spellingShingle | Joana L. A. Miranda Raquel B. R. Mesquita Edwin Palacio José M. Estela Víctor Cerdà António O. S. S. Rangel Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples Molecules iodine spectrofluorimetry chip-based manifold multi-syringe flow system in-line UV digestion photooxidation |
| title | Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples |
| title_full | Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples |
| title_fullStr | Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples |
| title_full_unstemmed | Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples |
| title_short | Chip-Based Spectrofluorimetric Determination of Iodine in a Multi-Syringe Flow Platform with and without In-Line Digestion—Application to Salt, Pharmaceuticals, and Algae Samples |
| title_sort | chip based spectrofluorimetric determination of iodine in a multi syringe flow platform with and without in line digestion application to salt pharmaceuticals and algae samples |
| topic | iodine spectrofluorimetry chip-based manifold multi-syringe flow system in-line UV digestion photooxidation |
| url | https://www.mdpi.com/1420-3049/27/4/1325 |
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